July 2011
Determination methods of cellulase activity
There are lots of assay methods used for cellulase activity that have not been unified. According to their experimental determination purpose, many research institutions adopt different methods, and do some changes according to their own experience, making determination to be more diversified. Summarizing the determination methods of cellulase activity and problems, it can provide references and assistance for the...
July 2011
Genetic analysis of yield in peanut (Arachis hypogaea L.) using mixed model of major gene plus polygene
A recombinant inbred line (RIL) population with 215 lines derived from a cross between a highly yield line Zheng8903 (female parent) and a variety Yuhua No.4 (male parent) was used in this research. The RILs population was grown in three environments to make a genetics analysis of yield from F7 to F9. Jointly segregating analysis was used to analyze the genetics of yield in the combination of Zheng8903×Yuhua...
July 2011
Genetic variation in hawthorn (Crataegus spp.) using RAPD markers
In this study, we report the use of random amplified polymorphic DNA (RAPD) to determine genetic relationships in the genus Crataegus. Five species, includingCrataegus monogyna, Crataegus meyeri, Crataegus aronia, Crataegus pentagynaand Crataegus pontica were chosen from northwest forests of Iran and analyzed. Nine RAPD primers reproducibly and strongly generated 101 discrete markers. Seventy...
July 2011
Endothelial protein C receptor in renal tubular epithelial cells and influencing factors
The endothelial protein C receptor (EPCR) plays an important role within the protein C pathway in regulating coagulation and inflammation. It was reported that EPCR was expressed in large vessels, placenta, heart, liver and lung endothelial cell. However, there are a few studies concerned about renal epithelial cells. This study aims to investigate EPCR expression in renal tubular epithelial cells and related...
July 2011
Isolation of a novel PP2C gene from rice and its response to abiotic stresses
Biotic and abiotic stresses adversely affect plant growth and improvement. In order to discover new stress-tolerance genes in rice (Oryza sativa L.), we analyzed a global genome expression profiling of the indica cultivar Pei’ai 64S, using Affymetrix rice expression chip, subjected to cold, drought or heat stress. A large number of genes highly up regulated or down regulated were...
July 2011
Cloning and expression analysis of a blue copper-binding protein gene from Dasypyrum Villosum
A differentially expressed fragment EST145 was isolated by suppression subtractive hybridization (SSH) method. Using EST145 as the probe, a blue copper-binding protein gene designated as DvBCB was screened from Dasypyrum villosum cDNA Library. The DvBCB gene was 845 bp in length with an open reading frame (ORF) which encoded a 178-amino acid polypeptide and contained the deduced functional...
July 2011
A method for high-quality RNA extraction from tall fescue
The isolation of high-quality RNA was a precondition in molecular biology research of tall fescue. Two common approaches were adopted for the total RNA extraction by using leaves of tall fescue as the material in this experiment in order to seek the optimized total RNA extraction method of tall fescue, as well as the improvement of the extraction method used by the predecessors. The results showed that the Trizol method...
July 2011
Increased gum arabic production after infestation of Acacia senegal with Aspergillus flavus and Pseudomonas pseudoalcaligenes transmitted by Agrilus nubeculosus
The aim of this study was to investigate the correlation between the beetle Agrilus nubeculosus and gum arabic production by Acacia senegal. Some trees were tapped and left open to facilitate infestation by A. nubeculosus and others were covered with wire mesh as control. Gum yield, physical and chemical properties of gum were determined for infested and control trees. A. senegal infested by A. nubeculosus produced...
July 2011
Comparative evaluation of organic formulations of Pseudomonas fluorescens based biopesticides and their application in the management of bacterial wilt of brinjal (Solanum melongena L.)
An experiment was conducted in the laboratory and farm of the Department of Biotechnology, Gauhati University, to explore the potentiality of various organic formulations of Pseudomonas fluorescens (Pf) and to manage bacterial wilt disease of brinjal (Solanum melongena L.) under local conditions. Different organic materials and adhesives were used in the formulation of Pf based biopesticides and stored at...
