African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12513

Full Length Research Paper

Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers

Surendra Neupane
  • Surendra Neupane
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar
Jaishree Sijapati
  • Jaishree Sijapati
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar
Tribikram Bhattarai
  • Tribikram Bhattarai
  • Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Google Scholar
Sangita Shrestha
  • Sangita Shrestha
  • Molecular Biotechnology Unit, Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal
  • Google Scholar


  •  Received: 28 December 2016
  •  Accepted: 20 March 2017
  •  Published: 19 April 2017

References

Ahmmed SM, Mukherjee PK, Bahadur S, Harwansh RK, Kar A, Bandyopadhyay A, Al-Dhabi NA, Duraipandiyan V (2016). CYP450 mediated inhibition potential of Swertia chirata: An herb from Indian traditional medicine. J. Ethnopharmacol. 178:34-39.
Crossref

 

Bajpai MB, Asthana R, Sharma N, Chatterjee S, Mukherjee S (1991). Hypoglycemic effect of swerchirin from the hexane fraction of Swertia chirayita. Planta Med. 57(2):102-104.
Crossref

 
 

Balaraju K, Maheswaran R, Agastian P, Ignacimuthu S (2009). Egg hatchability and larvicidal activity of Swertia chirata Buch.-Hams. ex Wall. against Aedes aegypti L. and Culex quinquefasciatus Say. Indian J. Sci. Technol. 2(12):46-49.

 
 

Barakoti T (2002). Commercial Cultivation and Production Management of Chiraito: Scheme Guide. ARS Pakhribas, Nepal Agricultural Research Council, Nepal. Pp.1-50.

 
 

Barakoti T (2004). Attempts Made for Domestication, Conservation and Sustainable Development of Chiretta (Swertia chirayita). Nepal Agriculture Research Centre (NARC), Dhankuta.

 
 

Barakoti TP, Tiwari NN, Yonzon M (2013). Quality of Chiretta (Swertia chirayita) in Cultivated and Wild Samples Collected from Different Districts of Nepal. Nepal J. Sci. Technol. 13(2):57-62.
Crossref

 
 

Bornet B, Goraguer F, Joly G, Branchard M (2002). Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome 45(3):481-484.
Crossref

 
 

Botstein D, White RL, Skolnick M, Davis RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32(3):314.

 
 

Chen Y, Huang B, He J, Han L, Zhan Y, Wang Y (2011). In vitro and in vivo antioxidant effects of the ethanolic extract of Swertia chirayita. J. Ethnopharmacol. 136(2):309-315.
Crossref

 
 

Dudash MR (1990). Relative fitness of selfed and outcrossed progeny in a self-compatible, protandrous species, Sabatia angularis L.(Gentianaceae): a comparison in three environments. Evolution 44(5):1129-1139.
Crossref

 
 

Ghimire S (2008). Medicinal plants in the Nepal Himalaya: Current issues, sustainable harvesting, knowledge gaps and research priorities. Medicinal Plants in Nepal: an Analogy of Contemporary Research. Pp. 25-44.

 
 

Godt M, Hamrick J (1998). Allozyme diversity in the endangered pitcher plant Sarracenia rubra ssp. Alabamensis (Sarraceniaceae) and its close relative S. rubra ssp. rubra. Am. J. Bot. 85(6):802-802.
Crossref

 
 

Graham GC, Mayers P, Henry R (1994). A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. BioTechniques 16(1):48-50.

 
 

Hamrick J, Murawski DA (1991). Levels of allozyme diversity in populations of uncommon neotropical tree species. J. Trop. Ecol. 7(3):395-399.
Crossref

 
 

Hogbin PM, Peakall R (1999). Evaluation of the contribution of genetic research to the management of the endangered plant Zieria prostrata. Conserv. Biol. 13(3):514-522.
Crossref

 
 

Iqbal Z, Lateef M, Khan MN, Jabbar A, Akhtar MS (2006). Anthelmintic activity of Swertia chirata against gastrointestinal nematodes of sheep. Fitoterapia 77(6):463-465.
Crossref

 
 

Jaccard P (1908). Nouvelles recherches sur la distribution florale. Bull. Soc. Vard. Sci. Nat. 44:223-270.

 
 

Joshi K (2008). Swertia L.(Gentianaceae) in Nepal: Ethnobotany and Agenda for sustainable management. Ethnobot. Leafl. 12:1-6.

 
 

Joshi K (2011). Molecular Differentiation and Phylogeny of Swertia (Gentianaceae) of the Himalayan Region, Nepal. Int. J. Biochem. Biotechnol. 7(2):265-277.

