The objective of this work was to propagate three species of Eucalyptus: Eucalyptus camaldulensis, Eucalyptus gomphocephala and Eucalyptus maideni on a large scale using microcutting technique. For the test of microcutting, from in vitro germination, the best rate of multiplication of 4.75 were obtained for E. camaldulensis on a medium containing 1 mg/L 6-benzylaminopurine (BAP) and 1 mg/L ï¡-aminoisobutyric acid (AIB) in the third subculture. The microcutting has 100% rate of proliferation. Callus regeneration was observed when grown on media containing growth regulators. Plant regeneration from callus was successful for three species. This callus put on culture medium, developed broken leaf growths in the rivet washer only in the medium M1, M2 and M3 for the three species and of the roots on certain cal of the medium M3 for E. camaldulensis. The rooting was easy and obtained on an MS medium (100%), without growth regulators. For the test of microcutting, cuttings were taken from young seedlings of Eucalyptus cultivated under glazed greenhouse; the best rate of multiplication was 2.75 and was recorded for E. gomphocephala and E. maideni with the 2nd subculture for a report/ratio AIB/BAP of 1/1 (M3). Moreover, the callogenesis observed on all the media was 100%, even on MS medium not supplemented with the growth hormones. For rooting, no result was obtained on the various mediums used.
Keywords: Eucalyptus, in vitro culture, microcutting, micropropagation.
