August 2010
Biotechnological uses of Azotobacter vinelandii: Current state, limits and prospects
Azotobacter vinelandii is a bacterium obligate aerobe able to fix nitrogen and grow under microaerophilic conditions. This microorganism has remarkable cellular machinery with the ability to biosynthesize three molecules of important biotechnological and biomedical applications: the extracellular polysaccharide alginate, the intracellular polyester poly-b-hydroxybutyrate (PHB) and siderophores compounds. Alginate...
August 2010
Strategic considerations for Chinese agricultural water and food safety: Issues, challenges and suggestions
China is a country not only with water resources shortage, but also with a large population and great food needs. Food security issues have aroused great concern at home and abroad. It has become an inevitable choice for sustainable development of Chinese economic society to achieve food and strategic water security. Based on related analysis of current data as agricultural water consumption, effective irrigation, food...
August 2010
Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene
The tumor-suppressor gene p53 and its downstream genes consist of a complicated gene network, and the challenge to understand the network is to identify p53 downstream genes. In order to isolate and identify new p53 regulated genes, we constructed and characterized a normalized cDNA library from human brain glioma cell line U251 while exogenous p53 gene is overexpressed. The constructed cDNA library contained...
August 2010
Molecular characterization, polymorphism of growth differentiation factor 5 gene and association with ultrasound measurement traits in native Chinese cattle breeds
Growth differentiation factor 5 (GDF5), involved in the development and maintenance of bone and cartilage, is an important candidate gene for growth and carcass traits selection through marker-assisted selection (MAS). Genomic structural analysis showed that bovine GDF5 shares much similarity with humanGDF5. The latest findings demonstrate that the single nucleotide polymorphism (SNP) T586C in exon 1 is...
August 2010
Regression of mouse-derived renal cancer by adoptive transfer of tumor-reactive RNAi-induced TGF-beta-insensitive CD8+ T cells
Transforming growth factor beta (TGF-beta) is a potent immunosuppressant. The present study was conducted to develop a treatment strategy through adoptive transfer of tumor-reactive RNAi-induced TGF-beta-insensitive CD8+ T cells. BALB/c mice were primed with irradiated Renca cells. CD8+ T cells were isolated from the spleen of primed animals, expanded ex vivo and were rendered TGF-beta-insensitive by...
August 2010
Gene cloning of phenolic acid decarboxylase from Bacillus subtilis and expression in top-fermenting yeast strain
Phenolic acid decarboxylase (PADC) gene, encoding phenolic acid decarboxylase, was cloned from Bacillus subtilis and ligated with a shuttle vector YEp352 to generate a novel plasmid YPADC. By analysis of sequencing and the restriction endonuclease digestion, the validity of construction was proved. Subsequently, the new vector was successfully transformed into wild-type top-fermenting yeast strain W303-1A; the...
August 2010
Recombinant expression and purification of L2 domain of human epidermal growth factor receptor
Epidermal growth factor receptor (EGFR) is one of the key molecules in cell growth and multiplication and plays an important role in some malignant processes. L2 domain of extra-cellular part of this receptor involved in ligand binding and its inhibition can prevent activation of related signaling pathways. The aim of the present study was cloning and expressing the fragment coding for L2 region of human EGFR for the...
August 2010
Cloning and expression pattern of chitin synthase (CHS) gene in epidermis of Ectropis obliqua Prout
Ectropis obliqua Prout is a major pest in tea fields. Chitin synthase (CHS) plays an important role in biosynthesis of chitin and growth of the pest. A cDNA sequence encoding the CHS and its expression pattern during development of E.obliqua was investigated. The CHS cDNA sequence was 5496 bp nucleotides, with an open reading frame of 4692 bp encoding a protein of 1563 amino acids. It belonged to CHS-A...
