African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12405

Table of Content: 12 Apr 2012; 11(30)

April 2012

Molecular cloning and characteristic analysis of a thioredoxin from Neobenedenia melleni

  Thioredoxin (Trx) can regulate disulfide bond reduction of target proteins to maintain the reduced intracellular state in various organisms. Here, we cloned a cDNA sequence of thioredoxin from Neobenedenia melleni, which is a kind of platyhelminth parasite infecting many fishes of great economic value. The deduced N. melleni Trx (NmTrx) contained 170 amino acid residues with an active site...

Author(s):   Zhe-Liang Sheng, Xin-Jiang Lu and Jiong Chen  

April 2012

Ultra-structural study of Egyptian Buffalo oocytes before and after in vitro maturation

  The purpose of this study was to describe the changes occurring in the cytoplasmic organelles of the buffalo oocytes before and after in vitro maturation. The total number of oocytes used in this experiment was 250 oocytes; 50 in vivo matured oocytes, 100 immature oocytes, and the other 100 was in vitro matured oocytes cultured in TCM-199 + LH. The oocytes examined in this study...

Author(s):   I. A. H. Barakat, H. M. El–Ashmaoui, A. Barkawi, S. A. Kandeal, and E. EL-Nahass  

April 2012

Optimization of plasmid electrotransformation into Escherichia coli using Taguchi statistical method

  Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane. In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer allowing molecules like DNA to pass into the cell. Application of statistical methods to determine the appropriate processes have been suggested for genetic engineering and biotechnology technique such as...

Author(s):   Mohamad Heiat, Hossein Aghamollaei, Seeyed mostafa Hoseinei, Reza Abbasi Larki and Kheirollah Yari  

April 2012

Isolation and identification of Metarhizium anisopliae from Chilo venosatus (Lepidoptera: Pyralidae) cadaver

  Sugarcane stem borer, Chilo venosatus Walker (Lepidoptera: Pyralidae) is a significant sugarcane pest in South China. Conidia or mycelia collected from the surface of sugarcane stem borer cadavers were cultured. The colony morphology, mycelia and conidial yield were observed with three-agar culture media: potato dextrose agar medium (PDA), potato dextrose with 1% (w/v) peptone agar medium...

Author(s):   Lei Liu, Rulin Zhan, Laying Yang, Changcong Liang, Di Zeng and Junsheng Huang        

April 2012

Purification of an elicitor from Magnaporthe oryzae inducing defense resistance in rice

  Inducible defenses that contribute to overall resistance in plant can be triggered by elicitors. A novel elicitor, derived from the mycelia of the blast fungus Magnaporthe oryzae, was purified to homogeneity by HiPrep 16/20 DEAE-Sepharose FF, Concanavalin A-Sepharose 4B and HiPrep 16/60 Sephacryl S-100 column chromatography. The purified elicitor appeared as single band corresponding to a molecular...

Author(s):   Chunyan Ji and Zhenzhong Wang    

April 2012

Increasing the amylose content of maize through silencing of sbe2a genes

  Improved amylose content in maize has been achieved by reducing the starchbranching enzyme (SBE) activity via transgenic maize inbred line transformed by a high-efficient RNAi expression vector, which may provide the foundation for maize quality improvement. The sense and anti-sense fragments of maize SBE gene sbe2awere cloned by reverse transcript PCR and high efficient RNAi...

Author(s):   Shuyan Guan, Yiyong Ma, Huijing Liu, Siyan Liu, Guangna Liu, Lina Zhaoand Piwu Wang  

April 2012

Common vetch-wheat intercropping: Haylage yield and quality depending on sowing rates

  The winter-sowing genotypes of common vetch (Vicia sativa L.) are very susceptible to lodging and therefore are sown in mixtures with small grains that serve as supporting crops. In order to determine an optimum ratio of vetch and wheat (Triticum aestivum L.) components in their mixture, a four-year trial (autumn 2005 to spring 2009) was carried out, aiming at the yield and quality of winter vetch...

