December 2011
A combinatorial enumeration problem of RNA secondary structures
The total number of the RNA secondary structures with a limited length m in each hairpin loop, that is, Sm(n) has already been studied. In this paper, the number of this kind of RNA secondary structures is discussed with the technique of combinatorics. As a result, the recurrence relations, generating functions, explicit expressions, and some other properties were obtained....
December 2011
Low genetic diversity and genetic differentiation among domesticated stocks of the small abalone Haliotis diversicolor in China
AFLP markers were used to investigate levels of genetic diversity within cultured populations of the small abalone Haliotis diversicolor in China and to compare them with a wild population. A total of 234 fragments, with a size range of 150-500 bp, were identified from 152 individuals by five AFLP primer combinations. High proportion of polymorphic loci (77.65%) and heterozygosity (0.2124) were found...
December 2011
A new DNA band display technology of microsatellite DNA
This study proposes a new DNA band display technology of microsatellite DNA called Fluorescent Imaging Technology. In comparison with Silver Stain Technology, this technology is worth popularizing in the laboratory because of its high resolution, efficiency, simplicity and clear background. Key words: Microsatellite DNA, polyacrylamide gel electrophoresis, fluorescent imaging technology,...
December 2011
Myogenic conversion of bladder fibroblasts by construction and expression of eukaryotic expression vector of myod1 gene
Gene therapy of detrusor underactivity, by autologous cells transplantation, is limited by the number of primary myogenic. The purpose of this study was to investigate whether Myod1 could induce primary bladder fibroblasts to undergo myogenic conversion. Primary bladder fibroblasts from Sprague-Daley rats were cultured. The eukaryotic expression plasmid pEGFP-Myod1 carrying both a rat Myod1 cDNA and a green...
December 2011
Molecular cloning and characterization of pathogenesis-related protein 5 in Zea mays and its antifungal activity against Rhizoctonia solani
We described the cloning and characterization of pathogenesis-related protein 5 gene in maize, named ZmPR5 (GenBank Accession Number: HM230665). Molecular and bioinformatic analyses of ZmPR5 revealed an open reading frame (ORF) of 525 bp, encoding a protein of 175 amino acids (aa) and a deduced molecular mass of 17.5 KDa. Homology analysis of the Zea mays L. deduced amino acid sequence,...
December 2011
Four new single nucleotide polymorphisms (SNPs) of toll-like receptor 7 gene discovered in Chinese ducks
In order to reveal the single nucleotide polymorphisms (SNPs), genotypes and allelic frequencies of each mutation site of TLR7 gene in Chinese native duck breeds, SNPs of duck TLR7 gene were detected by DNA sequencing. The genotypes of 465 native ducks from eight key protected duck breeds were determined by polymerase chain reaction ligase detection reaction (PCR-LDR) method. The results show that four new...
December 2011
Polymerase chain reaction (PCR) detection of the predominant microcystin-producing genotype of cyanobacteria in Mozambican lakes
Mozambique is a developing country with a wide range of aquatic ecosystems. Given the limited resources of the country, problems of aquatic pollution have not received the required consideration. The aim of the present study was to assess the presence of microcystins (MCs) and identify the genotypes of MC-producing cyanobacteria in Mozambique. Polymerase chain reaction (PCR) based detection methods were used to analyze...
December 2011
Location effect on heritability estimates of yield traits in mungbean derived from F2 populations
Heritability and genetic advance were estimated in four F2 mungbean populations (Ramzan×ML-5, NM-92×NM-1919, NM-1919×ML-5 and ML-5×NM-51) at two locations (Peshawar and Swat) of Khyber Pakhtunkhwa province, Pakistan, during 2008.Combined analysis exhibited highly significant differences among the genotypes and locations for pods plant-1, pod length, seeds pod-1, 1000-seed weight and seed...
December 2011
Differentially expressed genes in white egg 2 mutant of silkworm, Bombyx mori, at early embryo development stages
White egg 2 is one of white egg mutants in silkworm, whose molecular mechanism remains unknown so far. In order to obtain an overall view on gene expression profiles at early embryo development stages, the white egg 2 near-isogenic line was constructed and the whole-genome of silkworm microarray system containing 21375 predicted genes from the silkworm whole genome sequence was employed to investigate gene expression...
December 2011
Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of spine gourd (Momordica dioica Roxb. ex Willd)
Spine gourd (Momordica dioica Roxb. ex Willd) is a medicinally and economically important plant and also used as vegetable. In this study, we established anAgrobacterium tumefaciens-mediated transformation procedure for M. dioica. Leaf explants were incubated with A. tumefaciens strain LBA4404 containing a binary vectorpBAL2 carrying the reporter gene β-glucuronidase intron...
