African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 1 June 2011; 10(23)

June 2011

The trends and future of biotechnology crops for insect pest control

Biotech crops, including those that are genetically modified (GM) with Bacillus thuringiensis (Bt) endotoxins for insect resistance, have been cultivated commercially and adopted in steadily increasing numbers of countries over the past 14 years. This review discusses the current status of insect resistant transgenic crops and the often raised concern that its resilience is limited and that its efficacy will...

Author(s): Santie M. DeVilliers and David A. Hoisington          

June 2011

Experiences with the biosafety regulatory system in Kenya during the introduction, testing and development of Bt maize

The introduction of genetically modified (GM) crops in Kenya is governed by a biotechnology policy, a biosafety law, and a biotechnology awareness strategy to enable research and development of GM crops. In addition, Kenya has the National Biosafety Authority (NBA) that regulates plant biosafety through technical institutions including the Kenya Plant Health Inspectorate Services (KEPHIS). Bacillus...

Author(s): Stephen Mugo, Simon Gichuki, Murenga Mwimali, CatherineTaracha, and Harrison Macharia      

June 2011

Experiences in effective communication on transgenic technology in Africa – the case of the insect resistant maize for Africa (IRMA) project

The Insect Resistant Maize for Africa (IRMA) Project, aimed to improve food security through developing and deploying locally adapted stem borer resistant maize varieties using both conventional and biotechnology mediated methods, especially Bt technology. This technology uses a gene from the soil bacterium Bacillus thuringiensis (Bt) to create transgenic maize varieties. Transgenic technologies have been a...

Author(s): A. Wangalachi, D. Poland, S. Mugo, S. T. Gichuki, D. Ouya, G. Kimaniand J. Rabar      

June 2011

Options for pro-poor maize seed market segmentation in Kenya

New agricultural technologies have to be affordable to make a difference in poor farmers’ livelihoods. Their cost to the poor can be reduced through subsidies by the public sector or humanitarian use exemption from Intellectual Property Rights (IPR) by the private sector. Either option needs market segmentation, common in the health sector, but not in agriculture. This paper analyzes options for pro-poor market...

Author(s):   Hugo De Groote, Michael D. Hall, David J. Spielman, Stephen N. Mugo, Kwaw Andam, Bernard G. Munyua, and Marianne Banziger

June 2011

Testing public Bt maize events for control of stem borers in the first confined field trials in Kenya

Transgenic  maize (Zea mays L), developed using modified genes from the bacteriumBacillus thuringiensis (Bt), controls stem borers without observable negative effects to humans, livestock or the environment, and is now sown on 134 million hectares globally. Bt maize could contribute to increasing maize production in Kenya. Nine public Bt maize events of cry1Ab and cry1Ba genes were...

Author(s): Stephen N. Mugo, Murenga Mwimali, Catherine O. Taracha, Josephine M. Songa, Simon T. Gichuki, Regina Tende, Haron Karaya, David J. Bergvinson, Allesandro Pellegrineschi, and David A. Hoisington

June 2011

Control of Busseola fusca and Chilo partellus stem borers by Bacillus thuringiensis (Bt)-δ-endotoxins from Cry1Ab gene Event MON810 in greenhouse containment trials

Previous testing of several public Bacillus thuringiensis (Bt)-maize events did not show control of the African stem borer (Busseola fusca Fuller), an important stem borer species, without which stewardship would be compromised by the possibility of rapid development of resistance to Bt delta-endotoxins. This study was carried out to test Bt-maize Event MON810 as an option to control all major stem borer...

Author(s): Stephen Mugo, Mwimali G. Murenga, Haron Karaya, Regina Tende, Catherine Taracha, Simon Gichuki, Jane Ininda, Kinyua M’bijjewe, and Andrew Chavangi      

June 2011

Levels of control of Chilo partellus stem borer in segregating tropical Bt maize populations in Kenya

In Kenya, stem borers destroy an estimated 400,000 metric tons, or 13.5%, of farmers' annual maize harvest costing about US$80 millions. Bacillus thuringiensis(Bt) maize controls stem borers without harming humans, livestock and the environment and was sown to 140m ha-1 globally in 2009. Two public Bt maize lines of cry1Ab::ubi gene (Event 216 and Event 223) were crossed with two non-Bt maize...