 
 

Joshi P, Dhawan V (2005). Swertia chirayita-an overview. Curr. Sci. Bangalore 89(4):635

 
 

Joshi P, Dhawan V (2007). Analysis of genetic diversity among Swertia chirayita genotypes. Biol. Plant. 51(4):764-768
Crossref

 
 

Karak S, Nag G, De B (2017). Metabolic profile and β-glucuronidase inhibitory property of three species of Swertia. Rev. Bras. Farmacogn. 27(1):105-111.
Crossref

 
 

Kareem VA, Rajasekharan P, Ravish B, Mini S, Sane A, Kumar TV (2012). Analysis of genetic diversity in Acorus calamus populations in South and North East India using ISSR markers. Biochem. Syst. Ecol. 40156-40161

 
 

Khanal S, Shakya N, Thapa K, Pant DR (2015). Phytochemical investigation of crude methanol extracts of different species of Swertia from Nepal. BMC Res. Notes 8(1):821.
Crossref

 
 

Khoshoo T, Tandon S (1963). Cytological, morphological and pollination studies on some Himalayan species of Swertia. Caryologia 16(2): 445.

 
 

Kim S-C, Lee C, Santos-Guerra A (2005). Genetic analysis and conservation of the endangered Canary Island woody sow-thistle, Sonchus gandogeri (Asteraceae). J. Plant Res. 118(2):147-153.
Crossref

 
 

Kojima T, Nagaoka T, Noda K, Ogihara Y (1998). Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 96(1):37-45.
Crossref

 
 

Kshirsagar P, Gaikwad N, Pai S, Bapat V (2017). Optimization of extraction techniques and quantification of swertiamarin and mangiferin by using RP-UFLC method from eleven Swertia species. S. Afr. J. Bot. 10:881-889.
Crossref

 
 

Kshirsagar PR, Pai SR, Nimbalkar MS, Gaikwad NB (2016). RP-HPLC analysis of seco-iridoid glycoside swertiamarin from different Swertia species. Nat. Prod. Res. 30(7):865-868.
Crossref

 
 

Kulkarni R, Sreevalli Y, Baskaran K (2005). Allelic differences at two loci govern different mechanisms of intraflower self-pollination in self-pollinating strains of periwinkle. J. Hered. 96(1):71-77.
Crossref

 
 

Kumar V, Chandra S (2015). LC-ESI/MS determination of xanthone and secoiridoid glycosides from in vitro regenerated and in vivo Swertia chirayita. Physiol. Mol. Biol. Plants 21(1):51-60.
Crossref

 
 

Lad H, Bhatnagar D (2016). Amelioration of oxidative and inflammatory changes by Swertia chirayita leaves in experimental arthritis. Inflammopharmacology 24(6):363-375.
Crossref

 
 

Liu X-L, Qian Z-G, Liu F-H, Yang Y-W, Pu C-X (2011). Genetic diversity within and among populations of Neopicrorhiza scrophulariiflora (Scrophulariaceae) in China, an endangered medicinal plant. Biochem. Syst. Ecol. 39(4):297-301.
Crossref

 
 

Miller MP (1997). Tools for population genetic analyses (TFPGA) 1.3: A Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. 4:157.

 
 

Misra A, Shasany A, Shukla A, Darokar M, Singh S, Sundaresan V, Singh J, Bagchi G, Jain S, Saikia D (2010). AFLP markers for identification of Swertia species (Gentianaceae). Genet. Mol. Res. 9(3):1535-1544.
Crossref

 
 

Mondini L, Noorani A, Pagnotta MA (2009). Assessing plant genetic diversity by molecular tools. Diversity 1(1):19-35.
Crossref

 
 

Nagalekshmi R, Menon A, Chandrasekharan DK, Nair CKK (2011). Hepatoprotective activity of Andrographis paniculata and Swertia chirayita. Food Chem. Toxicol. 49(12):3367-3373.
Crossref

 
 

Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583-590.

 
 

Nepal I (2004). National register of medicinal & aromatic plants. IUCN Nepal Country Office for His Majesty's Government of Nepal, Ministry of Forests and Soil Conservation.

 
 

Nybom H (2004). Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol. Ecol. 13(5):1143-1155.
Crossref

 
 

Peakall R, Smouse PE (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28(19):2537-2539.
Crossref

 
 

Phoboo S, Jha P, Bhowmik P (2008). Biology and Phytochemistry of Swertia chirayita. Medicinal Plants in Nepal: Anthology of Contemporary Research, Ecological Society (ECOS), Kathmandu. Pp. 203-211.

 
 

Phoboo S, Pinto MDS, Barbosa ACL, Sarkar D, Bhowmik PC, Jha PK, Shetty K (2013). Phenolic‐Linked Biochemical Rationale for the Anti‐Diabetic Properties of Swertia chirayita (Roxb. ex Flem.) Karst. Phytother. Res. 27(2):227-235.
Crossref

 
 

Press JR, Shrestha KK, Sutton DA (2000). Annotated checklist of the flowering plants of Nepal. Natural History Museum Publications.

 
 

Prevost A, Wilkinson M (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet. 98(1):107-112.
Crossref

 
 

Proctor M, Yeo P, Lack A (1996). The natural history of pollination. HarperCollins Publishers.

 
 

Purohit K, Vijay B, Tiwari D, Tiwari A, Andola CH, Negi SJ, Chauhan SR (2013). Swertia chirayita (Roxb. ex Fleming) Karsten on the verge of extinction in the Himalayan region. Curr. Sci. 104(2):161.