Author(s):   Karagic Dura, Mikic Aleksandar, Milosevic Branko, Vasiljevic Sanja and Dusanic Nenad  

April 2012

Physiology of seed yield in soybean: Growth and dry matter production

  A field study was conducted to assess the growth parameters controlling the dry matter and seed yield of soybean. The result shows that growth rate was slow during vegetative phase in all genotypes. A relatively smaller portion of total dry mass (TDM) was produced before flower initiation and the bulk of it after anthesis. Maximum absolute growth rate (AGR) was observed during pod filling stage...

Author(s):   M. A. Malek, , M. M. A. Mondal, M. R. Ismail, M. Y. Rafii and Z. Berahim  

April 2012

Effect of heavy metal and EDTA application on heavy metal uptake and gene expression in different Brassica species

  The present study investigates the effect of different concentration of heavy metals (Cd, Cr and Pb) and ethylenediaminetetraacetic acid (EDTA) application on two Brassica species (Brassica carinata and Brassica juncea). EDTA application had significant (p<0.05) effect on shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight and accumulation of heavy...

Author(s):   Madiha Iqbal, Jehan Bakht, Mohammad Shafi and Rafi Ullah  

April 2012

Genetic diversity in Chinese natural zoysiagrass based on inter-simple sequence repeat (ISSR) analysis

  Zoysiagrass (Zoysia sp.) is extensively used in turf establishment and livestock herbage due to its many outstanding characters. Native Zoysia sp. are widely distributed in China. Inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity and genetic relationships of 81 Chinese wild zoysiagrass accessions and three commercial cultivars. The...

Author(s):   Y. Xie, L. Liu, J. Fu and H. Li  

April 2012

An investigation on mechanisms of blanked nut formation of hazelnut (Corylus heterophylla fisch)

  The occurrence of blank nuts is common in Corylus heterophylla Fisch orchards of China. This study was aimed to find the possible mechanisms involved in blank nuts formation in wild C. heterophylla Fisch species. The effects of pollination, defoliation and girdling on fruit production of C. heterophylla Fisch were studied from northern China. The effect of pollination on various...

Author(s):   Jian-feng Liu, Yun-qing Cheng, Kun Yan and Qiang Liu  

April 2012

Effects of soil flooding on photosynthesis and growth of Zea mays L. seedlings under different light intensities

  Soil flooding is one of the major abiotic stresses that repress maize (Zea mays L.) growth and yield, and other environmental factors often influence soil flooding stress.This paper reports an experimental test of the hypothesis that light intensity can influence the responses of maize seedlings to soil flooding. In this experiment, maize seedlings were subjected to soil flooding...

Author(s):   Xiuping Wang, Tianxue Liu, Chaohai Li, and Hao Chen,  

April 2012

Propagation physiology of Juniperus phoenicea L. from Jordan using seeds and in vitro culture techniques: Baseline information for a conservation perspective

  Seeds of Juniperus phoenicea L. collected from Shouback city, south of Jordan, were cold-moist stratified at 5°C for 1 and 3 months. Non-stratified seeds were used as thecontrol. The recalcitrant nature of J. phoenicea was clear as shown by low germinability of seeds. The highest germination percentage was recorded for seeds stratified for three months. Germination bioassay of...

Author(s):   Ezz AL-Dein Al-Ramamneh, Susan Dura and Nidal Daradkeh    

April 2012

Lactic acid fermentation from refectory waste: Factorial design analysis

  A factorial experimental design method was used to optimize the lactic acid production using Lactobacillus bulgaricus from refectory waste obtained from Istanbul Technical University mess hall, Turkey. Fermentation experiments were carried out in a batch type reactor system which contains refectory waste with Lactobacillus bulgaricus bacteria during an exposition time of 30 h. Factorial...

