The high distribution of resistance of a wide range of microorganisms to the diverse range of antimicrobial agents is attracting a great deal of attention. Development of effective and safe drugs based on nanoprinciples are now extensively utilized to fight against pathogenic microorganism.Among the various route ,infection through cross contamination on various objects including wound dressing causes severe health risks in hospatalized patients. In the present study, biocompatible nano formulated anti bacterial antibiotic coated on wound dressing and the effect of coating on mechanical properties and anti bacterial activity has been carried out. Biogenic silver nanoparticles-levofloxacin nano drug conjugate stabilized with chitosan was coated on wound dressing by pad dry cure method. and the effect of coating on tensile strength, the bursting strength and air permeability.Coated wound dressing Surface topography of coated wound dressing was characterized by scanning electron microscopy (SEM) which revealed the complete distribution of nano conjugate on the fibre surface with the size range of 70-85nm.Further characterization by fourier transform infrared spectroscopy (FTIR) showed characteristic modification in the absorbed peaks. Distinct changes in physical properties was not recorded except air permeability.Nano formulation coating showed increased air permeability. Anti bacterial activity of the nano formulated drug coated wound dressing showed distinct pattern of inhibition. Keywords. Nano formulation,Wound dressing,Anti bacterial,Mechanical properties,Inhibition