African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation and binary fusion of taro (Colocasia esculeneta (L) Schott.) protoplast: Towards developing somatic hybridization protocol as an alternative to sexual hybridization

Mohamed Chungwa
  • Mohamed Chungwa
  • Crops, Horticulture and Soils Department, Egerton University, P. O. Box 536-20115 Egerton, Njoro, Kenya.
  • Google Scholar
Robert Gesimba
  • Robert Gesimba
  • Crops, Horticulture and Soils Department, Egerton University, P. O. Box 536-20115 Egerton, Njoro, Kenya.
  • Google Scholar
Abwao S. Indieka
  • Abwao S. Indieka
  • Biochemistry and Molecular Biology Department, Egerton University, P. O. Box 536-20115 Egerton, Njoro, Kenya.
  • Google Scholar


  •  Received: 07 November 2023
  •  Accepted: 12 December 2023
  •  Published: 30 April 2024

References

Ahmed MAA, Miao M, Pratsinakis ED, Zhang H, Wang W, Yuan Y, Lyu M, Iftikhar J, Yousef AF, Madesis P, Wu B (2021). Protoplast isolation, fusion, culture and transformation in the woody plant Jasminum spp. Agriculture 11(8):699-719.
 Crossref

 

Barnes AC, Elowsky CG, Roston RL (2019). An Arabidopsis protoplast isolation method reduces cytosolic acidification and activation of the chloroplast stress sensor sensitive to freezing 2. Plant Signaling and Behavior 14(9):1-9.
Crossref

 
 

Chaïr H, Traore RE, Duval MF, Rivallan R, Mukherjee A, Aboagye LM, Van Rensburg WJ, Andrianavalona, V, Pinheiro de Carvalho MAA, Saborio F, Prana MS, Komolong B, Lawac F, Lebot V (2016). Genetic diversification and dispersal of taro (Colocasia esculenta (L.) schott). PLoS One 11(6):e0157712.
Crossref

 
 

Davey MR, Anthony P, Power JB, Lowe KC (2005). Plant protoplasts: status and biotechnological perspectives. Biotechnology Advances 23:131-171.
Crossref

 
 

Deo PC, Harding RM, Taylor M, Tyagi AP, Becker DK (2009). Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta). Plant Cell Tissue and Organ Culture 99:61-71.
Crossref

 
 

Deo PC, Tyagi AP, Taylor M, Harding R, Becker D (2010). Factors affecting somatic embryogenesis and transformation in modern plant breeding. The South Pacific Journal of Natural and Applied Sciences 28(1):27-40.
Crossref

 
 

Dutt M, Mahmoud LM, Chamusco K, Stanton D, Chase CD, Nielsen E, Quirico M, Yu Q, Gmitter FG, Grosser JW (2021). Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica). PLoS One 16(8):e0255842.
Crossref

 
 

FAOSTAT (2021). Food and Agriculture Organization [WWW Document]. FAOSTAT. Available at:  View

 
 

Grimaldi IM, van Andel TR (2018). Food and medicine by what name? Ethnobotanical and linguistic diversity of taro in Africa. Economic Botany 72:217-228.
Crossref

 
 

Henriquez CL, Arias T, Pires JC, Croat TB, Schaal BA (2014). Phylogenomics of the plant family Araceae. Molecular Phylogenetics and Evolution 75:91-102.
Crossref

 
 

Millam S, Payne LA, Mackay GR (1995). The integration of protoplast fusion-derived material into a potato breeding programme: a review of progress and problems. Euphytica 85:451-455.
Crossref

 
 

Oladimeji JJ, Kumar PL, Abe A, Vetukuri RR, Bhattacharjee R (2022). Taro in West Africa: status, challenges, and opportunities. Agronomy 12(9):2094.
Crossref

 
 

Reed KM, Bargmann BOR (2021). Protoplast regeneration and its use in new plant breeding technologies. Frontiers in Genome Editing 3:734951.
Crossref

 
 

Verma VM, Cho JJ (2010). Plantlet development through somatic embryogenesis and organogenesis in plant cell cultures of Colocasia esculenta (L.) schott. Asia Pacific Journal of Molecular Biology and Biotechnology 18:165-168.

 
 

Ranaware AS, Kunchge NS, Lele SS, Ochatt SJ (2023). Protoplast Technology and Somatic Hybridisation in the Family Apiaceae. Plants 12(5):1060.
Crossref