African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12257

Article in Press

Characterization of crude invertase extracted from Aspergillus species CSA 35

Ohwofasa Aghogho¹*, Aliyu Abubakar Aliyu¹, Enoch Kwaku Larrey¹ and Ogbodu Ogaga Daniel²

  •  Received: 31 July 2019
  •  Accepted: 29 August 2019
The enzyme invertase is very useful, especially in food industries. Invertase hydrolyzes the disaccharide sucrose into two monosaccharides, glucose and fructose. Invertase was extracted from Aspergillus sp. CSA35, and studied using spectrophotometric methods for enzyme kinetics and determination of other factors affecting enzyme activities such as salt tolerance, pH, temperature and substrate specificity. The invertase secreted by Aspergillus sp. CSA35 has a substrate concentration (Km) value of 1.25mg/ml and maximum velocity (Vmax) of 15.15 U/mg protein. It is tolerant to salt concentrations of 3 M NaCl for 24 hours, shows a broad range of substrate specificity. The enzyme activity was optimal at pH 6.0 and at a temperature of 50ºC. The Vmax of the enzyme was high compared to other invertases from fungal sources, previously reported in literature. This invertase could be useful in commercial processes where high activity invertase with salt-tolerant and broad specific properties are required.

Keywords: Aspergillus sp CSA35, Invertase, Manihot esculenta