December 2011
Shear stress and interleukin-8 (IL-8) on the proliferation, differentiation and tube formation of endothelial progenitor cells (EPCs)
Endothelial progenitor cells (EPCs) derived from bone marrow, are also found in circulation and involved in both tumor vasculogenesis and wound healing. During the process of EPCs incorporation into tissues and neovascularization, the cells are exposed to fluid shear stress. Interleukin-8 (IL-8) has been shown to play an important role in tumor growth, angiogenesis and metastasis. In our previous study, IL-8...
December 2011
Assessing genetic diversity of perennial ryegrass (Lolium perenne L.) from four continents by inter-simple sequence repeat (ISSR) markers
In this study, inter-simple sequence repeat (ISSR) markers were used to compare genetic diversity between commercial cultivars and natural germplasm which were obtained from Europe, Africa, Asia, and North America. There was a relatively high genetic variation in the whole collection judged by the polymorphism rate (97.16%), Nei's gene diversity (0.28), and Shannon's information index...
December 2011
Nursery growing of some apple varieties using different grafting methods in greenhouse and orchard
This study was carried out at the EÄŸirdir Horticultural Research Institute, between the years 2006 and 2007. The aim of this study was to investigate the advantages of apple nursery growing greenhouse rather than outdoor medium. Scions of Red Chief (dwarf), Braeburn (semi dwarf) and Mondial Gala (vigorous) apple varieties were grafted by the bench grafting and chip budding techniques on MM106...
December 2011
Comparative analysis of vitamin C, crude protein, elemental nitrogen and mineral content of canola greens (Brassica napus L.) and kale (Brassica oleracea var. acephala)
This study aims at promoting the consumption of canola greens (Brassica napus L.) as alternative, affordable and available dietary sources of vitamin C, minerals and protein. As one of the oldest-known forms in cabbages family, kale (Brassica oleracea var.acephala L.) greens are among the most consumed vegetable cabbages in Turkey. In this study, kale and canola greens were compared with regards...
December 2011
Decision making analysis of walnut seedling production on a small family farm in Serbia
The wide range of walnut application makes it one of the most appreciated fruit species. In 2008, Serbian government started providing subsidies for every planted walnut seedling. These incentives have already increased the interest in this type of seedlings on the market. One of the main objectives of each farm is to maximize economic results. For a family farm, there are many alternatives on how to accomplish this...
December 2011
Effect of liming on the molybdenum content in the root and leaf of tomato grown on pseudogley under controlled conditions
Pseudogley is a typical type of acid soils predominating in both Serbia and the wider region. Acid soils are not suitable for the cultivation of agricultural crops due to the hampered uptake of most nutrients as induced by increased levels of hydrogen ions. In order to make pseudogley soil suitable for crop production, pH improvement measures should be employed. The objective of this study was to use liming to...
December 2011
Studies on seed germination and in vitro shoot multiplication of Satureja khuzistanica Jamzad, an important medicinal plant
Satureja khuzistanica Jamzad is an important multipurpose medicinal plant in Iran. The essential oil of S. khuzistanica is characterized by high concentration of carvacrol (93%). The seeds of S. khuzistanica are dormant and have a very low germination under normal conditions. This is the first protocol for in vitro seed germination and plantlet regeneration of this plant. Experiments...
December 2011
Structural characters of leaf epidermis in Neolepisorus (Polypodiaceae)
The surface structure of the foliar epidermis of 5 species, 2 forms and 8 populations inNeolepisorus was investigated under light microscopes. The result shows that their stomata are distributed in the lower leaf epidermis and parallel to the veins. The types of stomata found were polocytic, axilloctytic, copolocytic, coaxilloctytic and anomotetracytic. They were different in stomatal type, stomatal index, the...
December 2011
The effect of glyphosate application on soil microbial activities in agricultural land
In recent years, intensive use of herbicides has increasingly become a matter of environmental concern partially because of the effects of these chemicals on soil microorganisms. Glyphosate [N-(phosphonomethyl) glycine] (GP) is a broad-spectrum, non-selective, post emergence herbicide that is widely used in agriculture. In this study,glyphosate effects as N, P and C nutrient sources on...
December 2011
Improvement of xanthan gum production in batch culture using stepwise acetic acid stress
In this study, the effect of acetic acid on the improvement of xanthan biosynthesis byXanthomonas campestris b82 was investigated. Various concentrations of acetic acid from 1.56 to 6.25 mM were added to the medium in the exponential and stationary phase of growth. Analytical studies revealed a considerable increase in viscosity and xanthan yield after two pulses addition of acetic acid at 24 and 26 h of...
