African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Table of Content: 12 March, 2014; 13(11)

March 2014

Recommended temperature and relative humidity for storage of Brazilian tropical flowers

Post-harvest treatment and proper handling is essential so that the flowers can maintain their quality during marketing and exporting. This review aims to gives information on the storage temperature used in postharvest handling of tropical flowers of Brazil. This review provides detailed specifications and recommendations for storing the six main Brazilian tropical flowers.   Key words: Floriculture,...

Author(s): Marcos Ribeiro da Silva Vieira, Adriano do Nascimento Simões and Pahlevi Augusto de Souza

March 2014

Molecular discrimination and genetic relationships between some cultivars of Cucurbita pepo ssp. pepo using random amplification of polymorphic DNA (RAPD) analysis

Cucurbita pepo ssp. pepo; zucchini group is a widely grown and economically important group belonging to genus Cucurbita, and being one of the easiest groups to cultivate in temperate climate with overwhelming production. Since, RAPD analysis provides a fast and reliable method for molecular characterization and investigation of the intraspecific genetic relationships; it had been used in this study to discriminate and...

Author(s): Safaa Abdel Aleem Radwan

March 2014

Genetic variation of twenty autosomal STR loci and evaluate the importance of these loci for forensic genetic purposes

The aim of this study was of twofold. One was to determine the genetic structure of Iraq population and the second objective of the study was to evaluate the importance of these loci for forensic genetic purposes. FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. Twenty (20) STR loci and Amelogenin), including D3S1358, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, THO1, vWA,...

Author(s): Imad Hadi, Mohammed Abdullah, Aamera Jaber and Cheah Yoke

March 2014

Effects of different media concentartions on callogensis in sugar cane (Saccharum officinarum L.)

Sugarcane (Saccharum officinarum L) var. 668 was propagated through tissue culture technique: callogenesis at MS media supplemented with different concentrations of growth regulators. Explants were disinfected with 70% ethanol which was sprayed directly on outer most sheathe which covered the tender stem to be used as source of explants. After 15 days, those explants which survived turned into green leaves and were used...

Author(s): Waleed Ahmad Khattak, Mazhar Ul-Islam and Ihsan Ullah,

March 2014

Effect of overstory on the seasonal variability of understory herbs in primary broad-leaved Korean pine forest of Changbai Mountain

In order to study the structure of herbaceous biodiversity and its effect factors in broad-leaved Korean pine forest, a total of 100 1-m2 sub-plots were established in the 1 ha site which was monthly investigated from May to September in 2009. Data on soil attributes were also investigated (soil organic matter, available nitrogen, available phosphorus, available potassium, soil, water and pH) without light environment...

Author(s): Xia Fuxai, Folega Fousseni, Pan Chungang, He Huaijiang and Zhao Xiuhai

March 2014

Air pollution tolerance indices of some plants around Ama industrial complex in Enugu State, Nigeria

Green house gases pollute the atmosphere and cause global warming which has become a worldwide environmental problem. In order to evaluate the ability of plants to absorb such gases, the air pollution tolerance indices (APTI) of some plants around an industrial complex in Nigeria, were determined. The total chlorophyll, ascorbic acid, pH, and relative water content of the leaf extracts of Mangifera indica, Delonix...

Author(s): Nwadinigwe, A. O.

March 2014

Effect of road side dust pollution on the growth and total chlorophyll contents in Vitis vinifera L. (grape)

The effect of dust in Vitis vinifera L. on its pigmentation and growth was studied in 2012. Measurements were taken for plants in the Campus University of Balochistan, Quetta. A significant reduction in plant length, cover, number of leaves and total chlorophyll contents for V. vinifera L. was observed. The maximum reduction of all investigated variables such as plant length, cover, number of leaves and total...

Author(s): Saadullah Khan Leghari, Mudassir Asrar Zaid, Atta Muhammed Sarangzai, Muhammed Faheem, Gulam Razaq Shawani and Waris Ali

March 2014

Improving oil biodegradability of aliphatic crude oil fraction by bacteria from oil polluted water

Water samples were collected from three oil polluted stations, two replicates for each station, from southern region of Shatt Al-Arab estuary, and southern of Basrah city during the period from September to October 2011. The mineral salts medium was used to isolating oil biodegrading bacteria. Four bacterial species were identified according to their morphological and biochemical profiles as: Aeromonas hydrophila,...

Author(s): Amin A. Alsulami, Asaad M. R. Altaee and Fadhil N. A. Al-Kanany

March 2014

Yield of maize (Manoma spp) affected by automobile oil waste and compost manure

The study is aimed at evaluating the effects of compost manure on the remediation of automobile oil waste polluted soils, and on the yield parameters (plant height, leave surface and dry matter weight) of maize (Manoma spp). Analyses of soil samples of contaminated and uncontaminated sites collected with core samplers at 10 cm depth and auger samplers at two depths (surface, 0 – 20 cm; subsurface, 20 – 40...

