African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12081

Table of Content: 14 February, 2018; 17(7)

February 2018

Isolation, purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus thuringinsis-SH-II-1A

In the present study, the isolation, purification and partial characterization of thermostable serine alkaline protease produced from Bacillus thuringinsis SH-II-1A was reported. The culture was isolated from soil of slaughter house waste and identified further from ribosomal sequence. The crude enzyme was purified by ammonium sulfate precipitation, dialysis and Sephadex G-200 gel permeation chromatography up to 17.04...

Author(s): Sunil L. Harer, Manish S. Bhatia and Neela M. Bhatia

February 2018

Cucurbit yellow stunting disorder virus and watermelon chlorotic stunt virus induced gene silencing in tobacco plants

Cucurbit yellow stunting disorder virus (CYSDV) and Watermelon chlorotic stunt virus (WmCSV) are the most widespread and damaging viruses to cucurbits in the Middle East. CYSDV and WmCSV are cucurbit-infecting bipartite whitefly-transmitted viruses. Post-transcriptional gene silencing (PTGS) is a universal mechanism by which plants are able to systemically switch off the expression of targeted genes via the reduction of...

Author(s): Ahmed Mohamed Soliman, Adel Abdelsabour Rezk, and Khalid Abdallah Alhudaib

February 2018

Tomato yellow leaf curl virus: Diagnosis and metabolites

The existence of Tomato yellow leaf curl virus (TYLCV) was figured out in different locations in Al-Ahsaa of Saudi Arabia. Polymerase chain reaction (PCR) results of samples collected showed that TYLCV existed in all locations. Using AVcore and ACcore primers, begomoviruses family were detected in symptomatic tomato plants and by using TYv2664 and TYc138 (specific primers for the detection of TYLCV), the results proved...

Author(s): Ahmed Mohamed Soliman, and Maged Elsayed Mohamed,

February 2018

Polymerase chain reaction amplification of 16S rDNA from three nosocomial bacterial isolates in Kaduna State, Northern Nigeria

A wide variety of opportunistic pathogens has been detected in hospital surfaces. Medical center surfaces can serve as reservoirs of pathogenic bacteria. Among this pathogens, Pseudomonas species are one of the leading causes of nosocomial infections, frequently found in hospital environments. Polymerase chain amplification system remains one of the best methods for the rapid detection of low numbers of pathogenic...

Author(s): Richard, R., Denwe, S. D.., Yerima, M. B. and Olatunde, S. K.

February 2018

The nitrogen-fixing Frankia significantly increases growth, uprooting resistance and root tensile strength of Alnus formosana

Restoration of Alnus formosana (Burk.) Makino on landslide areas is important for agroforestry, forestry and soil erosion control in Taiwan. To ensure successful reforestation, A. formosana seedlings have to develop strong root system for nutrient and water acquisition as well as anchorage. Inoculating of A. formosana with symbiotic nitrogen-fixing actinobacteria Frankia may help mitigate drought and nutrient...

Author(s): Jung-Tai Lee and Sung-Ming Tsai

February 2018

Characterization of biodiesel obtained from atemoya (Annona squamosa × A. cherimola) seed oil

Biodiesel is derived from renewable sources, such as vegetable oils, by means of a transesterification process in which triacylglycerols are transformed into smaller molecules of esters of fatty acids and glycerol. The transesterification reactions of ‘Gefner’ atemoya (Annona squamosa × A. cherimola) seed oil extracted by pressing (physical) and solvent (chemical) processes were studied, with analysis...

Author(s): Luciana Soares Da Cruz, Bárbara Lemes Outeiro Araújo, Luiz Roberto Marques Albuquerque, Pedro Castro Neto, Angelita Duarte Corrêa and Luciana de Matos  Alves Pinto