In vitro rooting of micropropagated shoots were carried out with the aim of evaluating the root induction responses of two tomato varieties (Gelilema and Chali) to Indole -3- butyric acid (IBA). Seven levels of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg L-1) were used in a completely randomized design (CRD) in factorial combinations (seven level of IBA*two varieties) with three replications. The ANOVA showed that the interaction of IBA*Var was highly significantly different for rooting percentage, number of roots/shoot and root length. The highest rooting percentage (100.00±0.00), number of roots/shoot (14.20±0.35) and root length (10.7±0.29) were received from Chali on free Murashige and Skoog (MS) medium. While the lowest rooting percentage (11.11±0.00), number of roots/shoot (0.887±0.19) and root length (1.00±0.00 cm) was obtained from Gelilema on MS+1.5 mg/L IBA. For acclimatization the in vitro rooted shoots were transplanted into plastic pots containing a mixture of soil and sand at a ratio of 2:1. After three weeks a survival rate of 67.7% for Chali and 58.1% for Gelilema was obtained. Free MS medium was the best for in vitro rooting of the two tomato varieties. The optimized protocol will be useful for rapid in vitro rooting of the two varieties.
Keywords: In vitro rooting; Lycopersicon esculentum; Tomato; Gelilema, Chali, Indole-3-butyric acid