The ultimate aim of this study is to standardize the conditions for in vitro organogenesis of tomato using plant growth regulators, organic elicitors, polyamines and seaweed extracts. Initially excised leaf explant was cultured on to the medium containing different concentrations of auxins. The best responded auxin was tested in combination with efficient cytokinins (Kinetin [Kin], 6-Benzylaminopurine [BAP] and Thidiazuron [TDZ]) at different concentrations (0.5 to 3.0 mg/L). To achieve the best organogenic capability, different elicitors (casein hydrolysate, yeast extract, glutamine, adenine sulfate), polyamines (putrescine, spermidine and spermine) and seaweeds (Gracilaria edulis and Sargassum wightii) were tested at various concentrations. The presence of plant growth regulators in seaweed extracts were confirmed by Fourier transform infrared (FTIR) and high performance liquid chromatography (HPLC) analysis. HPLC analysis of seaweed extracts and standard plant growth regulators revealed the presence of 1-naphthaleneacetic acid (NAA) and 2iP in S. wightii. The standard chromatogram of NAA was in correlation with the chromatogram of G. edulis. Hence this regeneration system provides an additional platform for generating transgenic plant in an efficient manner.

Keywords: Tomato, organogenesis, plant growth regulators, seaweeds, polyamines.