African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12428

Full Length Research Paper

Agrobacterium-mediated transformation of Jatropha curcas leaf explants with a fungal chitinase gene

Mariana Crotti Franco
  • Mariana Crotti Franco
  • Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13075-630, Brazil.
  • Google Scholar
Kleber Alves Gomes
  • Kleber Alves Gomes
  • Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13075-630, Brazil.
  • Google Scholar
Marcel Mamede de Carvalho Filho
  • Marcel Mamede de Carvalho Filho
  • Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13075-630, Brazil.
  • Google Scholar
Ricardo Harakava
  • Ricardo Harakava
  • Instituto Biológico, Secretaria de Agricultura e Abastecimento, Laboratório de Bioquímica Fitopatológica, Av. Conselheiro Rodrigues Alves, 1252, Vila Mariana, 04014002 - São Paulo, SP – Brazil.
  • Google Scholar
Nicolas Carels
  • Nicolas Carels
  • Fundação Oswaldo Cruz, Laboratório de Modelagem de Sistemas Biológicos, CDTS, Av. Brasil, 4365, Manguinhos, 21040-900 - Rio de Janeiro, RJ – Brazil.
  • Google Scholar
Walter José Siqueira
  • Walter José Siqueira
  • Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13075-630, Brazil.
  • Google Scholar
Rodrigo Rocha Latado
  • Rodrigo Rocha Latado
  • Centro APTA Citros Sylvio Moreira, Instituto Agronômico, Caixa Postal 04, Cordeirópolis, SP, 13490-970, Brazil.
  • Google Scholar
Daniela de Argollo Marques
  • Daniela de Argollo Marques
  • Centro de P&D de Recursos Genéticos Vegetais, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13075-630, Brazil.
  • Google Scholar


  •  Received: 09 May 2016
  •  Accepted: 07 September 2016
  •  Published: 14 September 2016

References

 

Argollo Marques DA, Siqueira WJ, Colombo CA, Ferrari RA (2013). Breeding and Biotechnology of Jatropha curcas. In: Bahadur B, Sujatha M, Carels N (eds). Jatropha, Challenges for a New Energy Crop (V2): Genet. Improv. Biotechnol. Springer Sci. pp. 457-478.
Crossref

 

Barik DP, Mohapatra U, Chand PK (2005). Transgenic grasspea (Lathyrus sativus L.): factors influencing Agrobacterium-mediated transformation and regeneration. Plant Cell Rep. 24:523-531.
Crossref

 
 

Basa SD, Sujatha M (2009). Genetic analysis of Jatropha species and interspecific hybrids of Jatropha curcas using nuclear and organelle specific markers. Euphytica 168:197-214.
Crossref

 
 

Beltrán J, Jaimes H, Echeverry M, Ladino Y, López D, Duque MC, Chavarriaga P, Tohme J (2009). Quantitative analysis of transgenes in cassava plants using real-time PCR technology. In Vitro Cell Dev Biol. Plant 45:48-56.
Crossref

 
 

Bhatia P, Ashwath N, Midmore DJ (2005). Effect of genotype, explant orintation, and wounding on shoot regeneration in tomato. In vitro Cellular Dev. Biol. Plant. 41:441-457.
Crossref

 
 

Bookout AL, Mangelsdorf DJ (2003). Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways. Nucl. Recept. Signal. 1 e 012.

 
 

Carels N (2013). Towards the domestications of Jatropha: The integration of sciences. In: Bahadur B, Sujatha M, Carels N (eds). Jatropha, Challenges for a New Energy Crop (V2): Genet. Improv. Biotechnol. Springer Sci. pp. 263-299.
Crossref

 
 

Casu RE, Selivanova A, Perroux JM (2012). High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR. Plant Cell Rep. 31:67-177.
Crossref

 
 

Debnath M, Bisen PS (2008). Jatropha curcas L., a multipurpose stress resistant plant with a potential for ethnomedicine and renewable energy. Curr. Pharm. Biotechnol. 9(4):288-306.
Crossref

 
 

Danilova SA, Dolgikh YI (2004). The stimulatory effect of the antibiotic cefotaxime on plant regeneration in maize tissue culture. Russ. J. Plant Physiol. 51:621-625.
Crossref

 
 

Deng JP, Yan SH, Hou P, Xu Y, Wang S, Tang L, Yan F, Chen F (2005). Effects of different antibiotics at various concentrations on culture of Jatropha curcas. Chin. J. Appl. Environ. Biol. 11:156-159.

