African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 19 March, 2014; 13(12)

March 2014

Identification and utility of sequence related amplified polymorphism (SRAP) markers linked to bacterial wilt resistance genes in potato

Bacterial wilt caused by Ralstonia solanacearum is one of the most economically important diseases affecting potato (Solanum tuberosum). It is necessary to develop more molecular markers for potential use in potato genetic research. A highly resistant primitive cultivated species Solanum phureja was employed to generate a F1 mapping population to perform the bulked segregant analysis (BSA) for screening and identifying...

Author(s): Zhi Yanping, Li Hui, Zhang Hairui, and Gang Gao

March 2014

Allele frequency of hyperkalemic periodic paralysis (HYPP) in quarter horses from Mexico

Hyperkalemic periodic paralysis (HYPP) is an autosomal co-dominant genetic disease of Quarter-mile horses which originated by a point mutation of the gene coding the sodium channel protein in the plasmatic membrane of muscular cells. The mutation affects both dominant homozygous and heterozygous animals with myotonia, unpredictable muscular paralysis, weakness and collapse. In some cases, death can occur due to...

Author(s): Riojas-Valdés, V., Zamora-Ávila, D. E., Hernández-Escareño, J. J., Marroquín-Cardona, A., Picón-Rubio, F. J., Villarreal-Villarreal, J. P. and Ávalos-Ramírez, R.

March 2014

Transcriptional profiling of three key genes of terpenoid indole alkaloid pathway in Catharanthus roseus under different tissue culture conditions

The response of three key genes: strictosidine synthase (str1), tryptophan decarboxylase (tdc) and secologanin synthase (cyp72A1) of the wild plant species, Catharanthus roseus to different plant tissue culture treatments was studied. These genes encode enzymes acting early in the biosynthetic pathway of terpenoid indole alkaloids. In vitro culture system conditions involved the application of three sucrose (40, 50 and...

Author(s): Mona M. Moghazee, Fatma M. I. Badawy, S. Edris, I. S. Ibrahim, F. M. El-Ddomyati, and A. Bahieldin,

March 2014

Seasonal response of okra (Abelmoschus esculentus L. Moench) genotypes for okra yellow vein mosaic virus incidence

One of the major limiting factors of okra is the incidence of okra yellow vein mosaic virus (OYVMV), its vector being whitefly. Infection of 100% plants in a field is very common and yield losses range from 50 to 94% depending on the stage of crop growth at which infection occurs. A total of 91 okra genotypes were evaluated in summer and rainy season of 2012 to 2013, out of which only 6 genotypes (IIHR123, IC90381,...

Author(s): S. S. Solankey, Shirin Akhtar, Randhir Kumar, R. B. Verma and Kumar Sahajanand

March 2014

Analyses of soil cadmium and copper contents on a Domérien soil series of Burgundy in France

The aim of this study is to determine the availability of cadmium (Cd) in the soil of Yonne district, Burgundy, France. Soil samples were collected from surface-ploughed layer in fields across the southern part of the Yonne district, Burgundy, France. Some results analysing soil Cd and Cu contents on Domérien region of Burgundy has been presented. This is to know the relationship between extracting time and...

Author(s): Alain BERMOND, Denis BAIZE, Michel MENCH and Sangdeog A. KIM

March 2014

Identification of virus isolates inducing mosaic of sugarcane in Makarfi Local Government Area of Kaduna State, Nigeria

Sugarcane mosaic disease caused by sugarcane mosaic virus (SCMV), Johnsongrass mosaic virus (JGMV), maize dwarf mosaic virus (MDMV) and sorghum mosaic Virus (SrMV) is an economically important viral disease of sugarcane worldwide. Field survey was conducted to assess the presence of the viruses involve in mosaic disease of sugarcane in Makarfi Local Government Areas of Kaduna State (Northern Guinea Savannah), Nigeria. A...

Author(s): Dangora D. Balarabe, Yahaya Adama, Khan Azmat U U and Zangoma M. Aisha

March 2014

Protocol optimization for in vitro mass propagation of two sugarcane (Saccharum officinarum L.) clones grown in Ethiopia

The present study was initiated to optimize in vitro protocol for mass propagation of two commercial sugarcane clones (Co 449 and Co 678) grown in Ethiopia through shoot tip culture. Experiments on shoot multiplication and rooting were laid out in a completely randomized design with factorial treatment arrangements. Shoot tips were surface sterilized with 5% active chlorinated Berekina for 25 min and initiated...

Author(s): Tilahun Mekonnen, Mulugeta Diro, Manju Sharma and Tadesse Negi

March 2014

Fertigation of stillage in the culture of brown and golden linseed (Linum usitatissimum)

In Brazil, the stillage is mainly used in fertigation of sugarcane plantations, however, little is known about its effect on the nutritional supplementation and irrigation cultivation of linseed. Because of the shortage of work in the area, the present study aimed to evaluate the development of the culture of brown and golden linseed submitted to fertigation of stillage. The experiment was conducted in the field, at the...