Author(s): Ezeaku, P. I, and Egbemba, B. O

March 2014

Biodegradation of hydrocarbon compounds in Agbabu natural bitumen

The recovery of an environment polluted by petroleum and allied hydrocarbons through bioremediation is being embraced globally as the best technology of removing hydrocarbon pollutants from environment. Infrared spectral changes and gravimetric analysis from the preliminary biodegradability study carried out on Agbabu Natural Bitumen showed the vulnerability of the bitumen to some bacteria: Pseudomonas putrefaciens,...

Author(s): Ojeyemi M. Olabemiwo, Adediran, G. O., Adekola, F. A., Adelowo, O. O. and Olajire, A. A.

March 2014

In vivo study of lens regeneration in Rana cyanophlyctis under influence of vitamin A and ascorbic acid

After removal of lens from eye of Rana cyanophlyctis, the pigmented epithelial cells of dorsal iris started to proliferate and dedifferentiate and consequently regenerate lens. Vitamin A and ascorbic acid enhanced the percentage lens regeneration not only in young tadpoles but also in froglets. Lens regeneration ability declined with age of animals in both control as well as treated groups.   Key words:...

Author(s): Sumer Singh and O. P. Jangir

March 2014

Identification and characterization of milk-clotting proteases produced by two species of mold

Two strains of fungi were isolated and identified as Aspergillus tamarii and Penicillium pinophilum which showed good enzymatic activity on casein, 1933.33U and 1822, 21U, respectively. The search for milk clotting enzymes by fermentation at acid pH on culture medium containing whey and, after purification by molecular exclusion chromatography, affinity chromatography and SDS-PAGE helped to find two enzymes having a...

Author(s): Aïcha Benlounissi,  Aïcha Mechakra-Maza, Loïc J. Blum and Christophe A. Marquette#

March 2014

Biochemical and cytological analysis of five cultivars of Cicer (chickpea)

During the present study, protein estimation and protein profiling of five cultivars of Cicer namely Dhawal, ujjwal, Shubhra, DCP-92-3 and IPC-04-20 have been carried out. The protein content on per gram fresh weight basis was found highest in the Dhawal measuring 35.2± 3.83 mg followed by DCP-92- 3 and lowest was found in the IPC-04-20 cultivar. The protein profiling of seed proteins using sodium dodecyl...

Author(s): Jahangir, A. D., Shahzada Arshid, Kudesia, R., Srivastava, M. K. and Aijaz A. Wani,

March 2014

Screening of Candida boidinii from Chemlal spent olive characterized by higher alkaline-cold adapted lipase production

A total of 24 lipolytic yeasts were isolated from the spent olive derived from olive fruits of the Algerian variety Chemlal. One strain, G5, had the highest lipolytic activity (20 mm) on the tributyrin agar plate. The morphological, biochemical characterization and 18S rDNA gene analysis of the selected strain, confirms that it is Candida boidinii KF156789. The production of lipase and biomass were carried out in liquid...

Author(s): Insaf Bataiche, Noreddine Kacem-chaouche, Jacqueline Destain, Annick Lejeune and Philippe Thonart

March 2014

Jabuticaba [Pliniajaboticaba (Vell.) Berg] skins decrease lipid peroxidation: Hepatoprotective and antihyperlipidemic effects

The effect of Jabuticaba [Plinia jaboticaba (Vell.) Berg] Skin Flour (JSF) was studied on peroxidation, plasma and hepatic lipid profiles of female rats, as well as quantification and characterization of phenolic compounds. The animals were divided into four groups of eight rats. The groups received 0 (control); 0.5; 1.5 and 3.0 g JSF per 100 g diet. The diet with 3.0% JSF increased the HDL level by 20.23% compared to...

Author(s): LAGE Fabíola Fonseca, SIMÃO, Anderson Assaid, GUEDES Mayara Neves Santos, RAMOS Vinicius de Oliveira, SOUSA Raimundo Vicente de and CORRÊA Angelita Duarte

March 2014

Antitrichomonal activity of Acanthospermum hispidum D. C. (Asteraceae)

Acanthospermum hispidum (Asteraceae), used ethnomedically in the treatment of inflammatory conditions and fever, was evaluated for antiprotozoal activities such as trypanocidal and antiplasmodial effects. This study was carried out to investigate the anti-trichomonal potential of the plant. The air-dried leaf was extracted successively with petroleum ether, chloroform, ethylacetate and methanol using the soxhlet...

Author(s): A. O. Adepiti, C. O. Adewunmi and J. M. Agbedahunsi

March 2014

In vitro microrhizome production in Decalepis hamiltonii

Microrhizomes were produced from leaf disc derived callus of a threatened endemic medicinal plant - Decalepis hamiltonii. Murashige and Skoog (MS) medium supplemented with 2 mM 6-benzyleaminopurine (BAP) and 6 mM 1-napthaleneacetic (NAA) acid was found to be optimum for rapid callus induction and establishment from leaf disc explants. Further differentiation of callus into microrhizome was conquered in MS medium...

Author(s): K. Thangavel, P. Ravichandran,   M. G. Ebbie and V. Manimekalai