July 2011
Glutathione, cell proliferation and differentiation
All organisms require an equivalent source for living. Reduced glutathione is the most abundant thiol containing protein in mammalian cells and organs. Glutathione was discovered by Hopkins in 1924 who published his findings in JBC. It is a three peptide containing glutamic acid, cystein and glycin and is found in reduced and oxide forms in cell. High concentration of glutathione and its high reduced/oxide...
July 2011
Genetic polymorphisms of 17 Y-chromosome STRs in Han population from Henan province of China
Haplotypes and allele frequencies of 17 Y-short tandem repeats (STRs) included in the PowerPlex1 Y systems kit (DYS19, DYS389 I, DYS389 II, DYS390,DYS391, DYS392, DYS393, DYS385, DYS438, DYS439, DYS437, DYS448, DYS456, DYS458, DYS635 and Y GATA H4) were examined in a population sample of 276 unrelated Han male living in Henan province. A total of 272 haplotypes were observed in the 276 individuals...
July 2011
TNF-α expression, not iNOS expression, is correlated with NF-κB activation in the spinal cord of rats following peripheral nerve injury
Previous studies have shown that pro-inflammatory cytokines were involved in the genesis and persistence of neuropathic pain. Nuclear factor kappa B (NF-κB) plays a crucial role in regulating pro-inflammatory cytokine gene expression. In this study, we examined the hypothesis that NF-κB would regulate the expression of spinal tumor necrosis factor α (TNF-α) and inducible nitric...
July 2011
Amplified fragment length polymorphism (AFLP) studies on Indian Cycas species
Amplified fragment length polymorphism (AFLP) technology was used to reveal the genetic variation in six species of Cycas collected from eleven natural populations. Two sets of primer with 4-selective nucleotides were used in this study and 78% polymorphism was found. The results correlated with geographical distribution of the plants and grouped into four. This study is highly useful...
July 2011
Amplified fragment length polymorphisms (AFLPs) analysis of species of Solanum section Solanum (Solanaceae) from Uganda
The taxonomy of species belonging to Solanum section Solanum (sometimes referred to as the Solanum nigrum complex or black nightshades) is known to be difficult and has resulted in extensive synonymy. Yet, these species play a significant role in nutrition and food security, especially in developing countries. The amplified fragment length polymorphisms (AFLPs) technique was used...
July 2011
A nonsense (c.3978G>A) abnormal spindle-like, microcephaly associated (ASPM) gene mutation is a major cause of primary microcephaly in Pashtoon ethnic group of Pakistan
Primary microcephaly (MCPH) is an autosomal-recessive congenital disorder characterized by smaller-than-normal brain size and mental retardation. MCPH is genetically heterogeneous with six known loci: MCPH1 to MCPH7. The abnormal spindle-like, microcephaly associated (ASPM) gene at MCPH5 locus, which accounts for 37 to 54% of MCPH, appears to be the most common cause of microcephaly. More than 50% of the MCPH...
July 2011
Comparative analysis of transformed potato microtubers and its non-transformed counterpart using some biochemical analysis along with inter simple sequence repeat (ISSR) marker
The rapid progress of transgenic biotechnology has significantly promoted the development and production of genetically modified (GM) crops. The aim of this study was to compare some compositional analysis and genetic variation of transformed potato microtubers (Solanum tuberosum L. Desiree) line (which harbor potato virus Y coat protein gene) with its conventional line in order to understand the...
July 2011
Preliminary research of recombinant matrix extracellular phosphoglycoprotein (MEPE) mineralization ability in vitro
The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone and teeth mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE after cloning its cDNA from the cDNA library of a human brain cDNA...
July 2011
Genome scanning for identification of resistance gene analogs (RGAs) in a highly durable blast resistance rice (Oryza sativa L.) cultivar, Moroberekan
Disease resistance in plants is a desirable economic trait. Many disease resistance genes from various plants have been cloned so far. The gene products of some of these can be distinguished by the presence of an N terminal nucleotide binding site and a C-terminal stretch of leucine-rich repeats. Oligonucleotides already designed from sequence motifs conserved between resistance N genes of tobacco...
