The application of modern biotechnology for improvement of pepper productivity requires an efficient in vitro plant regeneration protocol. In this study an experiment was carried out to examine the effects of different combinations of plant growth regulators on the in vitro micro propagation of tow genotypes of Capsicum annuum L., ( Z215, Y195) thus optimizing the protocol for in vitro micro propagation culturing of nodal segments in MS media. Among the different concentrations of plant regulator (Zea، KIN، BA . IBA,NAA ) The best response was observed on medium containing 2 mg/l BA and 0.5 mg/l IBA, especially for number of buds per explant and percentage of rooting in Varity Z215 then Y195 The elongated shoots were rooted on medium containing IBA (0.5 mg/l). Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and growing to maturity bearing normal fruits with seeds.