Triterpenoid production and plant defense enzyme activity in suspension cultures ofBetula platyphylla Suk. cells treated with chitosan elicitor were investigated.Chitosan, at the optimal concentration of 100 mgL-1 enhanced triterpenoid production by 2.6-fold, with a maximum yield of 202.75 versus 78.72 mgL-1 of the untreated control cells. Furthermore, the activities of superoxide dismutase, phenylalanine ammonialyase, endochitinases and exochitinases increased by 1.6 ~ 3.0 fold. These results indicate that triterpenoid accumulation in B. platyphylla Suk. cell are the consequence of a plant defense response to chitosan elicitation.
Key words: Chitosan, Betula platyphylla Suk., defense enzymes, triterpenoids.
PAL, Phenylalanine ammonia lyase; NBT, nitro blue tetrazolium reaction; MAMP, microbe associated molecular pattern; SOD, superoxide dismutase; ROS, reactive oxygen species; PR, pathogenesis-related; EDC,endochitinase; EOC, exochitinase.
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