African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12275

Table of Content: 1 April 2015; 14(13)

April 2015

GC-MS analysis of pesticidal essential oils from four Kenyan plants

Essential oils are complex mixtures of odours and steam volatile compounds which are deposited by plants in the subcuticular space of glandular hairs, in excretory cavities and canals or exceptionally in heart wood. Essential oils have been found to have no specific biological functions in plants, but constitute many compounds that are insect repellents or act to alter insect feeding behavior, growth and development,...

Author(s): Gladys Kemunto Onyambu, Rosebellah Maranga, Mary Ndungu, Gerald M. Mkoji,Kareru P. G. and Anthony Wanjoya

April 2015

A comparison of nrDNA internal transcribed spacer (ITS) loci for phylogenetic inference and authentication among Cinnamomum osmophloeum and related species in Taiwan

Cinnamomum osmophloeum, an indigenous species of Taiwan, can be utilized for valuable products such as a food, a spice and a traditional Chinese medicine. This study compares the ribosomal DNA (nr DNA) internal transcribed spacer (ITS) sequence of C. osmophloeum to that of several other species with similar external morphology, such as Cinnamomum burmannii, Cinnamomum insularimontanum, Cinnamomum macrostemon and...

Author(s): Kuen-Yih HO, Shih-Chieh LEE, Shr-Wei SHIUE and Chung-Chi HWANG

April 2015

Relative efficiency of RAPD and ISSR markers in assessment of DNA polymorphism and genetic diversity among Pseudomonas strains

This study presents the results from comparison of random amplified polymorphic DNA (RAPD), random amplified polymorphic DNA (RAPD) and RAPD-ISSR markers together in determining the genetic diversity and polymorphism among strains of fluorescent Pseudomonas. In order to compare the efficiencies of these markers, 17 different isolates were investigated using RAPD and ISSR. In RAPD marker analysis, 147 out of 222 bands...

Author(s): Jaipreet Kaur Rayar, Mohammad Arif and Uma Shankar Singh,

April 2015

Genetic diversity of Przewalski’s gazelle using noninvasive DNA and its implications for conservation

The Przewalski’s gazelle (Procapra przewalskii) is endemic to China and the total current population of the species is only about several hundred. In order to understand the genetic structure and diversity of the Przewalski’s gazelle in China for the purpose of guiding conservation initiatives, we examined the genetic variability and differentiation based on microsatellite loci, using noninvasive genetic...

Author(s): Hairui Duo, Li Na, Yanyun Hong,Yuguang Zhang and Diqiang Li

April 2015

Effect of recombinant bovine somatotropin application intervals on Girolando cows’ milk production and composition

The aim of this study was to evaluate the effect of recombinant bovine somatotropin (rBST) application intervals on chemical composition of milk from Girolando cows with productivity below 20 L/milk/day and animals with productivity above 20.1 liters/milk/day. The study included 30 Girolando cows with production ranging from 13 to 28 L/milk/day. Cows were submitted to two milkings: 06:00 am and 04:00 pm. Milk samples...

Author(s): Thiago S. Carvalho, Marco Antônio P. da Silva, Jakeline F. Cabral, Rafaella B. Brasil, Lígia C. de Moura, Cristiane I. Giovannini and Rodrigo Balduíno S. Neves.

April 2015

Efficient micropropagation of Epidendrum secundum Jacq. from leaves and protocorms

An efficient method for the large-scale propagation of Epidendrum secundum using protocorms and leaves from in vitro germination was established. Explants were inoculated in Woody Plant Medium (WPM) supplemented with 6-benzyladenine (BA) (1.0–40.0 µM) for 60 days and cultured in a growth room in darkness (30 days) or with a 16 h photoperiod. Protocorms were most effective for regenerating protocorm-like...

Author(s): Danielle Lopes Ferreira, Eric de Camargo Smidt and Luciana Lopes Fortes Ribas

April 2015

In vitro regeneration and induction of multiple shooting in Cicer arietinum L. using cotyledonary nodal explants

Chickpea, a temperate crop, is the world’s third most important pulse crop and India produces 75% of world’s supply. The two most common types of Chickpea are the white-seeded "Kabuli" and the "Desi "variety. In vitro regeneration was achieved using cotyledonary nodal explants using desi variety seeds of cultivar K850 of Cicer arietinum. The multiple shoot induction was observed with...

Author(s): Sruthi Prema Sunil, J. Philip Robinson, S. S. KarthickBalan, M. Anandhaprabhakaran and V. Balakrishnan

April 2015

In-vitro evaluation of plant extracts against Lasiodiplodia theobromae causing cashew inflorescent blight

Extracts of four plant species, red acalypha (Acalypha hispida), siam weed (Chromolaena odorata), aidan (Tetrapleura tetraptera), and neem (Azadirachta indica) were screened in-vitro for fungitoxicity against Lasiodiplodia theobromae (cashew inflorescence blight pathogen) at four different concentrations viz., 10, 20, 40 and 80%. The result shows that the plants extracts significantly (P < 0.05) reduced mycelia...

Author(s): Dele Omoyele Adeniyi and Abiodun Joseph

April 2015

Biosorption of hexavalent chromium (chromium (VI) ion from industrial tannery effluent using filamentous fungi on modified rice husk medium/potato dextrose medium (MRHM/PDM)

An experimental effluent analysis was conducted in conjunction with bioremediation process from Mario-jose tannery industry, stream and reference water samples from well (Yandoka), respectively, were collected from the industrial areas. Physiochemical characteristic of these samples were determined viz; chemical oxygen demand (COD), biochemical oxygen demand (BOD), pH, temperature etc. The effluent values were mostly...

Author(s): S. D. Emmanuel, I. K. Adamu, A. Ejila, M. I. Ja’afaru, Y. Amos, M. Abubakar and O. Agbor

April 2015

Phytotoxicity of cadmium on peroxidation, superoxide dismutase, catalase and peroxidase activities in growing peanut (Arachis hypogaea L.)

A pot experiment treated with cadmium (Cd) was conducted to evaluate the physiological and yield responses of peanut to cadmium in different growth stages. The results indicated that the peanut treated with cadmium level of 12 mg/kg did not cause obvious visible toxic symptoms, while the antioxidant enzymes activities concluding superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) showed significant decrease in...

Author(s): Chun-juan Li, Cai-xia Yan, Yu Liu,Ting-ting Zhang, Shu-bo Wan and Shi-hua Shan

April 2015

Potential proliferative effect of lipopolysaccharide preconditioning on human umbilical cord blood-derived endothelial progenitor cells

Endothelial progenitors cells (EPCs) are important for the development of cell therapies for various diseases. However, the major obstacles in developing such therapies are low quantities of EPCs that can be generated from the patient and the lack of adequate non-invasive imaging approach for in vivo monitoring of transplanted cells. Bacterial lipopolysaccharide (LPS) is a key mediator in the vascular leak syndromes...

Author(s): Sahar H. Ahmed, Dina Sabry, Olfat Noh and Mai Samir