July 2011
Optimization of somatic embryogenesis induction in Iranian melon (Cucumis melo cv. Khatooni)
In this study, regeneration of Cucumis melo cv. Khatooni: one specific Iranian melon- via somatic embryogenesis was investigated. Three different types of explant (cotyledon, hypocotyl and cotyledonary petiole) from six days old seedlings were used. Growth regulator treatments were two levels of 6-benzyl aminopurine (0 and 0.1 mg/l) and four levels of 2,4-dichlorophenoxyacetic acid (0, 2.5, 5 and...
July 2011
Chitosan application in maize (Zea mays) to counteract the effects of abiotic stress at seedling level
Worldwide, the conditions of biotic and abiotic stresses adversely affect the potential production of maize. Drought or heat facilitate the infection with fungi such as Aspergillus flavus and Fusarium moniliforme, and consequently increase the production of mycotoxins. There are several strategies for managing the problem, but in the future, people will prefer the cleaner and cheaper...
July 2011
Establishment of high effective regeneration and propagation system for ornamental crabapple (Malus spp.)
In order to establish efficient regeneration system for ornamental tissue culture, we used Malus spp. ‘Indian Magic as the experimental materials and investigated the effects of disinfection and anti-browning agents, culture mediums and hormones proportion on differentiation, multiplication, callus induction and rooting, and also the effects of culture substrates on growth of transplants...
July 2011
Rice field for the treatment of pond aquaculture effluents
We conducted an experiment to evaluate the efficiency of rice fields in treating pond aquaculture effluent and its responses to different fertilizer treatments. Four treatments was considered in the experiment: no rice planted as the control (CT); rice planted and no fertilizer input (RE); rice planted and a rate of approximately 1.0 g...
July 2011
Water and nitrogen interactively increased the biomass production of Jerusalem artichoke (Helianthus tuberosus L.) in semi-arid area
The response of height, aboveground and underground biomass of Jerusalem artichoke (Helianthus tuberosus L.) to water and nitrogen fertilization were studied in north-eastern Inner Mongolia, China. The results showed that water was a main limiting factor on the height and yield of Jerusalem artichoke; it significantly improved the height and yield of Jerusalem artichoke (including leaf, stem,...
July 2011
Detection of species diversity of arbuscular mycorrhizal fungi (AMF) classic and molecular methods from the melon (Cucumis melo L.) plants in Van and its districts
Arbuscular-mycorhizal fungi (AMF) from melon plants grown in Van province, were studied by nested-PCR method to establish colonization ratio of related fungi in plants and to detect the fungi at species level. From 10 different locations, a total of 100 soil samples were taken from rhizosphere area of melon plants. It was also determined that 52% of these plants were colonized by arbuscular-mycorhizal fungi...
July 2011
Glucosinolate content and related gene expression in response to enhanced UV-B radiation in Arabidopsis
Increasing UV-B radiation reaching the earth's surface can affect the growth and development of plants. Glucosinolate metabolism is evolved through plant interactions with the environment and constantly regulated by different environmental factors. We investigated the contents of glucosinolates and the expression of related genes in response to enhanced UV-B radiation (1.55 W·m-2) and the...
July 2011
Herbicidal effects of aqueous extracts of three Chenopodium species on Avena fatua
Herbicidal effects of aqueous leaf extracts of three Chenopodium species;Chenopodium album L., Chenopodium murale L. and Chenopodium ambrosioideswere evaluated on wild oat (Avena fatua L.), one of the problematic weeds of wheat. Among the aqueous extracts of 0, 2, 4 and 6% (w/v) employed in bioassays, 6% ofC. album and 2% of C. murale caused significant...
July 2011
Effects of different irrigation programs on yield and quality parameters of eggplant (Solanum melongena L.) under greenhouse conditions
This study was carried out to determine the effects of different irrigation programs on yield and quality parameters of eggplant under greenhouse conditions, using Class A pan evaporation calculations and different plant-pan coefficients. Irrigation water was applied through drip irrigation method twice a week during the growing period. Irrigation treatments consisted of five plant-pan coefficients (S1:...