 
 

Deore AC, Johnson TS (2008). High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotechnol. Rep. 2:7-11.
Crossref

 
 

Divakara BN, Upadhyaya HD, Wani SP, Laxmipathi Gowda CLL (2010). Biology and genetic improvement of Jatropha curcas L.: a review. Appl. Energ. 87:732-742.
Crossref

 
 

Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus. 12:13-15.

 
 

Flavell RB (1994). Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc. Natl. Acad. Sci. USA 91:3490–3496.
Crossref

 
 

Franco MC, Latado RR, Siqueira WJ, Marques DA (2014). Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry. Afr. J. Biotechnol. 13:3872-3880.
Crossref

 
 

Fu Q, Li C, Tang M, Tang M, Tao YB, Pan BZ, Zang L, Niu L, He, H, Wang X, Xu ZF (2015). An efficient protocol for Agrobacterium-mediated transformation for the biofuel plant Jatropha curcas by optimizing kanamycin concentration and duration of delayed selection. Plant Biotechnol. Rep. 9(6):405-416.
Crossref

 
 

Giersdorf J (2013). Politics and economics of ethanol and biodiesel production and consumption in Brazil. Accessed August 2016: View.

 
 

Guidolin AF (2003). Regeneração de plantas de Phaseolus vulgaris L. a partir de calos e transformação genética via Agrobacterium. 100f. Dissertação (Doutorado em Ciências) - Universidade de São Paulo, Piracicaba. Portuguese.

 
 

Hadi F, Salmanian AH, Ghazizadeh E, Amani J, Noghabi KA, Mousavi A (2012). Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants. Electron. J. Biotechnol. 15(4):2-2.

 
 

Herr J, Carlson J (2013). Traditional breeding, genomics-assisted breeding, and biotechnological modification of forest trees and short rotation woody crops. In: Jacobson M, Ciolkosz D, editors. Wood-based Energy in the Northern Forests. New York: Springer; pp. 79-99.
Crossref

 
 

Hobbs SLA, Kpodar P, DeLong CMO (1990). The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol. Biol. 15(6):851-864.
Crossref

 
 

Jha TB, Mukherjee P, Datta MM (2007). Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotechnol. Rep. 1:135-140.
Crossref

 
 

Jha B, Sharma A, Mishra A (2011). Expression of SbGSTU (Tau class glutathione S-transferase) gene isolated from Salicornia brachiata in tobacco for salt tolerance. Mol. Biol. Rep. 38:4823-4832.
Crossref

 
 

Jha B, Mishra A, Jha A, Joshi M (2013). Developing transgenic Jatropha using the SbNHX1 gene from an extreme halophyte for cultivation in saline wasteland. PLoS ONE. 8:8.
Crossref

 
 

Joshi M, Mishra A, Jha B (2011). Efficient genetic transformation of Jatropha curcas L. by microprojectile bombardment using embryo axes. Ind. Crop. Prod. 33:67-77.
Crossref

 
 

Kajikawa M, Morikawa K, Inoue M, Widyastuti U, Suharsono S, Yokota A, Akashi K (2012). Establishment of bispyribac selection protocols for Agrobacterium tumefaciens-and Agrobacterium rhizogenes-mediated transformation of the oil seed plant Jatropha curcas L. Plant Biotechnol. 29:145-153.
Crossref

 
 

Kaushik N, Kumar K, Kumar S (2007). Genetic variability and divergence studies in seed traits and oil content of Jatropha (Jatropha curcas L.) accessions. Biomass Bioenerg. 31:497-502.
Crossref

 
 

Kumar N, Reddy MP (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Ann. Appl. Biol. 156:367-375.
Crossref

 
 

