African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 10 May 2010; 9(19)

May 2010

Screening of resistance genes to fusarium root rot and fusarium wilt diseases in F3 family lines of tomato (Lycopersicon esculentum) using RAPD and CAPs markers

  Fusarium diseases constitute most of the loss in tomato production worldwide, because it spreads on all geographic fields that it is so hard to find a place without fusarium infestation. Thus, the best way to produce tomato is developing resistant cultivars against Fusarium species. In cultivar developing, molecular marker assisted techniques replaced traditional breeding techniques which are high cost...

Author(s): Bahattin Tanyolaç and Cengiz Akkale

May 2010

Genetic variability, heritability and genetic advance of quantitative traits in black gram by effects of mutation in field trail

  Genetic variation has led to an increase in the quantitative traits of crops. The variability on genome is induced by mutation, which enhances the productivity. We evaluated variability on quantitative characters such as, plant height, number of branches/plant, number of leaves/plant, number of fruit clusters/plant, number of pods/plant, number of seeds/pod, yield/plant and 100 seed weight of black gram in...

Author(s): D. Arulbalachandran, L. Mullainathan, S. Velu and C. Thilagavathi

May 2010

Development of male sterility by silencing Bcp1 gene of Arabidopsis through RNA interference

  The development of male sterility is one of the most important steps in hybrid seed production. Several methods for the abortion of pollens based on conventional as well as genetic engineering are reported for the various crop species. Here we have investigated the use of RNA interference (RNAi) technology to silence a male specific gene, Bcp1 in the model host Arabidopsis...

Author(s): Muhammad Tehseen, Muhammad Imran, Mazhar Hussain, Shazia Irum, Shahid Ali, Shahid Mansoor and Yusuf Zafar

May 2010

Function analysis of two Mn(II) ion transporter genes (DR1709 and DR2523) in Deinococcus radiodurans

  Deinococcus radiodurans is best known for its extraordinary radiation resistance. Manganese(II) ions can protect proteins from being damaged by super radicals, which is supposed to be the main mechanism through which D. radiodurans has the strong radiation resistance. But the detailed molecular and physiological processes about how Mn(II) ions are transported in this bacterium is still not...

Author(s): Shu Haiyan and Tian Baoming

May 2010

The expression of VFL and VvTFL1 genes in relation to the effects of gibberellins in different organs of “Xiangfei” grapevine

  The effects of gibberellins (GAs) on plant growth and the expression of VFL andVvTFL1 genes in different organs of “Xiangfei” grapevine (Vitis vinifera L.) were investigated. The endogenous GA content significantly increased in the apical bud,inflorescence and tendril after GA treatment of grapevine. The expression of theVFL gene was obviously reduced in the meristem...

Author(s): Anyan Yao, Yuyan Yang, Kang Liao, Lusheng Zhang and Jianfang Hu

May 2010

Evaluation of various substrates and supplements for biological efficiency of Pleurotus sajor-caju and Pleurotus ostreatus

  An experiment was conducted to determine the effects of different substrates namely wheat straw (Triticum aestivum), maize stover (Zea mays L), thatch grass (Hyparrhenia filipendula) and oil/protein rich supplements (maize bran, cottonseed hull [Gossypium hirsutum]) on biological efficiency of two oyster mushroom species (Pleurotus sajor-caju and P. ostreatus). Wheat straw had...

Author(s): M. Fanadzo, D. T. Zireva, E. Dube and A. B. Mashingaidze

May 2010

Preparation of activated carbon from a renewable agricultural residue of pruning mulberry shoot

  In this study, element composition of pruning mulberry shoot was evaluated by element analysis. On the basis of this, pruning mulberry shoot–based activated carbon was prepared from by chemical activation with phosphoric acid (H3PO4), which particles were displayed by the scanning electron microscope (SEM) micrographs. The influence of impregnation temperature, impregnation...

