African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Molecular diversity study of black cumin (Nigella sativa L.) from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers

Birhanu Kapital*
  • Birhanu Kapital*
  • Biology Department, College of Natural and Computational Sciences, Debre Berhan University, P. O. Box 445, Debre Berhan, Ethiopia.
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Tileye Feyissa
  • Tileye Feyissa
  • Institute of Biotechnology, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia.
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Yohannes Petros
  • Yohannes Petros
  • Biology Department, College of Natural and Computational Sciences, Haramaya University, P. O. Box 138, Haramaya, Ethiopia.
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Said Mohammed
  • Said Mohammed
  • Biology Department, College of Natural and Computational Sciences, Debre Berhan University, P. O. Box 445, Debre Berhan, Ethiopia.
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Abdie Oumer*
  • Abdie Oumer*
  • Assosa University, Department of Biology, Assosa, Ethiopia.
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Petros Yohannes
  • Petros Yohannes
  • Haramaya University, Department of Biology, Haramaya, Ethiopia.
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Tesfaye Kassahun
  • Tesfaye Kassahun
  • Institute of Biotechnology, Addis Ababa University, Ethiopia.
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Teshome Abel
  • Teshome Abel
  • Addis Ababa University, Science Faculty, Addis Ababa, Ethiopia.
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Bekele Endashaw
  • Bekele Endashaw
  • Addis Ababa University, Science Faculty, Addis Ababa, Ethiopia.
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  •  Received: 14 March 2015
  •  Accepted: 27 April 2015
  •  Published: 06 May 2015

Abstract

Nigella sativa L. (commonly known as black cumin) belonging to family Rannunculaceae is an important medicinal plant with worldwide distribution. In Ethiopia, N. sativa occurs in all regions and agro-ecologies at different altitudinal ranges. This plant has a lot of importance in Ethiopia. However, there is no information available on molecular genetic diversity of this crop in respect to Ethiopia. Therefore, the aim of this study was to investigate the genetic diversity of black cumin populations collected from Amhara, Oromia, Tigray, Benshangul and South Nation’s Nationality People regions of Ethiopia using ISSR marker. A total of 84 black cumin accessions were obtained from Institute of Biodiversity Conservation (IBC). The ISSR marker was used for computing gene diversity, percent polymorphism, Shannon diversity index and AMOVA. Overall, accessions from Oromia showed the highest gene diversity (H = 0.35) and Shannon information index (I= 0.52), followed by Amhara with gene diversity and Shannon index values of (0.35) and (0.51), respectively. NJ and UPGMA results showed strong grouping among accessions collected from the Oromia and Amhara region. The five geographical regions of Ethiopia showed different levels of genetic variation. Thus, conservation priority should be given for those regions that have low genetic diversity.

 

Key words: Conservation, indigenous, molecular markers, primers.