Author(s):   Didem OMAY and Yuksel GUVENILIR  

April 2012

Optimization of growth parameters for increased yield of the edible mushroom Calocybe indica

  This study was conducted to enhance the yield performance of Calocybe indicathrough optimization of the cultivation parameters by utilizing cheaper substrates that are available in Tamil Nadu, India. The total fresh mushroom yield obtained with a change in spawn substrate, spawn running bed substrate (SRBS), sterilization procedure and casing soil with different amendments were studied. Sorghum...

Author(s):   Gopinath Lakshmipathy, Arunkumar Jayakumar, Meera abhilash and Shantha Prema Raj  

April 2012

Isolation and characterization of toebicin 218, a bacteriocin, produced by Geobacillus toebii HBB-218

  A novel bacteriocin Toebicin 218 was isolated from Geobacillus toebii HBB-218, a soil inhabiting Gram positive bacterium. The cell free culture supernatants of G. toebii HBB-218 showed antibacterial activity against many Gram positive bacteria including thermophilic strains. Purification of the bacteriocin was achieved after ammonium sulphate precipitation, gel filtration and ion exchange chromatography....

Author(s):   Gamze BaÅŸbülbül Özdemir and Haci Halil Biyik  

April 2012

Proximate and mineral analysis of some wild edible mushrooms

  Proximate and mineral analysis of five species of mushroom, Lentinus squarrosulus, Volvariella volvacea, Coprinus micaceus, Lepiota procera, and Auricularia auriculawere examined in this study to determine their nutritional value. These mushrooms were found to contain on the average, 3.24 to 8.70% of crude fibre; 4.12 to 11.12% of ash; 0.90 to 2.58% of fats and oil; 12.02 to 27.00% of...

Author(s):   I. O. Okoro and F. I. Achuba  

April 2012

Stabilization and preservation of probiotic properties of the traditional starter of African opaque sorghum beers

  This present study assessed the impact of drying process parameters, that is temperature and drying duration, on the dry matter content, pH, titratable acidity, yeasts and lactic acid bacteria content of granule starter of African opaque sorghum beer. Probiotic properties of the dry starter were tested. The aim was to establish levels of temperature and duration of drying that lead to a longer shelf life and...

Author(s):   A. P. Polycarpe Kayodé, Deloris C. Deh, Lamine Baba-Moussa, Simeon O. Kotchoni and Joseph D. Hounhouigan  

April 2012

Qianliening capsule treats benign prostatic hyperplasia (BPH) by down-regulating the expression of PCNA, CyclinD1 and CDK4

  Benign prostatic hyperplasia (BPH) is characterized by abnormal proliferation of epithelial and stromal cells in prostatic tissue, which is closely correlated with increased expression of PCNA, CyclinD1 and CDK4. Therefore, inhibition of cell proliferation by suppressing the expression of the above genes is a promising strategy in the development of novel anti-BPH therapies. The aim of this study was to...

Author(s):   Xiaoyong Zhong, Jiumao Lin, Jianheng Zhou, Wei Xu, Zhenfeng Hong and Jun Peng,  

April 2012

Isolation, purification and effects of hypoglycemic functional polysaccharides from Inonotus obliquus

  Inonotus obliquus is generally used for the treatment of diseases such as cancers, angiocardiopathy and diabetes. However, few studies are available on its functional components. The objective of this study was to isolate and purify hypoglycemic functional polysaccharides from I. obliquus (HPIO) and study their hypoglycemic activities. I. obliquus powder was used to obtain...

Author(s):   Tao Hu, Ping Liu, Yuanying Ni and Chuntao Lu  

April 2012

Morphological and chemical characteristics of tomato foliage as mechanisms of resistance to Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) larvae

  Morphological characters and chemical composition of tomato (Lycopersicon esculentum Miller) leaves were measured and compared among nine tomato varieties (Roma VFN, NARC-1, Fs-8802, Tommy, Pant Babr, Rio Grande, Nova Mecb, Pakit and Sahil) exhibiting varying levels of host plant resistance to Helicoverpa armigera(Hübner) (Lepidoptera: Noctuidae) as based on fruit infestation. The variety,...