Author(s): Jun Wang, Fu-An Wu, Meng Wang, Ning Qiu, Yao Liang, Shui-Qin Fang and Xing Jiang

May 2010

Adventitious shoot regeneration from leaf explants of miniature paprika (Capsicum annuum) ‘Hivita Red’ and ‘Hivita Yellow’

  A simple and efficient protocol was developed for in vitro propagation of two miniature paprika cultivars. Seeds of miniature paprika (Capsicum annuum) ‘Hivita Red’ and ‘Hivita Yellow’ were decontaminated and placed in a petri dish containing a half-strength MS medium and then were incubated in the dark for 7 10 days for germination. Leaf explants excised...

Author(s): Ju Yeon Song, Iyyakkannu Sivanesan, Chul Geon An and Byoung Ryong Jeong,

May 2010

Culture-independent analysis of microflora in Gayals (Bos frontalis) feces

  Comparative DNA sequence analysis of 16S/18S rRNA genes (rDNA) were undertaken to further our understanding of the make-up of micro-organisms communities in the feces of Gayals. Total DNA were extracted from the feces of 5 Gayals. Two rDNA libraries (16S/18S rDNA) were constructed. In the 16S rDNA library, phylogenetic and sequence similarity analyses of the resultant 71 clone sequences revealed the presence...

Author(s): Gao Run-chi, Huang Zun-xi, Wu Chang-fei,, Xu Bo, and Wang Xiao-yan,

May 2010

Active substance from some blue green algal species used as antimicrobial agents

  The concept of biological control for health maintenance has received widespread attention during the last few years. Therefore, the main objective of this work was to look for active substances that could be used as antimicrobial agents in an efficient and safe manner. To achieve this target, five different extracts (ethyl acetate, chloroform, diethyl ether, methanol and water) from three...

Author(s): Hanan M. Khairy and Hala Y. El-Kassas

May 2010

Bioleaching of metal ions from low grade sulphide ore: Process optimization by using orthogonal experimental array design

  The present work was aimed at studying the bioleachability of metal ions from low grade sulphide ore containing high amount of carbonaceous materials by selected moderately thermophilic strain of acidophilic chemolothotrophic bacteria,Sulfobacilllus thermosulfidooxidans. The bioleaching process was optimized by constructing L25 Taguchi orthogonal experimental array design and optimization of variable...

Author(s): Sadia Ilyas, Haq Nawaz Bhatti, Ijaz Ahmad Bhatti, Munir Ahmad Sheikhand Muhammad Afzal Ghauri

May 2010

A semi-automatic system for labelling seafood products and obtaining fishery management data: A case study of the bottom trawl fishery in the central Mediterranean Sea

  This study is on the implementation of a semi-automatic labelling system (LS) of the Mediterranean Sea seafood harvest to address the increased need for seafood authentication and inherent difficulties of commonly used indirect techniques for estimating fisheries yield and fishing effort. Sensitive data required for anti-counterfeit policies, such as date and catch area, can be acquired and recorded on the...

Author(s): Gioacchino Bono, Salvatore Cusumano, Cinzia Badalucco, Vito Pipitone and Sergio Vitale

May 2010

Chitosan activates defense responses and triterpenoid production in cell suspension cultures of Betula platyphylla Suk

  Triterpenoid production and plant defense enzyme activity in suspension cultures ofBetula platyphylla Suk. cells treated with chitosan elicitor were investigated.Chitosan, at the optimal concentration of 100 mgL-1 enhanced triterpenoid production by 2.6-fold, with a maximum yield of 202.75 versus 78.72 mgL-1 of the untreated control cells. Furthermore, the activities of...

Author(s): Guizhi Fan, Xiaocan Li, Xiaodong Wang, Qiaoli Zhai and Yaguang Zhan

May 2010

Utilization of sun-dried on-farm generated poultry litter as a feed resource for growing-finishing pigs

  Forty eight (48) growing-finishing pigs (36.11 ± 1.26 kg) were allotted to three dietary treatment groups of 0, 33.33 and 66.67% sun-dried on-farm generated poultry litter (SOPL) as a replacement for 30% maize in diets of growing-finishing pigs on weight basis to assess its implication on the chemical composition of the feed, growth performance and blood chemistry of the pigs. Each treatment was...

Author(s):   A. O. K. Adesehinwa, O. O. Obi, B. A. Makanjuola, A. O. Adebayo and E. S. Durotoye