African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Article in Press

Direct and indirect somatic embryogenesis from leaf and leaf derived callus of Momordica dioica Roxb

T. N. Swamy1, Bylla Prasad2, V. Suresh1, V. Sadguna1, G. Kavitha1 and Md. Mustafa1

  •  Accepted: 22 December 2014
The protocol was developed for both direct and indirect somatic embryogenesis from leaf explants and leaf derived callus of Momordica dioica. Direct somatic embryos were achieved form leaf explants after 5 weeks of culture on MS medium supplemented with 2.0 mg/l TDZ+2.0 mg/l L-Glutamic acid and influenced by other factors. This is the first report of this kind in M. dioica for the formation of somatic embryos directly from the explants. An indirect somatic embryogenesis was also achieved on MS medium supplemented with 2.0 mg/l TDZ + 1.0 mg/l NAA from leaf derived callus of M. dioica in 1st subculture. The embryogenic calli were vary in terms of color and morphology. This calli differentiated in to somatic embryos and these somatic embryos increased in size and developed into torpedo, heart and bipolar shaped on the same medium after 2nd subculture. Such somatic embryos were developed into new plants, and were rooted and transferred to field.

Keywords: MS medium, callus, in vitro leaf, somatic embryogenesis, Torpedo shaped embryos, TDZ (Thiadizuron), L-Glutamic acid and NAA (Naphthalene acetic acid).