July 2011
Growth and physiological response of tall oat grass to salinity stress
In order to evaluate the responses of tall oat grass plants to increasing salinity, we measured plant growth, ion contents, photosynthetic gas exchange, lipid peroxidation, and proline accumulation in four salt concentrations. Two tall oatgrass genotypes, ZXY03P-367 and ZXY03P-443, were grown for 14 days in greenhouse conditions and after 14 days treated with four NaCl treatments (0, 65, 100, and 135...
July 2011
Isolation and biochemical characterizations of the bacteria (Acidovorax avenae subsp. avenae) associated with red stripe disease of sugarcane
Studies on Acidovorax avenae subsp. avenae, associated with red stripe disease of sugarcane was conducted in the Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi during 2009 to 2010, in collaboration with Shakarganj Sugar Research Institute (SSRI), Jhang, Pakistan. Red stripe of sugarcane were recently observed on promising clones of sugarcane planted in autumn...
July 2011
Effect of cadmium hyperaccumulation on antioxidative defense and proline accumulation of Solanum nigrum L.
Changes in cadmium (Cd) accumulation, the activity of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and the concentrations of malondialdehyde (MDA), chlorophyll and free proline in Solanum nigrum, Cd-hyperaccumulator were examined and compared with a non-hyperaccumulator, Solanum lycopersicum L. It was indicated that the root and leaf SOD, POD...
July 2011
Bioreduction of Cr (VI) by potent novel chromate resistant alkaliphilic Bacillus sp. strain KSUCr5 isolated from hypersaline Soda lakes
Isolation of Cr (VI) resistant alkaliphilic bacteria from sediment and water samples collected from Wadi Natrun hypersaline Soda lakes (located in northern Egypt), resulted in isolation of several alkaliphilic bacterial strains that can tolerate up to 2.94 g/l of Cr (VI) in alkaline medium. However, with increasing Cr (VI) concentration up to 29.4 g/l, only one strain, KSUCr5, was able to tolerate up to 22 g/l (75 mM)...
July 2011
Novel approach for optimization of fermentative condition for polyhydroxybutyrate (PHB) production by Alcaligenes sp. using Taguchi (DOE) methodology
Polyhydroxybutyrate (PHB) is a biodegradable thermoplastic polymer which is accumulated as energy reserve material by large number of microorganisms including bacteria, fungus and yeast under nutrient stress condition. In this study, efforts have been made to optimize PHB production by Alcaligenes sp. NCIM 5085 using Taguchi (DOE) methodology. This approach facilitates the study of interaction of a large...
July 2011
Optimization of fermentation medium for enhanced production of milbemycin by a mutant of Streptomyces bingchenggensis BC-X-1 using response surface methodology
A new strain Streptomyces bingchenggensis BC-X-1, in which one of the impute was disappeared had been screened by N-methyl-N’-nitroso-N-nitroso-guanidine (NTG) mutation. In order to improve the yield of milbemycin by the new strain, response surface methodology was employed to optimize the composition of fermentation medium. The Plackett-Burman design indicated that yeast extract, soybean flour, KH2PO4,...
July 2011
Improved keratinase production for feather degradation by Bacillus licheniformis ZJUEL31410 in submerged cultivation
Optimal medium was used to improve the production of keratinase by Bacillus licheniformis ZJUEL31410, which has a promising application in the transformation of feather into soluble protein. The results of single factor design revealed that the concentration of feather at 20 g/l and the initial pH at value 8 was the best for the production of keratinase and the degradation of feather. Ammonia salt and nitrate...
July 2011
The effect of Camelina sativa cake diet supplementation on sensory and volatile profiles of ewe’s milk
The aim of this study was to evaluate the sensory profile based on the principal component analysis (PCA) and cluster analysis of Euclidean distances as well as evaluate a volatile profile in ewes’ milk. The analysis was conducted using SPME GC/TOFMS. Tested milk came from ewes fed concentrate supplemented with 10 and 20% Camelina sativa (L.) Crantz cake (CSC). This plant containing unsaturated fatty...
