African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Full Length Research Paper

Molecular characterization of cultivated cowpea (Vigna unguiculata L. Walp) using simple sequence repeats markers

Ogunkanmi, L. A.*
  • Ogunkanmi, L. A.*
  • Cell biology and Genetics Department, University of Lagos, Nigeria.
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Ogundipe, O. T.
  • Ogundipe, O. T.
  • Botany Department, University of Lagos, Nigeria.
  • Google Scholar
Fatokun, C. A.
  • Fatokun, C. A.
  • International Institute of Tropical Agriculture IITA Ibadan, Nigeria.
  • Google Scholar

  •  Received: 17 August 2013
  •  Accepted: 08 August 2014
  •  Published: 20 August 2014


Aaron TA, Gowda BS, Galyuon IKA, Aboagye LL, Takrama JF, Timko MP (2010) Assessment of the genetic diversity in cowpea (Vigna unguiculata L. Walp.) germplasm from Ghana using simple sequence repeat markers. Plant Genet. Resour. 8 (02):142-150.
Adewale BD, Adeigbe OO, Aremu CO (2011). Genetic distance and Diversity among some Cowpea (Vigna unguiculata L. Walp) genotypes Int. J. Res. Plant Sci. 1(2):9-14.
Amin B, Noroozi S, Javaran MJ (2010) Study on genetic diversity of some Iranian Pistachio (Pistacia vera L.) cultivars using random amplified polymorphic DNA (RAPD), inter sequence repeat (ISSR) and simple sequence repeat (SSR) markers: A comparative study. Afr. J. Biotechnol. 9(45):7632-7640.
Andargie M., Pasquet RS, Gowda BS, Muluvi GM, Timko MP (2011). Construction of a SSR-based genetic map and identification of QTL for domestication traits using recombinant inbred lines from a cross between wild and cultivated cowpea (Vigna unguiculata (L.) Walp) Mol. Breed. 28:413-420.
Badiane FA, Gowda BS, Cissé N, Diouf D, Sadio O, Timko MP (2012). Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers. Genet. Mol. Res.11(1):292-304.
Becker J, Heun M (1995). Barley microsatellites: allele variation and mapping. Plant Mol. Biol. 2:835-845.
Broun P, Tanksley SD (1996). Characterization and genetic mapping of simple repeat sequences in the tomato genome Mol. Gen. Genet. 250:39-49.
Charcosset A, Moreau L (2004). Use of Molecular Markers for the development of new cultivars and the evaluation of genetic diversity. Euphytica 137:81-94
Dellaporta SL, Wood J, Hicks JB (1983). A plant minipreparation: version II. Plant Mol. Biol. Rep. I: 19-21.
Fatokun MS, Casa AM, Wang T, Mitchell SE, Dean RE, Kochert GD, Kresovich S (1999). Discovery and characterization of Polymorphic Simple Sequence Repeats (SSRs) in Peanut. Crop Sci. 39:1243-1247.
Fatokun CA, Danesh D, Menancio-Hautea DI, Young ND (1993a). A linkage map for cowpea V. unguiculata (L) Walp) based on DNA markers (2n=22). Pages 6256-6258. In genetic maps 1992, A compilation of linkage and restriction maps of genetically studied organisms, edited by J.S. O'Brien, Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, USA. P. 54
Fatokun CA, Danesh D, Young ND, Stewart EL (1993b). Molecular taxonomic relationships in the genus Vigna based on RFLP analysis. Theoretical and Applied Genetic. 86:97-104.
Feleke Y, Pasquet RS, Gept P (2006). Development of PCR based chloroplast DNA markers to assess gene flow between wild and domesticated cowpea (Vigna unguiculata) Plant Syst. Evol. 262(1/2):75-87
