African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12400

Full Length Research Paper

Exploration of indigenous agrowastes for cellulase production by Aspergillus niger

Umbrin Ilyas1*, Shakil Ahmed1, Abdual Majeed2 and Muhammad Nadeem3
  1Institue of Plant Pathology, University of the Punjab, Lahore (54000), Pakistan. 2Department of Botany, University of Gujrat, Gujrat (50700), Pakistan. 3Pakistan Council of Scientific & Industrial Research, Lahore (54000), Pakistan.
Email: [email protected]

  •  Accepted: 28 September 2011
  •  Published: 10 May 2012

Abstract

 

Regional agrowastes such as Vigna mungo, Saccharum spontaneum and Brassica campestris were collected and biohydrolysis of these substrates for cellulase production were carried out by Aspergillus niger. Proximate composition of each agrowastes was analyzed based on dry weight, to have an insight view of their chemical composition. Cellulose content of S. spontaneum and B. campestris was calculated as 43.1 ± 2.8 and 39.4 ± 3.1%, respectively, while cellulase activity of A. niger was found to be higher on S. spontaneum. Low lignin to cellulose ratio of S. spontaneum made it a preferred substrate for cellulase production. However, high lignin content of B. campestris made the cellulose inaccessible and resulted in poor yield of enzyme. Therefore, S. spontaneum has a great potential to serve as a cheaper, easily available and reasonable substrate for cellulase production.

 

Key words: Agrowastes, cellulase, indigenous, Vigna mungo, Saccharum spontaneumBrassica campestris.