African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Overexpression of key enzymes of the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway for improving squalene production in Escherichia coli

Haiyuan Liu
  • Haiyuan Liu
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Shu Han
  • Shu Han
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Liping Xie
  • Liping Xie
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Jie Pan
  • Jie Pan
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Wei Zhang
  • Wei Zhang
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Guihua Gong
  • Guihua Gong
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar
Youjia Hu
  • Youjia Hu
  • China State Institute of Pharmaceutical Industry, Zhangjiang Insitute, Shanghai 201203, China.
  • Google Scholar


  •  Received: 07 September 2017
  •  Accepted: 16 November 2017
  •  Published: 13 December 2017

References

Ajikumar PK, Xiao WH, Tyo KEJ, Wang Y, Simeon F, Leonard E, Mucha O, Phon TH, Pfeifer B, Stephanopoulos G (2010). Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli. Science 330:70-74.
Crossref

 

Banerjee A, Sharkey TD (2014). Methylerythritol 4-phosphate (MEP) pathway metabolic regulation. Nat. Prod. Rep. 31(8):1043-1055.
Crossref

 
 

Du J, Yuan YB, Si T, Lian JZ, Zhao HM (2012). Customized optimization of metabolic pathways by combinatorial transcriptional engineering. Nucleic Acids Res. 40(18):e142.
Crossref

 
 

Ghimire GP, Lee HC, Sohng JK (2009). Improved squalene production via modulation of the methylerythritol 4-phosphate pathway and heterologous expression of genes from Streptomyces peucetius ATCC 27952 in Escherichia coli. Appl. Environ. Microbiol. 75(22):7291-7293.
Crossref

 
 

Ghimire GP, Thuan NH, Koirala N, Sohng JK (2016). Advances in biochemistry and microbial production of squalene and its derivatives. J. Microbiol. Biotechnol. 26(3):441-451.
Crossref

 
 

Han GH, Kim SK, Yoon PK, Kang Y, Kim BS, Fu Y, Sung BH, Jung HC, Lee DH, Kim SW, Lee SG (2016). Fermentative production and direct extraction of (-)-alpha-bisabolol in metabolically engineered Escherichia coli. Microb. Cell Fact. 15(1):185.
Crossref

 
 

Hien HTM, Ha NC, Thom LT, Hong DD (2017). Squalene promotes cholesterol homeostasis in macrophage and hepatocyte cells via activation of liver X receptor (LXR) α and β. Biotechnol. Lett. 39(8):1101-1107.
Crossref

 
 

Jiang M, Stephanopoulos G, Pfeifer BA (2012). Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds. Appl. Environ. Microbiol. 78(8):2497-2504.
Crossref

 
 

Katabami A, Li L, Iwasaki M, Furubayashi M, Saito K, Umeno D (2015). Production of squalene by squalene synthases and their truncated mutants in Escherichia coli. J. Biosci. Bioeng. 119(2):165-171.
Crossref

 
 

Kim J, Copley SD (2012). Inhibitory cross-talk upon introduction of a new metabolic pathway into an existing metabolic network. PNAS. 109(42):E2856-E2864.
Crossref

 
 

Kotelevets L, Chastre E, Caron J, Mougin J, Bastian G, Pineau A, Walker F, Lehy T, Desmaele D, Couvreur P (2017). A Squalene-Based Nanomedicine for Oral Treatment of Colon Cancer. Cancer Res. 77(11):2964-2975.
Crossref

 
 

Kumar SR, Yamauchi I, Narayan B, Katsuki A, Hosokawa M, Miyashita K (2016). Squalene modulates fatty acid metabolism: Enhanced EPA/DHA in obese/diabetic mice (KK-Ay) model. Eur. J. Lipid Sci. Technol. 118(12):1935-1941.
Crossref

 
 

Leonard E, Ajikumar PK, Thayer K, Xiao WH, Mo JD, Tidor B, Stephanopoulos G, Prather KLJ (2010). Combining metabolic and protein engineering of a terpenoid biosynthetic pathway forcoverproduction and selectivity control. PNAS. 107(31):13654-13659.
Crossref

 
 

Liu HW, Wang Y, Tang Q, Kong WT, Chung WJ, Lu T (2014). MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli. Microb. Cell Fact. 13:135.
Crossref

 
 

Lv XM, Gu J, Wang F, Xie WP, Liu M, Ye LD, Yu HW (2016). Combinatorial Pathway Optimization in Escherichia coli by Directed Co-Evolution of Rate-Limiting Enzymes and Modular Pathway Engineering. Biotechnol. Bioeng. 113(12):2661-2669.
Crossref

 
 

Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD (2003). Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nature Biotechnology. 21(7):796-802.
Crossref

 
 

Meng HL, Wang Y, Hua Q, Zhang SL, Wang XN (2011). In silico analysis and experimental improvement of taxadiene heterologous biosynthesis in Escherichia coli. Biotechnology and Bioprocess Engineering. 16(2):205-215.
Crossref

 
 

Sambrook J, Russell DW (2001). Molecular cloning: A laboratory manual. 3rd edition. New York:Cold Spring Harbor laboratory Press, Cold Spring Harbor.

 
 

Weaver LJ, Sousa MML, Wang G, Baidoo E, Petzold CJ, Keasling JD (2015). A kinetic-based pproach to understanding heterologous mevalonate pathway function in E. coli. Biotechnol. Bioeng. 112:111-119.
Crossref

 
 

Xue J, Ahring BK (2011). Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis. Appl. Environ. Microbiol. 77(7): 2399-2405.
Crossref

 
 

Yuan LZ, Rouviere PE, Larossa RA, Suh W (2006). Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab. Eng. 8(1):79-90.
Crossref

 
 

Zhao J, Li Q, Sun T, Zhu X, Xu H, Tang J, Zhang X, Ma Y (2013). Engineering central metabolic modules of Escherichia coli for improving beta-carotene production. Metab. Eng. 17: 42-50.
Crossref

 
 

Zhao J, Liu Y, Li Q, Zhu X, Zhang X (2013). Modulation of isoprenoid gene expression with multiple regulatory parts for improved beta-carotene production. Chin. J. Biotech. 29(1):41-55.

 
 

Zheng YN, Liu Q, Li LL, Qin W, Yang JM, Zhang HB, Jiang XL, Cheng T, Liu W, Xu X, Xian M (2013). Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels. Biotechnology for Biofuels. 6:57.
Crossref

 
 

Zhou K, Zou RY, Stephanopoulos G, Too HP (2012). Metabolite profiling identified methylerythritol cyclodiphosphate efflux as a limiting step in microbial isoprenoid production. PLOS ONE. 7(11):e47513.
Crossref