August 2012
Cloning and semi-quantitative expression of endochitinase (ech42) gene from Trichoderma spp.
Species of Trichoderma such as T. harzianum, T. viride and T. atroviride are some of the most potent antagonistic fungi, and have been used as plant growth promoters in developed countries. Endochitinase (ech42) gene which is involved in mycoparasitism, was isolated from Trichoderma spp. taken from hot-arid soils of Rajasthan, cloned, sequenced and its expression...
August 2012
Effects of electron beam radiation on trait mutation in azuki bean (Vigna angularisi)
Dry seeds of azuki bean (Vigna angularisi), Jingnong 6 and Hebei 801 varieties were irradiated by electron beam of 100, 300, 600, 700 and 900 Gy, respectively. Mutations of leaf shape and color, seed size and shape, trailing, more branching, dwarfing, early or late flowering time and high yield were created in...
August 2012
Identiï¬cation of differentially expressed sequences in bud differentiation of oriental hybrid lily cultivar ‘Sorbonne’ via suppression subtractive hybridization
The developmental process of lily flower bud differentiation has been studied in morphology thoroughly, but the mechanism in molecular biology is still ambiguous and few studies on genetic expression have been carried out. Little is known about the physiological responses of flower bud differentiation in Oriental hybrid lily ‘Sorbonne’ (Lilium spp.) during the stages of...
August 2012
Molecular characterization of genetic diversity in some durum wheat (Triticum durum Desf.) in Palestine
Random amplified polymorphic DNA (RAPD) analysis was conducted to estimate the genetic diversity and relatedness among 10 wheat cultivars and landraces. Five out of 70 decamer random primers showed distinctly polymorphic bands when used to amplify genomic DNA. The primers yielded a total of 157 RAPD markers of which 150 were considered as polymorphic. Each primer produced 18 to 37 polymorphic...
August 2012
Cost-effective nutrient sources for tissue culture of cassava (Manihot esculenta Crantz)
Application of tissue culture technology is constrained by high costs making seedlings unaffordable. The objective of this study was to evaluate the possibility of using locally available fertilizers as alternative nutrient sources for cassava micropropagation. A Low Cost Medium (LCM) whereby the conventional sources of four Murashige and Skoog (MS) macronutrients had been replaced with locally available...
August 2012
Large scale production of antitumor cucurbitacins from Ecballium Elaterium using bioreactor
Bioreactor plays a vital role in the commercial production of secondary metabolites and pharmaceuticals from plant cells. Many physical factors, like the mixing intensity, shear stress and operation conditions were optimized as a first step in scaling up process. Qualitative and quantitative determination of cucurbitacins E and I in the extract were carried out using high-performance liquid chromatography...
August 2012
Evaluation of the expression of internal control transcripts by real-time RT-PCR analysis during tomato flower abscission
Many investigations of the abscission mechanisms in plants are based on gene expression studies. Quantitative real-time RT-PCR (qRT-PCR) is widely and increasingly used for mRNA quantification, and the results are typically referenced to an internal control gene to avoid bias. We investigated the suitability of twelve tomato(Lycopersicon esculentum Mill. var. Liaoyuanduoli) housekeeping genes during...
August 2012
Expression analysis of banana MaECHI1 during fruit ripening with different treatments
The main function of endochitinase is believed to be pathogenesis related protein. However, more and more scientists reported the roles of endochitinase in plant growth and development. In order to investigate the role of endochitinase in postharvest banana fruit ripening, an endochitinase gene known as MaECHI1 had been isolated from a suppression subtractive hybridization (SSH) complementary...
August 2012
Effect of 1-methylcyclopropene and vacuum cooling treatments on post harvest quality of Salicornia bigelovii Torr.
Salicornia bigelovii Torr. is a potential human food supplement that can be cultivated in subtropical salt marsh with seawater irrigation. However, this plant is not only perishable but also highly seasonal. The objectives of this study were to examine if the prestorage treatments with several concentrations of 1-methylcyclopropene (1-MCP) from 0 to 2.5 mL L-1, and vacuum cooling would...
August 2012
Accelerated ripening of Kashar cheese with encapsulated protease
In this study, protease enzymes were encapsulated in k-carragenan, gellan andsodium alginate using emulsion and extrusion techniques and were then added in cheese milk together with rennet. The effects of the encapsulating material and ripening period on the chemical, textural and sensory characteristics of Kashar cheese were investigated. The study demonstrated that sodium alginate, gellan...
August 2012
Effects of electromagnetic field of 33 and 275 kV influences on physiological, biochemical and antioxidant system changes of leaf mustard (Brassica chinensis)
The effects of electromagnetic fields (EMF) from 33 and 275 kV high voltage transmission line on biochemical and antioxidant system changes in mustard leaf (Brassica chinensis) were investigated under field condition. Mustard leaves were exposed to EMF from power lines at distances of 0, 3, 6, 9, 10, 12, 15, 18, 20, 21, 30, 40, 50 and 60 m away from the 33 kV power line and at 0, 10, 20, 30, 40,...
August 2012
Overwintering physiology of the rice stem borer larvae, Chilo suppressalis Walker (Lepidoptera: Pyralidae): Roles of glycerol, amino acids, low-molecular weight carbohydrates and antioxidant enzymes
The rice stem borer, Chilo suppressalis (Walker), is a major rice pest around the world. A strong ability of the rice stem borer to adapt/resist cold temperature (cold hardiness) contributes to its survival through winter. However, the physiological mechanism of its cold hardiness is poorly understood. In this study, we determined the supercooling points (SCPs), the contents of amino acids and...
August 2012
Transcriptional peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1α) regulates transformation of muscle fiber type in Schizothorax prenanti
Peroxisome proliferator-activated receptor γ coactivator (PGC)-1É‘, a well-known member of PGC-1 transcriptional coactivator’s family, plays a key role in various metabolic pathways. Here, we investigated the role of PGC-1É‘ in the transformation of muscle fiber type in Schizothorax prenanti. The expression of PGC-1É‘ was induced inS. prenanti muscles following fasting....
August 2012
Effect of acrylic acid on the properties of polyvinylpyrrolidone hydrogel prepared by the application of gamma radiation
Hydrogels based on polyvinylpyrrolidone (PVP) networks grafted with acrylic acid (AAc) was prepared by using γ-rays from a Co-60 source at room temperature. The parameters like effect of radiation dose and concentration of AAc were studied. The properties such as gel content, swelling behavior and thermal stability were also evaluated. The result indicated that gel content of hydrogel...
August 2012
Preparation and characterization of water-absorbing composite membrane for medical applications
The present work introduces a novel method for the formation of water absorbing composite membrane. The prepared composite is based on chitosan, carboxymethyl cellulose (CMC), and Montmorillonite. Prepared composite membrane exhibits high water absorbing and holding capacity with increasing clay content. The water absorbency was studied as a function of the hydrogel composition and the medium pH....
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