African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Efficient micropropagation of Citrus sinensis (L.) Osbeck from cotyledonary explants suitable for the development of commercial variety

Aseesh Pandey
  • Aseesh Pandey
  • G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, India.
  • Google Scholar
Sushma Tamta
  • Sushma Tamta
  • Plant Tissue Culture Laboratory, Department of Botany, D.S.B. Campus, Kumaun University, Nainital, 263002, Uttarakhand, India.
  • Google Scholar

  •  Received: 18 September 2015
  •  Accepted: 30 October 2015
  •  Published: 24 August 2016


Ali S, Mirza B (2006). Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explant type and hormone concentration Acta Bot. Croat. 65 (2):137–146.


Barlass M, Skene KGM (1999). Citrus. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer, Berlin, pp. 207-219.


Bekele S (2007). Identification of citrus (Citrus sinensis) postharvest pathogens from ethiopia and their control. Ph.D. Thesis. University of Pretoria.


Bove JM (2006). Huanglongbing: a destructive, newly-emerging, century-old disease of Citrus. J. Plant Pathol. 88:7–37.


Carimi F, De Pasquale F (2003). Micropropagation of citrus. In SM Jain, K Ishii, eds, Micropropagation of Woody Trees and Fruits, Kluwer, Netherlands, pp. 589-619.


Chaudhary MI (1994). Fruit Crops. In: Malik M.N. (ed.), Horticulture, 422. National Book Foundation, Islamabad.


Costa MGC, Adves VS, Lani ERG, Carvalho CR, Otoni WC (2004). Morphogenic gradients of adventitious bud and shoot regeneration in epicotyl explants of Citrus. Sci. Hortic. 100:63-74.


Costa MGC, Otoni WC, Moor GA (2002). An elevation of factors affecting the efficieny of Agrobacterium-mediated transformation of Citrus paradisi (Macf.) and production of transgenic plants containing carotenoid biosynthetic genes, Plant Cell Rep. 21:365-373.


Crowell PL (1999). Prevention and therapy of cancer by dietary monoterpenes. J. Nutr. 129(3):775S-778S.


Das A, Paul AK, Chaudhuri S (2000). Micropropagation of sweet orange (Citrus sinensis Osbeck) for the development of nucellar seedlings. Indian J. Exp. Biol. 38:269-272.


Deng XX, Yu GH, Guo WW (2000). Somatic hybridization between diploids and allotetraploid somatic hybrids in Citrus. 9th ISC Congress Sun City Resort, South Africa. 54:115-121


Duran-Vila N, Ortega V, Navarro L (1989). Morphogenesis and tissue culture of three citrus species. Plant Cell Tissue Organ Cult. 16:123-133.


Ejaz S, Ejaz A, Matsuda K, Chae WL (2006). Limonoids as cancer chemopreventive agents. J. Sci. Food Agric. 86:339-345.


Elangovan V, Sekar N, Govindasamy S (1994). Chemopreventive potential of dietary bioflavonoids against 20-methylcholanthreneinduced tumorigenesis. Cancer Lett. 87:107-113.


Fawcett HS (1933). New symptoms of psoriasis, indicating a virus disease of Citrus. Phytopathology 23:930.


Food and Agriculture Organization of the United Nations (2006). (FAO Statistics),


Germana MA, Micheli M, Chiancone B, Macaluso L, Standardi A (2011). Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. x Poncirius trifoliate (L.) Raf]. Plant Cell Tissue Organ Cult. 106:299-307


Gill MIS, Singh Z, Dhillon BS, Gosal SS, Singh Z (1994). Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of kinnow mandarin (Citrus nobilis Lour × Citrus deliciosa Tenora). J. Hortic. Sci. 69:231-236.


Grosser JW, Chandler JL (2000). Somatic hybridization of high yield, cold-hardy and disease resistant parents for Citrus rootstock improvement. J. Hortic. Sci. Biotechnol. 75:641-644.


Honda H, Liu CZ, Kobayashi T (2001). Large-scale plant micropropagation. Adv. Biochem. Eng. Biotechnol 72:158-182.


Johnston JC (1968). Growing seedlings from seed. In: Opitz, K.W., Plate, R.G., Frolich, E.F. (eds.), Propagation of Citrus, Division of Agricultural Sciences University of California pp. 1-6.