Ibrahim AB, Padulosi S, Chabane K, Hadj-Hassan A, Dulloo E, Augusto MP, Porced du E (2007) Genetic diversity of Syrian pistachio (Pistacia vera L.) varieties evaluated by AFLP markers. Genet. Resour. Crop Evol. 54(8):1807-1816
Ibrahima Z, Doumbia RA, Asibuo JY (2013) Comparative Study of Cowpea Germplasms Diversity from Ghana and Mali Using Morphological Characteristics. J. Plant Breed. Genet. 1:3.
Kuruma RW, Kiplagat O, Ateka E, KARI-Katumani OG (2012). Genetic Diversity of Kenyan Cowpea Accessions Based on Morphological and Microsatellite Markers 12 Kari Scientific conference proceedings 2012_2 Final 061.
Kuruma RW, Kiplagat O, Ateka E, Owuoche G (2008). Genetic diversity of Kenyan cowpea accessions based on morphological and microsatellite markers. East Afr. Agric. For. J. 76:3-4.
Li CD, Fatokun CA, Ubi B, Singh BB, Scoles GJ (2001). Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers Crop Sci. 41:189-197.
Manggoel W, Uguru MI (2011). Comparative study on the phenology and yield components of two photoperiodic groups of cowpea (Vigna unguiculata (L.) Walp.) in two cropping seasons: Afr. J. Agric. Res. 6(23):5232-5241.
Moalafi AI, Asiwe JAN, Funnah SM (2010). Germplasm evaluation and enhancement for the development of cowpea (Vigna unguiculata (L.) Walp.) dual-purpose F2 genotypes. Afr. J. Agric. Res. 5(7):573-579.
Ng NQ (1995). Cowpea (Vigna unguiculata) In: J. Smartt and S. Simnionds Eds. Evolution of crop plant 2nd ed. Longman, London. pp. 326-332.
Ogunkanmi LA, Ogundipe OT, Ng NQ, Fatokun CA (2008). Genetic diversity in wild relatives of cowpea (Vigna unguiculata) as revealed by simple sequence repeats (SSR) markers. J. Food Agric. Environ. 6 (3&4):263-268.
Ogunkanmi LA, Ogundipe OT, Ng NQ, Scoles GJ, Fatokun CA (2007). Genetic diversity in yard-long-bean (Vigna unguiculata subspecies unguiculata cv-gr sesquipedalis) as revealed by simple sequence repeat (SSR) markers. J. Plant Breed. Genet. 62 (1):43-52
Ogunkanmi LA, Taiwo IA, Mogaji OL, Awobodede A, Eziashi EI, Ogundipe OT (2006). Assessment of genetic diversity among cultivated cowpea (Vigna unguiculata L Walp) cultivars from a range of localities across West Africa using agronomic traits. J. Sci. Res. Dev. 10:111-118.
Ott J (1999). Analysis of human Genetic Linkage Third Edition. The John Hopkins University Press. pp. 28.
Padulosi S, Bhag M, Bala Ravi S, Gowda J, Gowda KTK, Shanthakumar G, Yenagi N, Dutta M (2009). Food Security and Climate Change: Role of Plant Genetic Resources of Minor Millets. Indian J. Plant Genet. Resour. 22(1):1-16.
Padulosi S, Hoeschle-Zeledon I, Bordoni P (2007). Minor crops and underutilized species: lessons and prospects. In Crop wild relative conservation and use edited by Maxted N. Ford-Lloyd, BV Kell, SP, Iriondo JM, Dulloo ME and Turok. Eds.CAB International, Wallingford, UK. pp. 605-624.
Pasquet RS (2000) Allozyme diversity of cultivated cowpea Vigna unguiculata L Walp. Theor. Appl. Genet. 101 (1/2):211-219.
Prasanthi L, Geetha G, Ramya B, Jyothi N, RajaReddy K (2012). Evaluation of genetic diversity in cowpea Vigna unguiculata L Walp.genotypes using RAPD. Curr. Biotica 6(1):22-31
Rohlf FJ (1993). NTSYS-pc. Numerical taxonomy and multivariate analysis version 2.02j. Applied Biostatistics, New York. pp. 34-45.
Senior ML, Heun M (1993). Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome 36:884-889.
Wayne P, Gordon CM, Jim P (1996). Polymorphism revealed by simple sequence repeat. Trends Plant Sci. Rev. 1:215-222.