Kumar N, Reddy MP, Sujatha M (2013). Genetic transformation of Jatropha curcas: Current status and future prospects. In: Bahadur B, Sujatha M, Carels N (eds). Jatropha, Challenges for a New Energy Crop (V2): ): Genet. Improv. Biotechnol. Springer Sci. Pp. 457-478.
Crossref

 
 

Kumar N, Vijay Anand KG, Sudheer Pamidimarri DVN, Sarkar T, Reddy MP, Radhakrishnan T, Kaul T, Reddy MK, Sopori SK (2010). Stable genetic transformation of Jatropha curcas via Agrobacterium tumefaciens-mediated gene transfer using leaf explants. Ind. Crops Prod. 32: 41-47.
Crossref

 
 

Kumar N, Vijayanand KG, Reddy MP (2011). Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants. J. Plant Biochem. Biotechnol. 20: 125-133.
Crossref

 
 

Lawrence PK, Koundal KR (2000). Simple protocol for Agrobacterium tumefaciens - mediated transformation of pigeonpea [Cajanus cajan (L.) Millsp.]. J. Plant Biol. 27(3):299-302.

 
 

Li MR, Li HQ, Jiang HW, Pan XP, Wu JG (2008). Establishment of an Agrobacterium-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss. Organ Cult. 92:173-181.
Crossref

 
 

Machado AA, Zonta EP (1995). Manual do SANEST: Sistema para análise estatística para microcomputadores. Pelotas, UFPel. 102.

 
 

Mason G, Provero P, Vaira AM, Acotto GP (2002). Estimating the number of integrations in transformed plants by quantitative real-time PCR. BMC Biotechnol. 2:20-30.
Crossref

 
 

Mathias RJ, Mukasa C (1987). The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgaris L.). Plant Cell Rep. 6:454-557.

 
 

da Silva Mendes AF, Cidade LC, de Oliveira MLP, Otoni WC, Soares-Filho WS, Costa MGC (2009). Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. Plant Cell Tiss. Organ Cult. 97:331-336.
Crossref

 
 

Miller JH (1972). Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York 295 p.

 
 

Misra P, Toppo DD, Mishra MK, Saema S, Singh G (2012). Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments. J. Plant Biochem. Biotechnol. 21:128-133.
Crossref

 
 

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant. 15:473-497.
Crossref

 
 

Nagl W, Ignacimuthu S, Becker J (1997). Genetic engineering and regeneration of Phaseolus and Vigna. State of the art and new attempts. J. Plant Physiol. 150:625-644.
Crossref

 
 

Nanasato Y, Kido M, Kato A, Ueda T, Suharsono, Widyastuti U, Tsujimoto H, Akashi K (2015). Efficient genetic transformation of Jatropha curcas L. by means of vacuum infiltration combined with filter-paper wicks. In vitro Cell. Dev. Biol. Plant 51:399-406.
Crossref

 
 

Nakano M, Mii M (1993). Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars. J. Plant Pathol. 141:721-725.

 
 

Oliveira MLP, Costa MGC, Silva CV, Otoni WC (2010). Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars. Pesq. Agropec. Bras. 45(7):654-660.
Crossref

 
 

Openshaw K (2000). A review of Jatropha curcas: an oil plant unfulfilled promise. Biomass Bioeng. 19:1-15.
Crossref

 
 

Pan J, Fu Q, Xu ZF (2010). Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection. Afr. J. Biotechnol. 9:6477-6481.

 
 

Pinheiro TT, Figueira A, Latado RR (2014). Early-flowering sweet orange mutant "x11" as a model for functional genomic studies of Citrus. BMC Res. Notes 7: 511.
Crossref

 
 

Purkayastha J, Sugla T, Paul A, Solleti SK, Mazumdar P, Basu A, Mohommad A, Ahmed Z, Sahoo L (2010). Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas. Biol. Plant. 54:13-20.
Crossref

 
 

Rao GR, Korwar GR, Shanker AK, Ramakrishna YS (2008). Genetic associations, variability and diversity in seed characters, growth, reproductive phenology and yield in Jatropha curcas (L.) accessions. Trees 22(5):697-709.
Crossref

 
 

Ramesh SA, Kaiser BN, Franks T, Collins G, Sedgley M (2006) Improved methods in Agrobacterium-mediated transformation of almond using positive (mannose/pmi) or negative (kanamycin resistance) selection-based protocols. Plant Cell Rep. 25:821-828.
Crossref

 
 

Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JC, Lapeyre F, Lashermes P, Etienne H (2011). Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol. 11:92.
Crossref

 
 

Rozen S, Skaletsky H (2000). Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics methods and protocols: methods in molecular biology. S. Misener and S.A. Krawetz, editors. Humana Press. Totowa, New Jersey, USA, pp. 365-386.

