African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Article in Press

Diversity and genetic structure in natural populations of Croton linearifolius Croton linearifolius (Euphorbiaceae) based on molecular markers

Thalana Souza Santos Silva, Janaína Silva Freitas, Elisa Susilene Lisboa dos Santos and Carlos Bernard Moreno Cerqueira-Silva

  •  Received: 19 July 2018
  •  Accepted: 24 May 2019
Croton linearifolius Mull. Arg, an endemic species of Brazil, has insecticidal activity proven. To the detriment of its importance as a natural resource, the studies of this species are incipient, as well as the strategies applied to its management and conservation. Were estimated the diversity and genetic structure in 61 individuals of C. linearifolius collected in the National Forest Contendas do Sincor√° (NFCS). Estimates were based on analysis of the amplification profile of nine combinations of pairs of RGA (Resistance Gene Analogs) primers and eight ISSR (Inter Simple Sequence Repeat) primers. A total of 134 markers (81.3% polymorphic) were generated. Bayesian analysis indicated as most likely a structuring into two groups. Based on the molecular analysis of variance (AMOVA) it was possible to verify that 64% (p rand <0.01) of the variation occurs within the regions, and a significant amount (36%) (p rand <0.01) was attributed to variations between regions, indicating genetic structure between them. The AMOVA results were corroborated with the Principal Coordinate Analysis (PCoA), indicating an association between the distribution of variability and the geographical distribution of the collection regions. The probable mechanisms pollination and dispersion would justify, at least in part, the genetic structuring observed for C. linearifolius.

Keywords: Conservation, Genetic Variability, Genetic polymorphism, RGA marker, ISSR marker.