July 2011
Effect of water stress on yield and yield components of sunflower hybrids
A field experiment during year 2009 was conducted in the research station of the University of Tehran, College of Abouraihan in Pakdasht region, Iran. The study was aimed to investigate the effect of water stress on seed yield, yield component and some quantitative traits of four sunflower hybrids namely Azargol, Alstar, Hysun 33 and Hysun 25 under three different irrigation regimes; irrigation...
July 2011
Effects of olive oil solid waste on growth and nutrient uptake of faba bean, onion, and radish plants
Although, olive oil industry is crucial for Turkey, olive oil solid waste or olive vegetation water, a by-product of the industry, leads to environmental pollution during olive oil production. This study investigated the fields where olive oil solid waste was used as an organic substance. Olive oil solid waste in different concentrations (0, 10, 20, 30 and 40 t ha-1) were applied to...
July 2011
Challenges in commercialization of nano and biotechnologies in agricultural sector of Iran
The major purpose of this study was to determine challenges in commercialization of nano and biotechnologies in agicultural sector of Iran. The total population for this study was 50 participants who attended a workshop on commercialization of nano and biotechnologies in agriculture at biotech 2010 exhibition in Tehran. The results showed that the social and cultural challenges caused 39% of...
July 2011
The efficacy of endogenous gibberellic acid for parthenocarpy in eggplant (Solanum melongena L.)
Eggplants are generally grown by winter in Mediterranean areas. Therefore, growers prefer to use parthenocarpic fruit and plant growth regulators. This study determined the relationship between flower development and gibberellic acid (GA3) levels in parthenocarpic and non-parthenocarpic eggplant (Solanum melongena L.) genotypes. A single crop was grown in an unheated greenhouse at the Bati...
July 2011
Identification and pathogenicity assessment of Fusarium spp. sampled from durum wheat fields in Tunisia
Durum wheat is the major cereal crop cultivated in Tunisia; covering over 40% of the cereal growing areas. Durum wheat production remains below expectation due to its low productivity that is attributed to the chronically abiotic and biotic stresses. Fusarium head blight (FHB) caused by Fusarium spp. has become an important disease on durum wheat (Triticum turgidum L var. durum)...
July 2011
Growth rate of Scenedesmus acutus in laboratory cultures exposed to diazinon
The effects of the pesticide, diazinon on growth rate was examined in unialgal cultures of freshwater green algae, Scenedesmus acutus through 96 h acute toxicity tests. S. acutus was exposed to different concentrations of diazinon (1, 2, 4, 8, 16 and 32 µl) in the laboratory maintained at 23 ± 1°C and 16:8 light : dark regime. Cell numbers were determined daily and growth...
July 2011
Association of the Glu298→Asp polymorphism in the endothelial nitric oxide synthase gene with risk of coronary artery disease
Genetic variants of endothelial nitric oxide synthase (eNOS) could influence individual susceptibility to coronary artery disease (CAD) with or without associated demographic factors. The aim of this study was to assess whether Glu298/Asp polymorphism of the eNOS gene is associated with the occurrence and severity of angiographycially defined coronary artery disease. Polymerase chain reaction/restriction...
July 2011
Screening, identification and degrading gene assignment of a chrysene-degrading strain
A predominant chrysene-degrading strain named CT was isolated from the activated sludge of Zhenjiang coking plant. The strain was initially identified asParacoccus aminovorans by the results of morphological observation, physio-biochemical test and 16S rDNA gene sequence analysis. Under the conditions of initial chrysene concentration of 40 mg/l, inoculation amount of 10% (V/V) at pH 7.0 and...
July 2011
Exobiopolymer from polyhydroxyalkanoate-producing transgenic yeast
Recently, the wild type yeast Kloeckera sp. strain KY1 was equipped in their cytoplasm with the phaABC operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha. Unpredicted, resulted transgenic yeast strain KY1/PHA was able to synthesize another exopolymer beside the production of PHA. Subsequently, produced exopolymer was subject for further...