 
 

Sabandar CW, Ahmat N, Jaafar MJ, Sahidin L (2013). Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): A review. Phytochem. 85:7-29.
Crossref

 
 

Sangwan RS, Bourgeoiis Y, Brown S, Vasseur G, Sangwan-Norreel B. (1992). Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana. Plant. 188:439-456.
Crossref

 
 

Sharma S, Kumar N, Reddy MP (2011). Regeneration in Jatropha curcas: factors affecting the efficiency of in vitro regeneration. Ind. Crop Prod. 34:943-951.
Crossref

 
 

Stevens ME, Pijut, PM (2014). Agrobacterium-mediated genetic transformation and plant regeneration of the hardwood tree species Fraxinus profunda. Plant Cell Rep. 33:861-870.
Crossref

 
 

Sujatha M, Bahadur B, Reddy TP (2013). Interspecific hybridization in the genus Jatropha. In: Bahadur B, Sujatha M, Carels N (eds). Jatropha, Challenges for a New Energy Crop (V2): Genet. Improv. Biotechnol. Springer Sci. pp. 423-443.
Crossref

 
 

Tang W, Luo H, Newton RJ (2004). Effects of antibiotics on the elimination of Agrobacterium tumefaciens from loblolly pine (Pinus taeda) zygotic embryo explants and on transgenic plant regeneration. Plant Cell Tiss. Organ Cult. 79:71-81.
Crossref

 
 

Toppo DD, Singh G, Purshottam D, Misra P (2012). Improved in vitro rooting and acclimatization of Jatropha curcas plantlets. Biomass Bioenerg. 44:42-46.
Crossref

 
 

Tran TN, Mishra, NS (2015). Effect of antibiotics on callus regeneration during transformation of IR 64 rice. Biotechnol. Rep. 7:143-149.
Crossref

 
 

Varshney A, Johnson TS (2010). Efficient plant regeneration from immature embryo cultures of Jatropha curcas, a biodiesel plant. Plant Biotechnol. Rep. 4(2):139-148.
Crossref

 
 

Visarada K, Meena K, Aruna C, Srujana S, Saikishore N, Seetharama N (2009). Transgenic breeding: perspectives and prospects. Crop Sci. 49:1555-1563.
Crossref

 
 

Wani SP, Osman M, D'silva E, Sreedevi TK (2006). Improved livelihoods and environmental protection through biodiesel plantations in Asia. Asian Biotechnol. Develop. Rev. 8(2):11-29.

 
 

Wei Q, Lu WD, Liao Y, Pan SL, Xu Y, Tang L, Chen F (2004). Plant regeneration from epicotyl explant of Jatropha curcas. J. Plant Physiol. Mol. Biol. 30:475-478.

 
 

Xu J, Wang YZ, Xia Yin H, Jing Liu X (2009). Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system. Electron. J. Biotechnol. 12(1):3-4.
Crossref

 
 

Yong WTL, Abdullah JO, Mahmood M (2006). Optimization of Agrobacterium mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter. Sci. Hortic. 109:78-85.
Crossref

 
 

Yu TA, Yeh SD, Yang JS (2001). Effects of carbenicillin and cefotaxime on callus growth and somatic embryogenesis from adventitious roots of papaya. Bot. Bull. Acad. Sin. 42:281-286.

 
 

Zong H, Wang S, Ouyang C, Deng X, Li L, Li J, Chen F (2010). Agrobacterium-mediated transformation of Jatropha curcas young leaf explants with lateral shoot-inducing factor (LIF). Int. J. Agric. Biol. 12:891-896.