July 2011
Distinct physicochemical characteristics of different beef from Qinchuan cattle carcass
A total of 30 Qinchuan cattle were used to investigate the physicochemical characteristics of beef from three different parts of cattle carcass, namely longissimus dorsi (LD), serratus ventralis (SV) and semitendinosus (ST). Multiple parameters were ranked in the descending order of ST > SV > LD, and include muscle pH, protein, ash, drip loss, cooking loss, shear force and hydroxyproline (Hyp). In contrast, water,...
July 2011
Antiproliferative activity of recombinant human interferon-λ2 expressed in stably transformed BmN cells
This study aimed at the generation of a stable transformed silkworm BmN cell line which can continuously express human interferon-λ2 (IFN-λ2) gene, and investigated the antiproliferative activity of this recombinant human IFN-λ2. Silkworm BmN cells were transfected with the recombinant vector pIZT/V5-His harboring the human IFN-λ2 gene. After the BmN cells were transfected with the...
July 2011
Effect of vitamin C on salivary superoxide dismutase activity in smokers
This study was performed to elucidate the effect of ascorbic acid on salivary superoxide dismutase (SOD) activity in smokers. In this single blind, cross over clinical trial, whole unstimulated saliva of 30 smokers, who were randomly divided into two groups, was collected. In the first phase, patients of one group took 500 mg of vitamin C powder, for 3 weeks. Then saliva of all patients was...
July 2011
Effects of recombinant human collagen VI from Escherichia coli on UVA-irradiated human skin fibroblasts cells
In this study, we reported the cloning and over expression of a gene coding for human collagen peptide (CP6) in Escherichia coli and investigated the protective effects of CP6 on UVA-irradiated human skin fibroblasts cells. The collagen peptide (CP6) was highly soluble and the expression level was approximately 10% of the total bacteria proteins. Also, we performed assays with...
July 2011
Anticancer potential evoked by Pleurotus florida and Calocybe indica using T24 urinary bladder cancer cell line
Cancer is the second largest non-communicable disease and it has a sizable contribution in the total number of deaths. Cancer of the urinary bladder ranks fourth in men and eighth in women with an alarming increase in cancer patients of this type every year. Tobacco smoking is considered to be one of the causes of bladder cancer and a number of organic chemicals used in the industry are known to be...
July 2011
Effectiveness of carnosine on disturbed electrolytes homeostasis induced by cisplatin
We aimed to assess the effect of well known antioxidant carnosine on disturbed plasma and intra-erythrocytes electrolytes and Na+-K+-ATPase activity by cisplatin. 24 male albino Wistar rats were selected and divided into 4 groups: Group I = untreated control; Group II = cisplatin control (received cisplatin at a dose of 3 mg/ kg body weight; i.p. for 13 alternate days); Group III = carnosine alone...
July 2011
Bycatch in 36 and 40 mm PA Turkish twin rigged beam trawl codends
The Turkish twin rigged beam trawl which has two identical nets rigged side by side on the same beam targets mainly the Parapenaeus longirostris, although some bycatch fish species have also become target species. Thus, landings of the shrimp beam trawling fleet also include some commercially valuable fishes. Our study focused on the bycatch commercial species which are caught by the beam trawl fishery...
July 2011
Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India
Artemisia which produces a large number of secondary metabolites is naturally found in cold desert high altitude environment of India. Secondary metabolites such as alkaloids, flavonoids, phenols, polysaccharides and terpenes represent a significant barrier to the extraction of pure genomic DNA. Thus, in this study, the DNA extraction protocol to extract pure genomic DNA from...
July 2011
The relationships of phenotype, genotype and some environmental factors with birth weight in Jersey calves
This study investigated the effects of parity, birth type, gender and birth season on birth weight in Jersey cattle and also investigated the relationships of phenotype and genotype with birth weight. Birth records of the Karakoy farm near Samsun, Turkey for the period from 1998 to 2005 were used as data for this study. Parity (P < 0.001), birth type (P < 0.001) and gender (P < 0.001) had a significant effect...
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