December 2012
Investigation of genetic effect of growth hormone gene on antler production in Chinese Sika deer Cervus nippon
Antler is one of the most important products of Sika deer (Cervus nippon) and has great economic values. The genetic mechanisms and genetic markers associated with antler growth of Sika deer have been rarely reported. In the present study, the growth hormone (GH) gene was analyzed as a candidate gene for antler growth in Chinese Sika deer. Partial sequence of GH exon3 and 5 of Sika deer were obtained and four...
December 2012
Characterization of Metarhizium anisopliae using amplifed ribossomal DNA restriction analysis (ARDRA) and internal transcribed spacer (ITS) sequence analysis
Molecular methods have revolutionized systematic entomology in the genusMetarhizium. In Brazil, the importance of these entomopathogenic fungi in agriculture is critical to the control of the sugar cane spittlebug (Mahanarva posticata) (Homoptera: Cercopidae). Other important factors for the selection of isolates ofMetarhizium anisopliae for use in the biological control of particular pests are...
December 2012
Optimization of the Clustal X program for the alignment of multicopper oxidase bacterial sequences and their presence in the Bacillus genus
Bioinformatic methods for the alignment of amino acid sequences and function prediction have to be robust, principally in conserved regions bound to enzymatic activity. In order to optimize amino acid sequence alignment in multicopper oxidase conserved sites of a collection of 103 bacterial sequences, modification were made in the weight values in different matrices for amino acid...
December 2012
Evaluation of denaturing gradient gel electrophoresis (DGGE) used to describe structure of bacterial communities in Istrian cheese
Denaturing gradient gel electrophoresis (DGGE) is a powerful method used to study structure of bacterial communities, without cultivation, based on the diversity of the genes coding for ribosomal RNA. However, the results are strongly dependent on the respective target region of the used primer systems. Therefore, three primer pairs that amplify different variable regions of the 16S rRNA gene...
December 2012
Two step culture for production of recombinant herpes simplex virus type 2 glycoprotein D in immobilized Spodoptera frugiperda cells
Herpes simplex virus type 2 (HSV-2) was the major cause of genital herpes in humans. The HSV-2 glycoprotein D (gD2) had been proved to be a potentially effective vaccine for treatment of genital herpes. The present study was to develop a two step culture to express the recombinant gD2 protein using the immobilized Spodoptera frugiperda (Sf9) cells. The first step, Sf9 cells were cultured and...
December 2012
Methylene blue (cationic dye) adsorption into Salvadora persica stems ash
Methylene blue (MB) is the most commonly used substance for dyeing cotton, wood and silk. On inhalation, it can cause difficulty in breathing, while on direct contact, it may cause permanent injury of the eyes of human and animals, burning sensations, nausea, worming, profuse sweating, mental confusion and methemoglobinemia. In the present study, Salvadora persica stem ash was used as an...
December 2012
Shoot and root morphogenesis from Eucalyptus grandis x urophylla callus
Eucalyptus grandis x urophylla plantlets were regenerated via indirect organogenesis. Histological assessment of their development focused on identifying the calli, the differentiation of shoots from the calli and the shoot-root junction from the nascent shoots. Vascular tissue formation within the callus preceded that of organised nodular structures, from which adventitious shoots were ultimately formed....
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