Author(s):   Muahmmad Ashfaq, Muhammad Sajjad, Muhammad Noor ul Ane and Noureen Rana  

April 2012

Bacterial content in the intestine of frozen common carp Cyprinus carpio

  The quantitative and qualitative analyses of bacterial flora associated with the intestine of common carp Cyprinus carpio in fresh and frozen storage conditions for 16 months was determined. Aerobic plate counts (APC) ranged from 1.8 ± 2.7 × 109 to 1.1 ± 2.9 x 104 colony forming unit (CFU) g-1, 2.0 ± 2.1 x 1010 to 6.1 ± 1.4 x 104 CFU g-1,...

Author(s):   Ahmed H. Al-Harbi and Md. Naim Uddin  

April 2012

Generation and characterization of a stable red fluorescent transgenic Tanichthys albonubes line

  White cloud mountain minnow (Tanichthys albonubes) is a small cyprinid oviparous fresh-water fish in Southern China. For ornamental purpose, the red fluorescent protein (RFP) transgenic T. albonubes was generated by microinjection of a pMYLZ2-RFP gene construct containing zebrafish myosin light polypeptide 2 (mylz2) promoter into the fertilized eggs, and a transgenic line was...

Author(s):   Qing Jian, Min Chen, Jun-jie Bai, Peng Jiang, Jia-jia Fan, Xing Ye and Shi-lingXia  

April 2012

Soybean (Glycine max) as a versatile biocatalyst for organic synthesis

  A series of aliphatic and aromatic aldehydes and ketones were reduced using plant cell preparations of Glycine max seeds (soybean). The biotransformation of five aromatic aldehydes in water, at room temperature afforded the corresponding alcohols in excellent yields varying from 89 to 100%. Two prochiral aromatic ketones yielded the alcohol in very low conversion, 1% and to 4%; however with...

Author(s):   Luciana M. Bertini, Telma L. G. Lemos, Leonardo A. Alves, Francisco Jose Q. Monte, Marcos C. de Mattos and Maria da Conceição F. de Oliveira  

April 2012

Passive and active immunity against parvovirus infection in piglets

  The aim of this study was to come to a closer understanding of the origination, dynamics of movement and cessation of colostral immunity to parvovirus infection in swine (PPV) on the basis of an analysis of antibody titres in the blood serum of piglets in their first 6 months. On the third day of life in the blood serum of newborn piglets, an average antibody titer of 13.37 was recorded. An antibody...

Author(s):   Nenad Stojanac, Mladen Gagrčin, Ognjen Stevančević, Ivan Stančić and Aleksandar Potkonjak  

April 2012

The use of kefir as potential probiotic in Çoruh trout (Salmo coruhensis): Effects on growth performance and immunoglobulin (IgM) levels

  The objective of this study was to evaluate the effects of three different rates of kefir on growth performance and immunoglobulin (IgM) levels of Çoruh trout (Salmo coruhensis). The experiment was carried out with the four following treatments: Control group (not supplemented kefir), D1, D2 and D3 (kefir supplemented diet 10, 20, 40 g kg-1 fish body mass, respectively). Condition factor...

Author(s):   Erkan CAN, Filiz KUTLUYER, Fatma DELÄ°HASAN SONAY and Özay KÖSE  

April 2012

Molecular cloning and characterization of a novel Cys2/His2-type zinc finger protein gene from chrysanthemum

  A novel member of the Cys2/His2-type zinc finger protein gene family, designatedDgZFP3, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE). The DgZFP3 encodes a protein of 248 amino acids, including two conserved Cys2/His2-type zinc finger motifs with a plant-specific QALGGH motif in each zinc finger domain, a B-box (KXKRSKRXR) domain in the N-terminal region as a putative...

Author(s):   Qing-Lin Liu, Jiao Wu, Ke-Dong Xu, Liang-Jun Zhao and Hai-Qing Zhang