August 2010
Performance of Cochorus olitorius as influenced by soil type and organic manure amendments in Yewa North Local Government Area, Ogun State
An experiment was conducted in Yewa North Local Government Area, Ogun State to evaluate the effect of four soil types (loamy, sand, clay and silt) amended with poultry manure (PM), plant manure (PLM), sheep/goat manure (SGM) and NPK on performance of Cochorus olitorius. It was a factorial experiment in completely randomized design. Pre-treated seeds were planted and watered daily. Agronomic data collected on days...
August 2010
Diversity of arbuscular mycorrhizal fungi in Camellia sinensis in Uttarakhand State, India
Many microorganisms form symbioses with plants that range on a continuous scale, from parasitic to mutualistic. Among these, the most widespread mutualistic symbionts is the arbuscular mycorrhiza, formed between arbuscular mycorrhizal (AM) fungi and vascular flowering plants and other plants. A study of diversity of arbuscular mycorrhizal fungi in Camellia sinensis was conducted in four plantation territories...
August 2010
Influence of soil drought stress on photosynthesis, carbohydrates and the nitrogen and phophorus absorb in different section of leaves and stem of Fugi/M.9EML, a young apple seedling
Drought is the most significant limiting factor for plant cultivation. Greenhouse-grown 1-year-old potted M.9EMLA apple trees (Malus domestica Borkh.) (after growing for 6 weeks) were subjected to drought stress by withholding water for an additional six-week period. The photosynthesis, carbohydrates and the nitrogen (N), phosphorus (P) absorb in the upper, middle and lower leaves of the seedlings were determined....
August 2010
Nitrogen determination on tomato (Lycopersicon esculentum Mill.) seedlings by color image analysis (RGB)
In order to investigate the effectiveness of a new method based on color image analysis and the Minolta SPAD-502 chlorophyll meter for the diagnosis of nitrogen deficiencies of tomato seedlings, a field experiment was conducted. In this study, five levels of nitrogen fertilization were established so as to induce nitrogen deficiencies in tomato seedlings. Thirty-five days after sowing, total nitrogen was evaluated by...
August 2010
Changes in specific activity of ascorbate peroxidase during seed development of pea (Pisum sativum L.) treated with salicylic acid
A field split-split plot experiment in randomized complete block design was conducted during years 2003-04 and 2004-05 to evaluate the effects of salicylic acid (SA) at concentrations of 0, 10-4 and 10-5 M on four pea varieties (Meteor, Climax, Greenfeast and Rondo). Four phenological growth stages were selected of seed and fruit development (BBCH 73, BBCH 77, BBCH 83 and BBCH 88) for enzyme assay. Aqueous SA...
August 2010
Differential expression of ozone-induced gene during exposures to salt stress in Polygonum sibiricum Laxm leaves, stem and underground stem
The response of plants to environmental stresses is characterized by a number of physiological and biochemical changes that ultimately result from the selective increase or decrease in the biosynthesis of a large number of distinct proteins. In this report, we describe the characterization of an ozone-induced transcript, PcOZI-1, which has been identified from Polygonum sibiricum Laxm by the rapid...
August 2010
Effects of potassium and humic acid on emergence, growth and nutrient contents of okra (Abelmoschus esculentus L.) seedling under saline soil conditions
The effects of potassium and humic acid (HA) on emergence, growth and nutrient contents of okra (Abelmoschus esculentus L cv. Sultani) seedlings in saline soil conditions were evaluated. Different levels of humic acid (0, 500, 1000 and 1500 mg kg-1) and potassium (0, 75, 150 and 300 mg kg-1) were applied on growing media treated with 50 mg NaCl kg-1 before seed sowing. The experiment was designed as randomized...
August 2010
Comparative evaluation of different embryo rescue techniques on parthenogenetic melon (Cucumis melo L.) fruits induced with irradiated pollen
The methods of dihaploidization ensures important advantages in obtaining pure lines by rapid fixation of homozygosity. "Irradiated pollen technique" was used in this study. Disease resistant F1 hybrid variety candidates were utilized as the plant material. For the parthenogenetic induction, 300 Gray gamma irradiation were applied to the pollens and pollinations were conducted on the day after...
