African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: 19 February, 2014; 13(8)

February 2014

Generation and characterization of pigment mutants of Chlamydomonas reinhardtii CC-124

The induced mutagenesis method for deriving pigment mutants of a green microalga, Chlamydomonas reinhardtii CC-124 and their pigment composition as well as ability to assess mutability of contaminated aquatic ecosystems were studied. In the present study, 14086 mutants (colonies) were obtained by exposure of the wild strain, C. reinhardtii CC-124, to 1, 2, 3, 5 min of ultraviolet (UV) irradiation. After screening, these...

Author(s): Zaydan, B. K., Sadvakasova, A. K., Saleh, M. M., and Gaballah, M. M.

February 2014

Genetic variability of European honey bee, Apis mellifera in mid hills, plains and tarai region of India

To observe the genetic variability in European honey bee, A. mellifera, PCR was run separately with five primers and analysis of the banding pattern was worked out to investigate the molecular profile of honey bee genotypes collected from different locations having random amplified polymorphic DNA (RAPD) primers. All the five primer screened, amplified the product in between the range of 100 to 1300 bp and 49 scorable...

Author(s): Kumar Yogesh and M. S. Khan

February 2014

Genetic diversity study of Kenyan cassava germplasm using simple sequence repeats

Cassava (Manihot esculenta Cranzt) is an important food security crop for resource poor rural communities particularly in Africa. Little is however known about molecular diversity of Kenyan cassava germplasm. This led to a study whose objective was to identify genetic constitution of cassava accessions from different regions of Kenya using molecular tools. Seven pairs of micro satellite (SSR) primers previously...

Author(s): John N. Ndung’u, Francis N. Wachira, Miriam G. Kinyua, David K. Lelgut, Peter Njau, Henry Okwaro and Hannington Obiero

February 2014

A comparative study of the use of tiger-specific and heterologous microsatellite markers for population genetic studies of the Bengal tiger (Panthera tigris tigris)

Comparison of genetic diversity indices of heterologous and species-specific microsatellite loci within a species may provide a panel of appropriate markers for genetic studies, but few studies have carried out such comparisons. We examined and compared the genetic characteristics of tiger-specific and heterologous loci in eight captive Bengal tigers. The mean polymorphic information content (PIC) value of the...

Author(s): Sudhanshu Mishra, Reeta Sharma, Sujeet Kumar Singh, Ashok Kumar Munjal, and Surendra Prakash Goyal

February 2014

Effects of moisture barrier and initial moisture content on the storage life of some horticultural produce in evaporative coolant

Shelf-life of five horticultural produce were studied. These include three leafy vegetables: Telfairia occidentalis, Celosia argentea and Amaranthus cruentus and two fruit vegetables: Lycopersicum esculentum and Abelmuschus esculentus. The layout plan of the experiment was a 3x2 factorial in a completely randomized design and each treatment replicated three times. The two factors examined were moisture barrier at three...

Author(s): Iwuagwu, Christian C. and N. J. Okonkwo

February 2014

The effect of mutagens on M1 population of black gram (Vigno mungo L. Hepper)

This study was carried out with black gram (urdbean) variety TNAUCo(Bg)6 to determine the effects of gamma rays (150, 200, 250, 300 and 350 Gy) and ethyl methane sulphonate (10, 15, 20, 25, 30 mM). Data collected were on seed germination and survival, pollen and seed fertility, plant height, number of primary branches, number of clusters per plant, number of pods per plant, pod length, number of seeds per plant, hundred...

Author(s): B. Ramya, G. Nallathambi and S. Ganesh Ram

February 2014

In vitro cytotoxic study for partially purified L-asparaginase from fresh leaves, unripe and ripe fruits of Withania somnifera plant

This work aimed to study the cytotoxic effect of L-asparaginase isolated from local Withania somnifera plant on lymphocyte leukemia cells. To achieve this goal, L-asparaginase was purified from W. somnifera fruits by two purification steps, ion-exchange chromatography using DEAE-cellulose and gel filtration chromatography using Sephadex G-150, and the study utilized an in vitro evaluation for the cytotoxic effect of the...

Author(s): Saad M. Nada, Najwa S. Ahmed, Yasameen Ali Hadi, Zeina Saif Al-Dein Mohammed and Zainib H. A. Al Rikabi

February 2014

Function of VtPGIP in pathogenic fungus resistance of Vitis thunbergii

In plants, polygalacturonase inhibitor proteins (PGIPs) are very important to inactivate polygalacturonases secreted by pathogens. Vitis thunbergii Sieb. et Zucc. polygalacturonase inhibitor proteins (VtPGIP) was first isolated from the wild grape Vitis thunbergii Sieb. et Zucc., which exhibits high resistance to disease. VtPGIP is sublocated in the plant cell wall, and this location is consistent with the function of...

Author(s): Yanhong Liu, Zhen Zhang, Xinwei Wang, Sanhong Wang, Huaizhi Jia, Hong Wang, Chang Dong, Jiyu Zhang and Jianmin Tao,

February 2014

Study on the extraction of dioscin by the ultrasonic-assisted ethanol

With Dioscorea zingiberensis as row materials, and with the yield of diosgenin as assessment criteria, the effect on extraction yield of dioscin of frequency of ultrasonic, the period of ultrasonic and solid-liquid ratio (D. zingiberensis : alcohol) was studied via orthogonal test. A new and unique method to accomplish this was by utilizing the technology of ultrasonic assisted ethanol extraction. The optimal processing...

Author(s): Li Xiang, Ma Jianzhong, Xia jing and Shi yundong

February 2014

Light microscopic detection of Plasmodium falciparum in vitro through Pf histidine rich protein 2 (HRP 2) gold conjugate labeling: Rapid diagnosis of cerebral malaria in humans

Plasmodium falciparum (Pf) has been found to be the deadliest of all the known species of the parasite capable of infecting humans; this is because it is capable of causing severe cerebral tissue damage. This study was carried out to demonstrate the parasite in the host blood in vitro through immunogold labeling using antibodies against Plasmodium falciparum histidine rich protein 2 (HRP 2); a major metabolite released...

Author(s): Ogundele Olalekan Michael, Saheed Sabiu, Adekeye Adeshina Oloruntoba, Adeniyi Philip Adeyemi, Ogedengbe Oluwatosin Olalekan, Enye Linus Anderson and Anosike Victor Ikenna

February 2014

Structure of vasa deferentia and spermatophores in Parapenaeopsis stylifera (H. Milne Edwards) (Decapoda: Penaeidae)

The structure of vasa deferentia and spermatophores has been described in Parapenaeopsis stylifera. The male reproductive system consists of two symmetrical halves; each half bears testis, vas deferens and an ejaculatory duct. Each testis comprised of two to three short, broad and milky white lobes; vas deferens is divisible into proximal, medial and distal parts. The proximal vas deferens (PVD) is a convoluted mass...

Author(s): Faiz Muhammad, Razia Sultana and Muhammad Shafi