African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12368

Article in Press

Molecular Characterization, Optimization of Polyhydroxybutyrate (PHB) production using agro-wastes as substrate by Bacillus cereus

Jasmine R, Hannah S

  •  Received: 11 February 2020
  •  Accepted: 08 May 2020
Non biodegradability of plastics has discouraged its use and hence alternative eco friendly polyhydroxybutyrates have gained popularity. The use of cost effective and easily available substrates in the production of polyhydroxy butyrate (PHB) enhances its application and further reduces its cost and hence PHB can be efficiently used instead of plastics. The present work was performed to study the optimum production of polyhydroxy butyrate (PHB) by Bacillus cereus using cheap vegetable and fruit peels (apple and beet-root peels) as carbon sources and the efficiency was compared with glucose, sucrose and lactose as carbon sources at different time intervals. The maximum PHB production was observed with glucose as carbon source. The increase was observed up to 48h and then a decline was noted. Among the two peels used as carbon source for PHB production, beetroot peel was found to give a better yield than apple peel. Thus agro-wastes could be successfully used as alternate sources of substrates for PHB production. Further investigations are undertaken.

Keywords: Polyhydroxy butyrate, sudan black, agrowastes, biodegradable