African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Article in Press

Assessment of variability among Striga hermonthica populations in Sudan using SSR markers

MOHAMMED ESHAG BABIKER*1, Kyalo Martina Kyalo2, Yao Nasir Nasir3

  •  Received: 07 November 2018
  •  Accepted: 12 April 2019
Striga weed is considered to be the greatest biological constraint to food production in sub-Saharan Africa (SSA). Striga hermonthica is the most widespread among the species in the semi-arid tropical African zones. Seven populations of S. hermonthica were collected from five regions on sorghum and millet in Sudan and investigated with 12 microsatellite markers. Host specificity or geography may play important roles in shaping the population structure of S. hermonthica. 108 fragments were detected across all primer combinations and numbers per each primer ranged from 2 to 18 with average 9.1 fragments. The genetic diversity among the seven populations was moderate as revealed by the Nei‟s genetic distance values which, ranged from 0.387 to 0.919 with an average of 0.724. Genetic differentiation between populations was relatively high, and all populations were significantly different from each other. FST values ranged from 0.00 to 0.643 indicating high differentiation. Geography appeared to play a significant role in shaping the genetic diversity of S. hermonthica.

Keywords: Striga hermonthica, SSR, host specificity, geography, Sudan.