July 2011
Simultaneous production of raw starch degrading highly thermostable a-amylase and lactic acid by Lactobacillus fermentum 04BBA19
The widely used thermostable amylases were produced long time ago fromBacillus genus. Although, lactic acid bacteria (LAB) fermentation presents several advantages including the reduction of growth of pathogenic microorganisms, no study has yet reported thermostable amylases from lactic acid bacteria. An amylolytic LAB, Lactobacillus fermentum (04BBA19) isolated from starchy wastes of a...
July 2011
Correlations among sensory characteristics and relationships between aroma scores, flavour scores, off-flavour scores and off-flavour descriptors of chevon from four goat genotypes
The objective of the current study was to determine the correlations among sensory characteristics and relationships between flavour scores and off-flavour descriptors of chevon. Forty-eight male 6-months old Xhosa lop-eared, Nguni, Xhosa-Boer cross and Boer goat kids were kept at the University of Fort Hare Farm until slaughter. Sample cuttings for meat tasting were made from the hind leg. Aroma score was...
July 2011
Effect of 1-methylcyclopropene and modified atmosphere packaging on chilling injury and antioxidative defensive mechanism of sweet pepper
Some sweet peppers (Capsicum annuum L.) are chilling sensitive and can develop injury when stored at temperatures less than 7°C. This study was conducted to investigate the effect of 1-methylcyclopropene (1-MCP) (650 ppb) and modified atmosphere packaging (MAP) on chilling injuries (CI) of sweet pepper during 30 days storage at 4°C. The results showed that, 1-MCP and MAP reduced chilling injury...
July 2011
Immobilization of urease on copper chelated EC-Tri beads and reversible adsorption
In the present study, Eupergit C® macroporous beads were functionalized with amino triazole and characterized by FTIR-ATR and SEM. Cu2+ ions were chelated on the triazole modified Eupergit C® (EC®), and then the metal chelated beads were used in the adsorption of urease. Maximum reaction rate (Vmax) and Michaelis-Menten constant (km) were determined for the free and immobilized...
July 2011
Phytochemical investigation and anti-inflammatory property of ethanol-water extract of the roots of Anthocleista djalonensis A. Chev. (Gentianiaceae)
Anthocleista djalonensis A.Chev. (Gentianiaceae) a plant of West African origin is used for the treatment of inflammatory disorders traditionally. Phytochemical screening of the plant root material gave positive result for tannins, saponins and carbohydrate; but negative for alkaloids and flavonoids. The ethanol-water (1:1) extract of the root material was subjected to anti-inflammatory activity using...
July 2011
Prevalence of Chlamydia trachomatis infections in symptomatic women by polymerase chain reaction (PCR) immunofluorescence and Giemsa stain
Chlamydia trachomatis is a ubiquitous human pathogen that is responsible for the most prevalent bacterial sexually transmitted disease worldwide. Studies show that polymerase chain reaction (PCR) is more sensitive than cellular culture for detection of C. trachomatis infections. The aim of this study is to compare different laboratory methods, including Giemsa staining, direct...
July 2011
Antioxidative potential of polysaccharide fractions produced from traditional Chinese medicinal macrofungus Cordyceps jiangxiensis in vitro
Cordyceps jiangxiensis, also called ‘CaoMuWang’, is a medicinal entomopathogenic macrofungus native to eastern China. Polysaccharide fractions from cultured C. jiangxiensis exhibited potent antitumor activity via the induction of cell cycle arrest and apoptotic pathway. Antioxidant pathway is also one action of mechanism of antitumor; thus, the antioxidant abilities of these...
July 2011
Prevalence and detection of cytomegalovirus by polymerase chain reaction (PCR) and simple ELISA in pregnant women
A total of 327 women were screened, amongst them, 7 (2.14%) werecytomegalovirus (CMV) DNA positive by polymerase chain reaction (PCR). Antibodies against toxoplasma were also detected in 106 (32.41%) women, while 54 (16.51%) were anti CMV positive. Eleven (3.36%) and thirteen (3.97%) women were anti HSV and anti Rubella virus positive, respectively. High prevalence was recorded in age group 21 to 30...
