Fish has been estimated to be 17% worldwide animal protein consumption. Fish and its products, nevertheless, have been established as a transporter of food borne diseases. This study was undertaken to determine the incidence, prevalence and antimicrobial sensitivity pattern of Salmonella enterica and Pseudomonas aeruginosa recovered from fish. Fifty (50) samples of fish were collected from Central Market, Gamji Gate and Station Market. From the fish samples collected, 28 were catfish and 22 were tilapia. Bacteria were isolated from the body, gills and intestine of the fishes using selective media for Salmonella and Pseudomonas. All isolates were initially characterized by cultural and biochemical techniques as species of Pseudomonas aeruginosa and Salmonella enterica. DNA were extracted using phenol chloroform procedure and 16S rRNA gene was dilated by Polymerase Chain Reaction (PCR) using Nano drop spectrophotometer. The sequence result obtained using DNA sequencing machine was sequenced with BLAST search of NCBI database to confirm the existence of Salmonella enterica and Pseudomonas aeruginosa. The findings in this study have documented fish contamination with some human pathogens, Salmonella enterica and Pseudomonas aeruginosa.

Keywords: Antimicrobial, pathogens, occurrence, prevalence