Author(s): Mwimali G. Murenga, Stephen M. Githiri, Stephen N. Mugo and Florence M. Olubayo        

June 2011

Evaluation of stem borer resistance management strategies for Bt maize in Kenya based on alternative host refugia

Stem borers are the major insect pests of maize in Kenya. The use of Bacillus thuringiensis (Bt) technology is an effective way of controlling lepidopteran pests.  However, the likelihood of development of resistance to the Bt toxins by the target stem borer species is a concern. Forages, sorghum and maize varieties were evaluated for stem borer preference and survivorship in the laboratory and field in...

Author(s): Margaret M. Mulaa, David J. Bergvinson, Stephen N. Mugo, Japhether M. Wanyama, Regina M. Tende, Hugo De Groote and Tadele M. Tefera          

June 2011

Assessing the potential economic impact of Bacillus thuringiensis (Bt) maize in Kenya

The Insect Resistant Maize for Africa (IRMA) project is currently developing Bt maize for Kenya. So far, Bt genes with resistance to Chilo partellus, Chilo orichalcociliellus,Eldana sacharina, and Sesamia calamistis, four of the five major stemborers were successfully incorporated into elite CIMMYT maize inbred line (CML216) and tested in insect bioassays in Kenya. Participatory Rural Appraisals...

Author(s): Hugo De Groote, William A. Overholt, James O. Ouma, and J. Wanyama          

June 2011

Genotype by environment interactions and yield stability of stem borer resistant maize hybrids in Kenya

In a maize breeding program, potential genotypes are usually evaluated in different environments before desirable ones are selected. Genotype x environment (G x E) interaction is associated with the differential performance of genotypes tested at different locations and in different years, and influences selection and recommendation of cultivars. Twenty one stem borer resistance maize hybrids and four commercial checks...

Author(s): Yoseph Beyene, Stephen Mugo, Charles Mutinda, Tadele Tefera, Haron Karaya, Sammy Ajanga, Jackson Shuma, Regina Tende and Vincent Kega      

June 2011

Combining ability of maize (Zea mays L.) inbred lines resistant to stem borers

Ten inbred parents with varying resistance levels to Chilo partellus and Busseola fusca were crossed in a half diallel mating scheme to generate 45 F1 hybrids. The hybrids and five commercial checks were evaluated across four locations in Kenya under artificial and natural infestation in 2009. Genotype (G) by environment (E) interaction (G x E) was non-significant for stem borer leaf damage, number...

Author(s): Yoseph Beyene, Stephen Mugo, John Gakunga, Haron Karaya, Charles Mutinda, Tadele Tefera, Stephen Njoka, Dorcas Chepkesis, Jackson M. Shuma and Regina Tende        

June 2011

Farmers, consumers and gatekeepers and their attitudes towards biotechnology

In 1999, a project to develop insect resistance maize for Africa was launched. Social scientists from this team used participatory rural appraisals, consumer studies, a baseline and gatekeeper survey to study the awareness and attitudes towards biotechnology among farmers, consumers and gatekeepers. Farmers’ awareness of biotechnology was very low (12.7%). Awareness on genetically modified (GM) crops among...

Author(s): Simon C. Kimenju, Hugo De Groote, Charles Bett, and Japhether Wanyama      

June 2011

Grain yield, stem borer and disease resistance of new maize hybrids in Kenya

Evaluation of 30 maize hybrids for yield and resistance to stem borers and foliar diseases in four agro-ecologies in Kenya was conducted in 2009. There were significant differences among the hybrids in leaf damage, number of exit holes, tunnel length and grain yield in Kiboko. The maize hybrids CKPH08014, CKPH08025, and CKPH08026 showed the least leaf damage, exit holes and tunnel length, similar to the resistant...

Author(s): Tadele Tefera, Stephen Mugo, Yoseph Beyene, Haron Karaya and Regina Tende