African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12404

Article in Press

Effects of 2-Chlorophenol and 2,4-Dichlorophenol on Mitotic Chromosomes and Nucleic Acid Content Using Allium cepa and Vicia faba Assays


  •  Received: 27 December 2021
  •  Accepted: 20 June 2022
Chlorophenols are compounds used as intermediaries in manufacturing agricultural pesticides. Despite the efficiency on their targets, they considered as environmental pollutant substances. This study assessed two chlorophenol substances, 2-chlorophenol and 2,4-dichlorophenol (2-CP, 2,4-DCP) for their cytogenetic, genotoxic, and nucleic acid content affects using Allium cepa and Vicia faba assay. In Allium cepa, 2-CP low dosages (0.625%) increased the Mitotic index (MI), while high dosages (1.25 & 2.5%) decreased it; the high dosage (2.5%) increased the chromosomal aberration frequency (CAF). Treatment with 2,4-DCP showed that (2.6%) was most affected treatment, it decreased (MI) and increased (CAF). In Vicia faba, both 2-CP and 2,4-DCP decreased (MI) and increased (CAF). Types of chromosome anomalies that scored after treatment with different concentrations were, chromosome stickiness, disturbance, lagging chromosomes, anaphase and telophase bridge, and micronuclei. The contents of DNA and RNA in Allium cepa were increased by 2-CP and 2,4-DCP, and decreased by the half dosage of 2-CP (1.25%); the RNA content varied directly proportional to DNA, while in Vicia faba, all tested concentrations of chlorophenols increased nucleic acids contents, and (1.3%) was significantly affected. The results showed that the two substances have cytogenetic, genotoxic impact, and also have influence on the nucleic acid contents in both plants.

Keywords: Cytogenetic. Genotoxic. Chlorophenols. Allium cepa. Vicia faba.