August 2018
In vitro regeneration of Neocarya macrophylla (Sabine) Prance, wild fruit of Niger
Neocarya macrophylla is a spontaneous fruit species that constitutes the dominant population of Dallol Bosso in Southwestern Niger. In order to test the in vitro regeneration ability of this species, micro-propagation was applied to micro-cuttings from young almond seedlings. The Murashige and Skoog basal medium was enriched with several concentrations of naphthaleneacetic...
August 2018
Biomanagement of Rose and carnation wastes in flower industries with three epigeic earthworm species: Eisenia fetida, Eisenia andrie and Dendrobanae veneta
The horticulture sector in Ethiopia is being challenged by multifaceted hitches, among which managing excess wastes produced from the farms and minimizing the cost of inorganic fertilizers are the major ones. The performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing flower (Rose and Carnation) wastes through vermicomposting was evaluated. The study was done...
August 2018
Effect of sample extraction, preparation methods on HPLC quantification of plumbagin in in vivo and in vitro plant parts of Plumbago zeylanica L.
Plumbagin is an important therapeutic compound of Plumbago zeylanica L., the commercial demand of this compound warrants optimizing a suitable method to isolate plumbagin in large scale. The present study was undertaken to obtain the maximum recovery of plumbagin content by employing different extraction methods viz., ultra-assisted extraction (UAE), maceration extraction (ME), soxhlet extraction (SE), serial soxhlet...
August 2018
Co-expressing house fly cytochrome P450 6A1 and human cytochrome P450 reductase in Escherichia coli to bioconvert insecticide aldrin
Aldrin used in agricultural as well as industrial or home pest control is known to be a persistent organic pollutant, having chronic adverse effects on wildlife and humans. The degradation of aldrin has drawn a lot of attentions. In this study, house fly cytochrome P450 6A1 (CYP 6A1) or both house fly cytochrome P450 6A1 and human NADPH-P450 reductase (CPR) together was expressed in Escherichia coli cells to study their...
August 2018
Isolation and identification of bacterial endophytes from Crinum macowanii Baker
The widespread distribution of Crinum macowanii across the African continent has entrenched the plant’s medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and...
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