August 2010
A potential explanation for the effect of carbon source on the characteristics of acetate-fed and glucose-fed aerobic granules
This paper proposes a new theory to account for the effect of carbon source on the characteristics of acetate-fed and glucose-fed aerobic granules. It is well known that reactor pH can vary in response to the oxidation of glucose or sodium acetate. As such, the effects associated with the carbon sources may be explained by the changed pH. The proposal was explored by experiments. Aerobic granules were cultivated in...
August 2010
Effects of salinity stress on seedlings growth, mineral nutrients and total chlorophyll of some tomato (Lycopersicum esculentum L.) cultivars
In this study, six cultivars of tomato (Lycopersicum esculentum L. var. Jaguar, Xewel, Nadira, Lindo, Mongal and Ninja) were evaluated. They were subjected to salt stress during vegetative growth. Three concentrations of salt solution 50, 100 and 200 mM NaCl and the control (Wacquant nutrient solution) were used in irrigation. The total chlorophyll, the dry weight of seedlings (roots dry weight, stems dry weight...
August 2010
Environmental effects on the stable carbon and oxygen isotopic compositions and skeletal density banding pattern of Porites coral from Khang Khao Island, Thailand
The timing of band formation and linear skeletal growth rate based on environmental changes were investigated using alizarin red S (ARS) in Porites lutea coral at Khang Khao Island, the Gulf of Thailand from November 10, 1999 to March 15, 2001. The X-radiograph of the vertical section of the Porites coral skeleton was examined and three pairs of density bandings including intermediate bandings were...
August 2010
High folate production by naturally occurring Lactobacillus sp. with probiotics potential isolated from dairy products in Ilam and Lorestan provinces of Iran
Rapidly proliferating cells require large amounts of folate to support efficient DNA replication, repair and methylation indicating the importance of folate in human metabolism. Milk products are good sources of such vitamins which are produced by probiotics. In order to find suitable strains capable of high folate production, isolation and identification of Lactobacilli in traditional fermented milk from two...
August 2010
Effects of agitation speed, temperature, carbon and nitrogen sources on the growth of recombinant Lactococcus lactis NZ9000 carrying domain 1 of aerolysin gene
Lactococcus lactis is a Gram-positive bacterium widely used in the production ofbuttermilk and cheese. Recently, the bacterium becomes famous as the genetically modified organism can be used alive for the treatment of disease. In this study,different cultural conditions based on agitation speed and temperature on the growth of recombinant L. lactis NZ9000 harboring domain 1 of...
August 2010
Combined amino acids modulation with H2O2 stress for glutathione overproduction in Candida utilis
Strategies of amino acids addition coupled with H2O2 stresses were developed for glutathione (GSH) overproduction in high cell density (HCD) cultivation of Candida utilis. Based on the fact that glycine shows two functions of promoting cells growth as well as GSH production, precursor amino acids modulations of feeding glycine at 4 mmol/l/h at exponential phase and adding precursor amino...
August 2010
Antioxidant and antibacterial activities of Piliostigma reticulatum (DC.) Hochst extracts
The methanol and aqueous extracts of Piliostigma reticulatum barks were investigated for its antioxidant and antibacterial activities. The antioxidant test using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method demonstrated important radical scavenging activity for the methanol extract with IC50 = 0,37 ± 0,04 µgmL-1. The 2 extracts were then examined for antibacterial activity by using...
August 2010
Virus efficacy of recombined Autographa californica M nucleopolyhedrovirus (AcMNPV) on tea pest Ectropis obliqua
Ectropis obliqua is a major tea pest and chitin synthase (CHS) plays a key role in the pest growth and development. A 192 bp conserved domain from E. obliqua CHS gene was cloned and it was used to construct recombined Autographa californicaM nucleopolyhedrovirus (AcMNPV) with double-stranded RNA interference (dsRNAi) method. The recombined AcMNPV virus could propagate in host cells sf9. Injection...