Author(s): Paulo André Cremonez⊃, Armin Feiden⊃, Reginaldo Ferreira Santos⊃, Joel Gustavo Teleken⊃, Doglas Bassegio⊃, Filipe Eliazar Cremonez⊃, Henrique Bernardo Muriana⊃ and Willian Cézar Nadaleti⊃

March 2014

Effect of high doses of equine chorionic gonadotrophin (eCG) treatments on follicular developments, ovulation and pregnancy rate in boer goats

The aim of this study was to determine the effects of the superovulatory technique using equine chorionic gonadothrophin (eCG) on follicle response, ovulation and pregnancy rate in Boer goats. Twenty nine (29) does were divided into three groups, G1 (n = 11), G2 (n = 8) and G3 (control, n = 10). All groups had their estrus synchronized by the use of controlled internal drug release (CIDR) containing 0.3 g progesterone...

Author(s): Mohamad Shuib Mohamad Mohtar, Abd Wahid Haron, Rosnina Yusoff, Md Zuki Abu Bakar and Abdul Malik

March 2014

Inheritance of grain yield and its correlation with yield components in bread wheat (Triticum aestivum L.)

The genetic effect of grain yield plant-1 and its correlation with yield components were investigated in a 7 × 7 incomplete diallel cross of seven wheat parents during the crop season of 2009 to 2010. Mean square of general combining ability (GCA) effect was 2.90 for grain yield plant-1, which was highly significant (P<0.01), indicating that additive effect played important role in the inheritance of the trait....

Author(s): Jinbao Yao, Hongxiang Ma, Xueming Yang, G.uocai Yao and Miaoping Zhou

March 2014

Characterization and quantification of phenolic compounds of extra-virgin olive oils according to their blending proportions

Chemlali olive oil was blended with oils obtained from Oueslati and Chetoui varieties to improve the quality of the former one. Parameters such as triacylglycerols and phenolic compounds were characterized for various blends of Chemlali x Oueslati and Chemlali x Chetoui. Results show that blended oils had an improved composition as compared to that of Chemlali. In fact, the highest percentage of Oueslati and Chetoui...

Author(s): Youssef Ouni, Mokhtar Guerfel, Linda Ben Yahia and Mokhtar Zarrouk

March 2014

Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

In this work, we report the production of lipases by a Fusarium sp. isolate (FCLA-MA41) from Atlantic Forest, using chicken fat and association of organic and inorganic nitrogen sources in submerged fermentation to seek economically attractive bioprocess. A 2-level, 4-factor Central Composite Design (CCD) and response surface methodology (RSM) were used to study the influence of the concentrations of chicken fat,...

Author(s): Bruno H. Oliveira, and Valéria M.G. Lima,

March 2014

Synthesis of liposomes using α-phosphotidycholine and metabolites obtained from Elephantorrhiza elephantina and Pentanisia prunelloides

Plant saponins exhibit numerous pharmacological characteristics desirable for long term hyperlipidemic therapy through their cholesterol binding capacity due to the formation of liposomes/phytosomes which ultimately decreases the gastrointestinal absorption of cholesterol. This may result in the reduction of the blood plasma cholesterol levels; hence, mitigating cardiovascular and atherosclerotic problems that are...

Author(s): Smart J. Mpofu, Titus A. M. Msagati and Rui W. M. Krause

March 2014

Optimization of chromium biosorption in aqueous solution by marine yeast biomass of Yarrowia lipolytica using Doehlert experimental design

Optimization of a chromium biosorption process was performed by varying three independent variables pH (0.5 to 3.5), initial chromium ion concentration (10 to 30 mg/L), and Yarrowia lipolytica dosage (2 to 4 g/L) using a Doehlert experimental design (DD) involving response surface methodology (RSM). For the maximum biosorption of chromium ion in an aqueous solution by Y. lipolytica, a total of fifteen experimental runs...

Author(s): Sarat Babu Imandi, Ramakrishna Chinthala, Silas Saka, Rama Rao Vechalapu and Kiran Kumar Nalla

March 2014

Role of c-Src inhibitor in the regulation of hepatocarcinoma cell migration

It has been discovered that hepatocellular carcinoma (HCC) has high ability of migration and angiogenesis. This study aimed to explore the mechanism of HCC cell migration and angiogenesis. BEL-7402 cell line was used as HCC cell model for investigating the regulation of cell migration upon c-Src inhibitors (PP2 and SU6656) treatment. Western blot was used for detecting the expression of MT1-MMP and VEGF-C. The activity...

Author(s): Hui-min Liang, Yao-zhou Zhang, Zhen-kun He, Qing-jie Xu and Xiao-yan Shi

March 2014

Insecticidal activity of bioproducts on Ceratitis capitata Wiedemann (Diptera: Tephritidae)

The aim of this study was to evaluate the insecticidal activity of the bioproducts proagrim, essential oil from fennel and orange oil, on the mortality of Ceratitis capitata Wiedemann (Diptera: Tephritidae). The study was divided into three parts: the first assay studied the insecticidal activity of the products on infested fruit; secondly, a bioassay was performed by ingestion of the bioproducts in an artificial diet,...

Author(s): Flávia Queiroz de Oliveira, José Bruno Malaquias, Wennia Rafaelly de Souza Figueiredo, Jacinto de Luna Batista, Eduardo Barbosa Beserra and Robério de Oliveira

March 2014

Effect of cooling on sperm motility before and after frozen-thawed stallion semen

The aim of this study was to assess the effect of cooling on sperm motility before and after frozen-thawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen was frozen-thawed....

Author(s): R. A. Ledezma-Torres, L. Sandoval, P. Valladares, M. Mellado, F. J. Picón, R. Ramírez and F. Sánchez