African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12368

Article in Press

The adults of Anthonomus grandis as disseminator of Fusarium chlamydosporum and use of oregano extracts for their control

Jesus DiCarlo Quiroz Velasquez; J. Luis Hernandez-Mendoza, Guadalupe Rodriguez Castillejos; Gerardo Sanchez Ramos; Cristian Lizarazo Ortega; Jesus Gerardo Garcia Olivares

  •  Received: 11 February 2020
  •  Accepted: 08 May 2020
The Cotton Weevil is an insect that causes considerable losses to the cotton industry. Its larvae grow inside the cotton bolls, damaging them. It enters diapause during the winter and becomes active again in the first months of the year. Pheromone traps can be used to estimate their populations and implement control measures. This work analyzes samples of weevil populations collected in traps placed on mexican Tamaulipas-Texas border. On the weevil population a fungus was detected growing in the intersegmental areas of the abdomen and legs of the insect, which is characteristic of entomopathogenic fungi. This fungus was only present on the specimens collected in March and absent on the populations collected in August and September. The statistical analysis showed that there were highly significant differences between treatments (mycosed populations per month). The adult’s weevil was placed in a humid chamber and the fungus obtained were isolated and identified by PCR using ITS1 and ITS4. Sequence analysis showed that the fungi correspond to Fusarium chlamydosporum. After, the ethanolic extracts of Mexican oregano were evaluated for their ability to control of this phytopathogen. The results showed that the growth of the fungus was smaller in the Viad sample, with 1.23 cm of growth, which differs significantly fron the values of samples Rey01 and Rey02. The control sample had a growth of 4.60 cm a difference of 3.37 cm with respect to Viad sample. This is the first report of A grandis dispersing Fusarium chlamidosporum and the antifungal activity of ethanolic extracts of L. graveolens on this fungus.

Keywords: Antagonism, Cotton weevil, reservoir, thymol, carvacrol, biological control.