African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: 22 January, 2014; 13(4)

January 2014

Diversity, nutritional composition and medicinal potential of Indian mushrooms: A review

Mushrooms are the higher fungi which have long been used for food and medicinal purposes. They have rich nutritional value with high protein content (up to 44.93%), vitamins, minerals, fibers, trace elements and low calories and lack cholesterol. There are 14,000 known species of mushrooms of which 2,000 are safe for human consumption and about 650 of these possess medicinal properties. Among the total known mushrooms,...

Author(s): Hrudayanath Thatoi and Sameer Kumar Singdevsachan

January 2014

Nanosilver: Potent antimicrobial agent and its biosynthesis

Silver ions and its salts are well known for their potent antimicrobial agent. These days, the nanosilver are widely used in a growing number of applications ranging from home disinfectants and medical devices to water purifier due to properties of silver at the nano level. Nanosilver has a large ratio of surface which dramatically increases the potential for silver ions to be accessed anywhere in body where larger...

Author(s): Vikas Sarsar, Krishan K. Selwal and Manjit K. Selwal

January 2014

Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle

The objective of this study was to determine the allele and genotype frequencies of genetic variants in five milk protein genes and estimate the effect of these variants on milk yield in Sahiwal cattle. Genotypes of five milk protein genes (alpha s1 casein, beta casein, kappa casein, alpha lactalbumin and beta lactoglobulin) were detected using SNaPshot genotyping method. All the five milk protein genes studied...

Author(s): Shahlla N Mir, Obaid Ullah and Riazuddin Sheikh

January 2014

Selection and optimization of extracellular lipase production using agro-industrial waste

The aim of this study was to isolate and select lipase-producing microorganisms originated from different substrates, as well as to optimize the production of microbial lipase by submerged fermentation under different nutrient conditions. Of the 40 microorganisms isolated, 39 showed a halo around the colonies and 4 were selected (Burkholderia, Bacillus sp., Penicillium lanosum and Corynebac glutamyl), where strains...

Author(s): Pabline Rafaella Mello Bueno, Tatianne Ferreira de Oliveira, Márcio Caliari, Gabriel Luis Castiglioni and Manoel Soares Soares Júnior

January 2014

New fruit and seed disorders in Papaya (Carica papaya L.) in India

Systematic field screening was conducted in 14 important papaya germplasm lines to observe the severity of bumpy fruit incidence. The disease severity, fruit yield and economic losses due to physiological disorders were observed. Pune Selection-3 was most sensitive (64.6%) for the disorder while Pusa Selection Red was most tolerant (4.2%). The maximum yield was obtained in Pune Selection-3 (62 kg/plant) while lowest...

Author(s): P. L. Saran, R. Choudhary, I. S. Solanki and P. R. Kumar

January 2014

Development of specific primers for the detection of HVA1 from barley in transgenic durum wheat by polymerase chain reaction (PCR) technology

Genetic transformation is a widely employed tool in both basic research and commercial plant breeding programs. Its application requires that transgenes be stably integrated and expressed in the plant genome. When transgenic plants are developed, it is essential to determine which plants contain the transgene. Detection methods are usually based on amplification of the target transgene. This paper describes a...

Author(s): Marouane Melloul, Driss Iraqi,  Sripada M Udupa, My Abdelaziz El Alaoui, Sanaa Amine Alaoui, Mohammed Ibriz and Elmostafa Elfahime

January 2014

Age-dependent prevalence of Loa loa amicrofilaremia and microfilaremia status as defined by two markers: microfilaria and specific IgG4

Loiasis infection is characterised by long term stability in infection status. The bases of such stability are not well known. As preliminary step toward verification of possible genetic involvement in this stability, a survey in a homogeneous population (n = 106) of a village from an endemic zone of Gabon was undertaken. The distribution of Loa loa microfilaremia according to age revealed a significant...

Author(s): Djikeussi, E. D., and Akue, J. P.

January 2014

Phytochemical and antimicrobial study on the leaf extracts of Erythrophleum africanum (Caesalpiniaceae)

The leaf of Erythrophleum africanum was exhaustively extracted with ethanol using cold maceration techniques. This was subsequently partitioned with petroleum ether, chloroform, ethylacetate and n-butanol. The agar diffusion method was used to determine the antimicrobial activity against the following micro-organisms eithicillin resistant Staphylococus, Staphylococcus aureaus, Streptococcus feacalis, Escherichia coli,...

Author(s): M. Mohammed, A. M. Musa, M. A. Garba, A. A. Adeiza and U. A. Hanwa

January 2014

Seasonal variation in phytochemicals and antioxidant activities in different tissues of various Broccoli cultivars

Florets, leaves, and stems of twelve commercial broccoli cultivars grown in the spring and fall seasons at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Suwon, South Korea were evaluated for glucosinolates, vitamin C, total phenol, and total flavonoid contents and antioxidant activity. The levels of all phytochemicals and antioxidant activity were...

Author(s): Shiva Ram Bhandari and Jung-Ho Kwak

January 2014

Antibacterial activity of honey and medicinal plant extracts against Gram negative microorganisms

There has been a steady rise in antibiotic resistance of bacteria and this urgently calls for the discovery of alternative therapeutic agents. Honey possesses therapeutic potentials which includes antimicrobial activity. Although the antimicrobial activity of honey has been effectively established against an extensive spectrum of microorganisms, it differs depending on the type of honey. To date, not much extensive...

Author(s): Ramalivhana JN, Obi CL, Samie A, Iweriebor BC, Uaboi-Egbenni P, Idiaghe J.E. and Momba M. N. B

January 2014

Establishment of a heterologous immunoassay for detecting enrofloxacin residues in poultry muscles

A heterologous indirect competitive enzyme linked immuno sorbent assay (icELISA) has been developed for the determination of enrofloxacin (ENR) residues in poultry. For this purpose, carbodiimide active ester method was employed to synthesize the artificial antigen of ENR- bovine serum albumin (BSA) while mixed-anhydride technique was used to synthesize the coating antigen of ENR- ovalbumin (OVA), to pursue the...

Author(s): Jin-qing Jiang