August 2010
Effect of 10% sodium ascorbate on Streptococcus mutans adherence to bleached bovine enamel surface
Sodium ascorbate has been suggested to modify bleaching agents’ side effects especially on composite resin bonding to dental hard tissues. The aim of the present study was to evaluate the effect of 10% sodium ascorbate onStreptococcus mutans adherence to bleached enamel surfaces. Sixty enamel slabs from bovine incisors were used. After sterilization of the intact enamel surfaces with UV light, the specimens...
August 2010
Optimization of divalent cation in Saccharomyces pastorianus medium conditions for ethanol production
Cassava starch fermentations were conducted in batch cultures to optimize the effect of divalent cations on ethanol production with Saccharomyces pastorianususing the central composite rotatable response surface design. Divalent cations used were magnesium (Mg2+), zinc (Zn2+) and calcium (Ca2+). Maximum ethanol concentration of 11.12% v/v was obtained with cationic concentration combination of 64, 0.48 and 30 mg/l...
August 2010
Allelopathy regulates wheat genotypes performance at the enhancement stage by soil water and prohydrojasmon (PDJ)
Growth adaptation and allelopathic potential of four winter wheat (Triticum aestivumL.) accessions has been investigated in pot experiments by prohydrojasmon (PDJ, 10 - 5M) and soil water (75 and 45%) at the enhancement stage. This paper also presented the performance of photosynthesis, water use and weed suppression. The effect of soil water and PDJ on...
August 2010
Antioxidant enzymes as bio-markers for copper tolerance in safflower (Carthamus tinctorius L.)
Activities of antioxidants enzymes were investigated in order to evaluate protective mechanism of plants against oxidative stress induced by high concentration of copper. Safflower (Carthamus tinctorius L.) plants were exposed to 100 µM copper (Cu) for two weeks under controlled environmental conditions using hydroponic culture. Cu induced changes in chlorophyll and carotenoids pigments. More Cu accumulated...
August 2010
Transfusion transmitted malaria in three major blood banks of Peshawar, Pakistan
Malaria is one of the most important transfusion associated infections in many parts of the world, particularly the developing countries where it is endemic. This study estimates the risk of acquiring malaria from a single unit of blood in North of Pakistan. A prospective study was conducted to investigate transfusion transmitted malaria in three major blood banks of Peshawar, Pakistan. A total of 1558 (1534 males and...
August 2010
Development of a new medium containing date syrup for production of bleomycin by Streptomyces mobaraensis ATCC 15003 using response surface methodology
A combined statistical approach of orthogonal design and polynomial regression were applied to optimize the composition and concentration of a liquid fermentation medium for the production of bleomycin (BLM) by Streptomyces mobaraensis. Optimal conditions for maximal productivity were determined based on eight parameters at three different levels. The sources of carbon and nitrogen concentration and their...
August 2010
Identification of polymorphism in promoter region of growth hormone receptor (GHR) gene and its association with milk related traits in Holstein cows
It is widely accepted that hormones, growth factors and other agents exert their biological effects on target tissues by binding to specific receptors on the plasma membrane. The variability in constituent sequences of growth hormone receptor (GHR) gene is very important because of its major role in mammary gland development. The aim of the present study is to detect polymorphism in promoter region of GHR gene and its...
August 2010
Effects of different corn silage: Alfalfa silage ratios and full fat extruded soybeans on milk composition, conjugated linoleic acids content in milk fat and performance of dairy cows
The objective of this study was to investigate the effects of different corn silage : alfalfa silage ratios and full fat extruded soybeans on milk composition, especially that of conjugated linoleic acids content in milk fat and performance of dairy cows. Sixty Holstein cows were divided into 6 groups for a 12 week study. Treatments were arranged in a 2 × 3 factorial with 0 or 5% full fat extruded soybeans meal...
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