Kaneyoshi J, Kobayashi S, Nakamura Y, Shigemoto N, Doi Y (1994). A simple and efficient gene transfer system to trifoliate orange (Ponicirus trifoliata Raf.). Plant Cell Rep. 13:541-545.


Khawale RN, Singh SK (2005). In vitro adventitive embryony in Citrus : A technique for Citrus germplasm exchange. Curr. Sci. 88(8):1309-1311.


Kiong ALP, Wan LS, Hussein S, Ibrahim R (2008). Induction of somatic embryos from explants different of Citrus sinensis. J. Sci. 3:18-32.


Koltunow AM (2002). Regeneration of West Indian Limes (Citrus aurantifolia) Containing genes for decreased seed set. Acta Hortic. 535:151-157.


Kotsias D, Roussos PA (2001). An investigation on the effect of different plant growth regulating compounds in in vitro shoot tip and node culture of lemon seedling. Sci. Hortic. 89:115-128.


Kotze JM (1981). Epidemiology and control of Citrus black spot in South Africa. Plant Dis. 65:945-950.


Kunta M, Palm M, Rascoe J, Desa PB, Timmer LW, Da Graça JV, Mangan RL, Malik NSA, Salas B, Satpute A, Sétamou M, Skaria M (2013). 'Sweet Orange Scab with a new scab disease "syndrome" of Citrus in the U.S.A. associated with Elsinoë australis. Trop. Plant Pathol. 38:203-212.


Mukhtar R, Khan MM, Fatima B, Abbas M, Shahid A (2005). In vitro regeneration and somatic embryogenesis in (Citrus aurantifolia and Citrus sinensis). Int. J. Agric. Biol. 7(2):414-416.


Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Cell Physiol. 15:473-497.


Pandey A, Tamta S (2013). Effect of pre-sowing treatments on seed germination in Quercus serrata Thunb. and Quercus semecarpifolia Sm. Int. J. Biodivers. Conserv. 5:791-795.


Pandey A, Tamta S (2014). In vitro propagation of the important tasar oak (Quercus serrata Thunb.) by casein hydrolysate promoted high frequency shoot proliferation. J. Sustain. For. 33:590-603.


Pandey A, Brijwal L, Tamta S (2013) In vitro propagation and phytochemical assessment of Berberis chitria: An important medicinal shrub of Kumaun Himalaya, India. J. Med. Plant Res. 7:930-937.


Pandey A, Negi D, Giri D, Tamta S (2011). In vitro seed germination study in Citrus sinensis. Oaks 7: 52-55.


Parmessur YS, Alijanabi S, Saumatally S, Dookun-samutually A (2002). Sugarcane yellow virus and sugarcane yellow phytoplasma: elimination by tissue culture. Plant Pathol. 51:561-569.


Paudyal KP, Haq N (2000). In vitro propagation of pummelo (Citrus grandis L. Osbeck), In Vitro Cell. Dev. Biol. Plant 36: 511-516.


Pena L, Cervera M, Juarez J, Navarro A, Pina JA, Duran-vila N, Navarro L (1995). Agrobacterium-mediated transformation of sweet orange and regeneration M Barlass M; KGM Skene. Citrus. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry.


Rossetti V (1977). Citrus canker in Latin America: a review. Proc. Int. Soc. Citricult. 3:918-924.


Sastra-hidayat AR (1992). Preliminary epidemiological study of powdery mildew (Oidium tingitaninum) on Citrus sinensis. Indonesian Center for Agricultural Library and Technology Dissemination, Pusat Perpustakaan dan Penyebaran Teknologi Pertanian (Indonesia) ICALTD Asian Citrus rehabilitation conference. Malang (Indonesia).


Te-Chato S, Nudoung S (1998). Tissue culture of Citrus reticulata Blanco CV. Shogun. and gene transformation by Agrobacteria. Proc. Abstracts IMT-GT UNITE Conf, Songkxhla.


Tripoli ELA, Guardia M, Giammanco S, Di Majo D, Giammanco M (2007). Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food Chem. 104:466-479.


Usman M, Muhammad S, Fatima B (2005). In vitro multiple shoot induction from nodal explants of Citrus cultivars. J. Cent. Eur. Agric. 6:435-442.


Walton BS, Bartholomew ET, Ramsey RC (1945). Analysis of the organic acids of orange juice. Plant Physiol. 20(1):3-18.