African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12359

Full Length Research Paper

Phytochemical and in-vitro antioxidant properties of ethyl acetate leave extract of Dryopteris dilatata on Wistar rats

Akpotu Ajirioghene
  • Akpotu Ajirioghene
  • Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ani Celestine
  • Ani Celestine
  • Department of Human Physiology, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Enugu State, Nigeria.
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Agiopu Toby
  • Agiopu Toby
  • Department of Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Okeke Adaobi
  • Okeke Adaobi
  • Department of Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Agu Francis
  • Agu Francis
  • Child Health Department, University of Benin Teaching Hospital, Benin?City, Edo State, Nigeria.
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Ugwuchukwu Nnaemeka
  • Ugwuchukwu Nnaemeka
  • Department of Medicine, University of Benin Teaching Hospital, Benin?City, Edo State, Nigeria.
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Ugwudike Patrick5,
  • Ugwudike Patrick5,
  • Medical Biochemistry Department, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Enugu State, Nigeria.
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Eze Chukwuka
  • Eze Chukwuka
  • Medical Biochemistry Department, College of Medicine, Enugu State University of Science and Technology, Parklane Enugu, Enugu State, Nigeria.
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Uzoigwe Jide
  • Uzoigwe Jide
  • Department of Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Anyaeji Pamela
  • Anyaeji Pamela
  • Department of Physiology, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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Ghasi Samuel
  • Ghasi Samuel
  • Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria.
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  •  Received: 08 March 2021
  •  Accepted: 30 July 2021
  •  Published: 31 August 2021

Abstract

Medicinal plants contain natural bio-active constituents in various parts called photochemical that are of therapeutic importance. This research was undertaken to determine the phytochemical constituents and in-vitro antioxidant activities of ethyl acetate extract of Dryopteris dilatata leaves (DDL). Phytochemical analysis was determined on the plant samples following established protocols. The antioxidant property of Dryopteris dilatata leaves was evaluated using ferric reducing antioxidant power (FRAP), superoxide scavenging radical activity (SSRA) and hydroxyl radical activity (OH) assay. The results of the phytochemical analysis revealed the presence of the following phenols, terpenoid, alkaloids, flavonoids, tannins, cardiac glycosides and saponins, while steroids, carbohydrates, proteins, amino acids and phlobotannins were below detectable levels. DDL leaves produced significant (P<0.05) levels of ferric reducing antioxidant power (FRAP), superoxide scavenging radical activity (SSRA) and hydroxyl radical activity (OH) in a concentration dependent manner compared to the reference antioxidants, ascorbic acid and manitol; therefore, the extract could serve as free radical scavenger, acting as primary antioxidants. Based on the phytoconstituents and antioxidant activities, it could be concluded that D. dilatata could be of great value in the management of hyperglycemia, hyperlipidemia, and cancer, among other diseases that could be caused as a result of oxidative stress. This calls for further exploration of its bioactive compound.

 

Key words: Phytochemicals, Dryopteris dilatata, in-vitro antioxidant and, oxidative stress.