December 2011
Physiological responses of salt stress and osmoprotection with proline in two strains of lactococci isolated from camel’s milk in Southern Algeria
The response of Lactococcus sp. strains isolated from camel milk to salt stress wascharacterized in M17 medium. We studied the growth of 20 strains in saline M17 medium, M17 medium containing various concentrations of NaCl (1.1, 1.2, 1.4, 1.6 and 1.7 M), and determined the minimal inhibitory concentration (MIC) of NaCl. Among the 20 studied strains, 2 strains presented a considerable...
December 2011
Effects of corona discharge treatment on some properties of wool fabrics
Worsted corona discharge was under operation, while the operation conditions were optimized. Processed woolen surface properties such as water absorption and staining properties were investigated. In addition, the bending length, wrinkle recovery and tensile strength retention (%) of treated samples were evaluated. Corona discharge after operation worsted water absorption property increased and the staining...
December 2011
Improved growth and quality of Dioscorea fordii Prain et Burk and Dioscorea alata plantlets using a temporary immersion system
The effects of temporary immersion system (TIS) culture on the growth and quality ofDioscorea fordii Prain et Burk and Dioscorea alata plantlets were investigated. Results indicate that TIS promoted the growth and quality of D. fordii and D. alata plantlets. Proliferation rate, shoot length, fresh weight (FW) and dry weight (DW) of shoots, and total biomass production were...
December 2011
A novel injectable and degradable calcium phosphate/calcium sulfate bone cement
The aim of this study was to obtain a composite material which not only has suitable workability but also has excellent degradability. A novel injectable and degradable bone cement consisting of a mixture of calcium phosphate (α-tricalcium phosphate; α-TCP) and calcium sulfate dihydrate (CaSO4. 2H2O; CSD) powders as the solid phase and an aqueous solution containing 2.5 weight percent of Na2HPO4 as the...
December 2011
Prevalence, perceived benefits and effectiveness of herbal medicine in the management of symptoms of opportunistic fungal infections in HIV/AIDS patients in the Eastern Cape, South Africa
It has been reported that about 75% of HIV-infected patients use remedies from complementary or African traditional medicine in South Africa. The multiplicity of these medicines led not only to potentially severe side effects, but also to clinically significant interactions with antiretrovirals (ARVs) and other medicines. The aim of this study was to evaluate the prevalence, perceived benefit and...
December 2011
A novel protein expression system-PichiaPinkâ„¢- and a protocol for fast and efficient recombinant protein expression
Pichia pastoris is a eukaryote and has many of the advantages of higher eukaryotic expression systems, such as protein processing, protein folding, and the availability of posttranslational modifications. It is as easy to manipulate as Escherichia coli orSaccharomyces cerevisiae. However, some serious and unavoidable problems occur with the Pichia pastoris system that is difficult to overcome, such...
December 2011
Aerococcus sp. with an antimycobacterial effect
Tuberculosis is an infectious disease that causes fatality every year because of drugs resistance of the Mycobacterium complex; this is why the development of new antibiotics becomes an urgent need. To reach that target, a main approach was led; consisting on screening of active substances producing microorganism. In this study, we reported data on a strain that was isolated from different areas of Fez...
December 2011
β-Cyclodextrin-curcumin complex inhibit telomerase gene expression in T47-D breast cancer cell line
Today, attempt to the preparation of stable drug with high drug delivery efficiency is inevitable. Curcumin (diferuloylmethane), with hydrophobic structure obtained from the herb of Curcuma longa, have various applications in cancer therapy. But, its low water solubility and bioavailability is possible for poor drug delivery of curcumin. In this study,we prepared β-cyclodextrin-curcumin complex to...
December 2011
Life table parameters of the dubas bug, Ommatissus lybicus (Hem: Tropiduchidae) at three constant temperatures
The dubas bug, Ommatissus lybicus Bergevin (Hemiptera: Tropiduchidae) is one of the major pests of date palm in Bam region, Iran. In this study, life table parameters of O. lybicus were studied at 25, 30 and 35°C. The experiments were conducted in a leaf cage at 60 ± 5% RH and a photoperiod of 16:8 (L: D) h. The survival rate ( lx ) of individuals developed to adults from...
December 2011
One-stop polymerase chain reaction (PCR): An improved PCR method with speedy operation and comparable efficiency
The widely used polymerase chain reaction (PCR) protocol requires several post-cycling steps to visualize amplicons, decelerating PCR sample processing and result calling. “One-stop PCR” was developed by including both the loading buffer and nontoxic staining dye within a single PCR tube, allowing direct loading and simultaneous staining of amplicons for visualization. The efficiency and sensitivity of...
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