 
 

Raina R, Patil P, Sharma YP, Rana RC (2013). Reproductive biology of Swertia chirayita–a temperate critically endangered medicinal plant. Caryologia 66(1):12-20.
Crossref

 
 

Ray S, Majumder HK, Chakravarty AK, Mukhopadhyay S, Gil RR, Cordell GA (1996). Amarogentin, a naturally occurring secoiridoid glycoside and a newly recognized inhibitor of topoisomerase I from Leishmania donovani. J. Nat. Prod. 59(1):27-29.
Crossref

 
 

Rijal DP (2010). Taxonomic study of some medicinally important species of Swertia L. (Gentianaceae) in Nepal. Botanica Orientalis 6:18-24.
Crossref

 
 

Saha P, Mandal S, Das A, Das PC, Das S (2004). Evaluation of the anticarcinogenic activity of Swertia chirata Buch. Ham, an Indian medicinal plant, on DMBA‐induced mouse skin carcinogenesis model. Phytother. Res. 18(5):373-378.
Crossref

 
 

Samaddar T, Than MMM, Jha TB, Jha S (2015). Cytogenetic and DNA fingerprinting analysis in three species of Swertia from Eastern Himalaya. Caryologia 68(3):207-216.
Crossref

 
 

Schaal BA, Leverich WJ, Rogstad SH (1991). Comparison of methods for assessing genetic variation in plant conservation biology. Genetics and conservation of rare plants.14:123-134.

 
 

Shah IA, Sharma Y, Raina R, Rana R (2011). Pollination studies in Swertia chirayita–a critically endangered medicinal plant of Western Himalayas. Open Access J. Med. Aromat. Plants 2(2):14-17.

 
 

Shrestha JC, Bhattarai T, Sijapati J, Rana N, Maharjan J, Rawal D, Raskoti B, Shrestha S (2013). Assessment of Genetic Diversity in Nepalese Populations of Swertia chirayita (Roxb. Ex Fleming) H. Karst Using RAPD-PCR Technique. Am. J. Plant Sci. 4(8):1617-1628.
Crossref

 
 

Shrestha N, Shrestha S, Koju L, Shrestha KK, Wang Z (2016). Medicinal plant diversity and traditional healing practices in eastern Nepal. J. Ethnopharmacol. 192:292-301.
Crossref

 
 

Shrestha S, Sijapati J, Rana N, Malla D, Regmi P, Raskoti B (2011). Optimization of RAPD-PCR conditions for the study of genetic diversity in Nepal's Swertia chirayita (Roxb. Ex Fleming) H. Karst. Himalayan J. Sci. 6(8):35-40.
Crossref

 
 

Slatkin M, Barton NH (1989). A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43(7):1349-1368.
Crossref

 
 

Soumendra C, Mukherjee D, Dasgupta T (2009). Cytological study on chromosome behaviour and new report on nature of mode of pollination of Swertia chirayita, a high value endangered medicinal plant of North Eastern Himalayan region. Caryologia 62(1):43-52.
Crossref

 
 

Tamhankar S, Ghate V, Raut A, Rajput B (2009). Molecular profiling of" Chirayat" complex using Inter Simple Sequence Repeat (ISSR) markers. Planta Med. 75(11):1266-1270.
Crossref

 
 

Tupe RS, Kemse NG, Khaire AA, Shaikh SA (2017). Attenuation of glycation-induced multiple protein modifications by Indian antidiabetic plant extracts. Pharm. Biol. 55(1):68-75.
Crossref

 
 

Wang B, Shi L, Ruan Z, Deng J (2011). Genetic diversity and differentiation in Dalbergia sissoo (Fabaceae) as revealed by RAPD. Genet. Mol. Res. 10:114-120.
Crossref

 
 

Wang HZ, Feng SG, Lu JJ, Shi NN, Liu JJ (2009). Phylogenetic study and molecular identification of 31 Dendrobium species using inter-simple sequence repeat (ISSR) markers. Sci. Hortic. 122(3):440-447.
Crossref

 
 

Wright S (1949). The genetical structure of populations. Ann. Eugen. 15(1):323-354.
Crossref

 
 

Yang L, Zhou G, Chen G (2011). Genetic diversity and population structure of Swertia tetraptera (Gentianaceae), an endemic species of Qinghai-Tibetan Plateau. Biochem. Syst. Ecol. 39(4):302-308.
Crossref

 
 

Zhang D, Chen S, Chen S, Zhang D, Gao Q (2007). Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau. Biochem. Genet. 45(1-2):33-50.
Crossref

 
 

Zhao WG, Zhang JQ, Wang YH, Chen TT, Yin YL, Huang YP, Pan YL, Yang YH (2006). Analysis of genetic diversity in wild populations of mulberry from western part of Northeast China determined by ISSR markers. J. Genet. Mol. Biol. 17(4):196-203.

 
 

Zhou NJ, Geng CA, Huang XY, Ma YB, Zhang XM, Wang JL, Chen JJ (2015). Anti-hepatitis B virus active constituents from Swertia chirayita. Fitoterapia 100:27-34.
Crossref