July 2011
Bioactivity of essential oil from Satureja hortensis (Laminaceae) against three stored-product insect species
Human health problems and environmental hazards caused by the indiscriminate use of chemical pesticides during the past three decades, led scientists to look for less persistent and biodegradable alternatives. Essential oils from aromatic plants are recognized as proper alternatives. In this experiment, toxicity of Satureja hortensis essential oil that was isolated via hydrodistillation was...
July 2011
Seasonal variation of trace elements in muscle tissues of two commercially valuable freshwater fish species (Silurus triostegus and Barbus grypus Heckel, 1843) from Atatürk Dam Lake (Turkey)
In this study, some trace metals (Pb, Cd, Hg, Cu, Zn and Fe) were seasonally determined in the muscle tissues of two commercially valuable freshwater fish species (Silurus triostegus and Barbus grypus, Heckel, 1843) from Atatürk Dam Lake. The obtained results showed that the average values of Fe (9.816 ± 0.390 μg/g) and Cu (1.343 ± 0.0.589 μg/g) in B. grypus were...
July 2011
Structural modeling of natural citrus products as potential cross-strain inhibitors of Dengue virus
There are four serotypes of Dengue virus and there are existing drugs used against specific serotype. There is no drug that is effective against all strains of this virus. In this research, bioinformatics tools were used to predict the affinity of natural ligands for the glycoprotein E of Dengue virus by considering the conserved domains. Molecular docking studies were carried out by using Autodock 3.0....
July 2011
Measurement of oxygen consumption rate of osteoblasts from Sprague-Dawley rat calvaria in different in vitro cultures
The oxygen consumption rates of Sprague-Dawley (SD) rat’s osteoblasts cultured in different in vitro media were on-line measured by using precision dissolved oxygen and respirometry instrumentation. The osteoblasts, isolated from the calvaria of neonatal SD rat by an enzymatic digestive process of 0.25% trypsin solution and 0.1% type II collagenase, were diluted to 1 × 106 cells/ml...
July 2011
A new taxol-producing fungus (Pestalotiopsis malicola) and evidence for taxol as a transient product in the culture
Fungal production of the anti-tumor taxol is an effective way of making this drug in industries. We reported here a new taxol-producing fungus, NK101, from plant debris in the soil. Based on the culture characteristics, conidia structure and molecular evidence, NK101 was classified as Pestalotiopsis malicola. Taxol was verified in both the culture and the mycelium in a high level (186 μg/L). The...
July 2011
Effects of supplemental microbial phytase enzyme on performance and phytate phosphorus digestibility of a corn-wheat-soybean meal diet in broiler chicks
This experiment was conducted to investigate the effects of supplemental phytase in a corn-wheat-soybean meal basal diet on phosphorus (P) digestibility and performance of broiler chicks. 378 one-day old broiler chicks (Ross 308) were allocated to 3´3 factorial arrangements with three levels of phytase enzyme (0, 500 and 1000 FTU/kg) and three levels of non-phytate P (100, 80 and 60% of NRC...
July 2011
Fattening performance, blood parameters and slaughter traits of Karya lambs consuming blend of essential oil compounds
The effects of feeds containing a blend of essential oil compounds on fattening performance, some blood parameters, the slaughter traits, and internal organ weights of Karya lambs were researched. The lambs were divided into 2 treatment groups: the first group (control, C) consumed mixed feed without blend of essential oil compounds, while the other group (BEOC) consumed mixed feed with blend of essential oil...
July 2011
Protective effect of melatonin on sperm motility parameters on liquid storage of ram semen at 5°C
Oxidative damage to sperm resulting from reactive oxygen species generated by the cellular components of semen during liquid storage is possibly one of the main causes of the decline in motility during storage. The aim of this study was to determine the effects of melatonin as an antioxidant on sperm motility parameters in liquid storage of ram semen at 5°C. A total number of 24 ejaculates were collected...
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