African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 25 July 2011; 10(38)

July 2011

Molecular diversity in Indian isolates of Fusarium oxysporum f.sp. lentis inciting wilt disease in lentil (Lens culinaris Medik)

For integrated management of wilt disease, foolproof knowledge about the genetic diversity among the prevalent isolates is very important. One hundred (100) isolates of Fusarium oxysporum f. sp. lentis, causing agent of vascular wilt in lentil were collected from different agro-climatic regions of India. Finally, fifteen distinct F. oxysporum f....

Author(s): S. Datta, R. G. Choudhary, Md Shamim, R. K. Singh and Vishwa Dhar

July 2011

Distribution of ABO and Rh-Hr blood group antigens, alleles and haplotypes in the mountain region of Ajara (Georgia)

ABO and Rh-Hr blood group antigens represent a genetically stably determined trait with many-sided biological and clinical significance. The indigenous Ajarian population (105 subjects) was investigated for ABO Rh-Hr red cell blood group antigens. Using immunoserologic methods, seven blood group antigens (A, B, C, c, D, E and e) were studied. The obtained results were statistically processed. 44 ± 4.9%...

Author(s): M. Nagervadze, A. Diasamidze, L. Akhvlediani, G. Dumbadze, R. Khukhunaishvili, M. Koridze and S. Tckvitinidze

July 2011

Pravastatin enchances the osteogenic process to the glucocorticoid-induced osteonecrosis of the femoral head

Glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH) is a disabling bone disease and it affects a lot of patients in the world widely. To investigate the effect of pravastatin on the early stage of GC-induced ONFH, white rabbits were injected twice with lipopolysaccharide (LPS) at 10 ug/kg each and three times with methyprednisolone (MPS) at 20 mg/kg each to the constructedONFH model....

Author(s): Min Hu, Hong-bin Zhao, Jin-fang Meng, Zhi-peng Yu, Hong-hui Xu, Jian-nan Fan, De-jun Luo, Chuan-yun Qian,Wei Wei and Wei Tang

July 2011

Analysis of genetic diversity in Arrhenatherum elatius Germplasm using inter-simple sequence repeat (ISSR) markers

The genetic diversity of 19 Arrhenatherum elatius accessions was analyzed using 100 inter-simple sequence repeat (ISSR) primers, out of which 11 generated distinct amplification products. Out of the 152 total bands detected, 107 were polymorphic. The percentage of polymorphic bands (PPB) was 68.9% with an average of 9.73 polymorphic bands per primer. The ISSR-based genetic similarity (GS) coefficients among...

Author(s): Lin Meng, Hong Xin Yang, Pei Chun Mao, Hong Wen Gao and Fu Ding Sun

July 2011

Effects of harvesting time on some yield and quality traits of different maturing potato cultivars

The effect of harvesting time was investigated on yield and quality traits for spring season production in different maturing potato (Solanum tuberosum L.) cultivars under agro-climatic conditions of southern of Turkey in 2006 and 2007. The experimental layout was a randomized split plot with three replications, including six potato cultivars as main plots and four harvesting times as subplots. The cultivars tested...

Author(s): Tahsin SöÄŸüt and Ferhat Öztürk

July 2011

Physiological and molecular characterization of in vitro cultures of an endemic medicinal herb, Chlorophytum borivilianum, under abiotic stress

This investigation was carried out to evaluate the effects of abiotic stress on the in vitro cultures of Chlorophytum borivilianum. Regenerated plantlets were re-inoculated on media containing different concentrations of sodium chloride (0, 34.2, 68.4, 136.8 and 171.0 µM) and mannitol (0, 10.6, 21.3, 42.7 and 53.4 µM), and thus subjected to in vitro salinity and drought stress. Both...

Author(s): Mousumi Debnath, Mukeshwar Pandey and Surendra K. Chikara

July 2011

Lowering virus attack with improved yield and fiber quality in different cotton genotypes by early sown cotton (Gossypium hirsutum L.)

A three year study with the objective of exploring the possible role of different sowing dates and cotton genotypes on seed cotton yield, fiber quality and virus attack was conducted at the Cotton Research Station (CRS), Multan, Pakistan during three consecutive years (2006, 2007 and 2008). Two cotton genotypes namely: MNH-6070 and CIM-496 were sown on five different sowing dates (15th April, 1st May, 15th May, 1st June...

Author(s): Muhammad Iqbal, Saghir Ahmad, Taj Muhammad, Mubshar Hussain, Abid Mehmood, Abdul Jabbar, Wajad Nazir, Hammad Hussnain and Nazim Hussain

July 2011

Genotypic variation in growth and physiological responses of common bean (Phaseolus vulgaris L.) seedlings to flooding

Tolerance to flooding stress in root regions of some common bean genotypes (Beyaz Fasulye, Boncuk Sırık, Kökez, Oturak and Sırık) was investigated in terms of morphological and physiological. Plants were grown in a plant growth chamber at 26/18°C (day/night) temperature with RH 70% and 450 m-2 s-1 light intensity. Seedlings were exposed to flooding stress for 3 days when the plants had...

Author(s): Gozde Celik and Ece Turhan

July 2011

De novo structural modeling and computational sequence analysis of a bacteriocin protein isolated from Rhizobium leguminosarum bv. viciae strain LC-31

Bacteriocins produced by different groups of bacteria are ribosomally synthesized peptides or proteins with antimicrobial and specific antagonistic bacterial interaction activity. Rhizobium leguminosarum is a Gram-negative soil bacterium which plays an important role in nitrogen fixation in leguminose plants. Bacteriocins produced by different strains of R. leguminosarum are known to impart...

Author(s): Azeem Mehmood Butt, Ishaque Badshah Khan#, Farhan Haq#, Yigang Tong

July 2011

Growth, ion content and photosynthetic responses of two Elytrigia Desv. species seedlings to salinity stress

Salinity is among the major abiotic stresses limiting crop production in the world.Elytrigia species, the wild relatives of wheat, are extensively used as genetic resources in wheat breeding to improve its salt tolerance. The objective of this study was to examine the responses to different NaCl treatments (0, 65, 100, 135 and 170 mM) of two Elytrigia species (Elytrigia intermedia (Host.)...

Author(s): Wang Zan, Zhang Geng, Wang Xue-min and Gao Hong-wen

July 2011

Effect of fertilizer in controlling weeds under intercropping of pearl millet and red bean in Sistan region, Iran

This experiment was conducted at the Agriculture Research Center of Zabol University during 2007 cropping season. The experimental design was split plot, using randomized complete block design with three replications. The factors included were, main factors: unfertilized (F1), 100% fertilizer (F2), 100% manure (F3), 50% fertilizer + 50% manure (F4) and five sub factors: sole crop of millet (I1), 75% millet + 25% bean...

Author(s): Issa Piri, Farshad Abrahimpour, Abolfazl Tavassoli, Ebrahim Amiri and Fatemeh Rastegaripour

July 2011

Effects of different fruit juices used as carbon source on cucumber seedling under in-vitro cultures

In this study, the effect of various commercial fruit juices (used as plant carbon source) was assessed on cucumber (Cucumis sativus L.) cv. Liza at seedling stage under aseptic conditions. Seeds were germinated on ½MS medium (within 2-days) under dark conditions. They were sub-cultured on MS0 (MS basal salts) medium and its derivatives [MS, supplemented with fruit juices in place of sucrose...

Author(s): Ikram-ul-Haq, Zara Arshad, Taseer, Ghulam Mustafa, Mukesh, Muhammad Umar Dahot, Sajid Ali and Munazza

July 2011

Assessing hydroaeroponic culture for the tripartite symbiosis of mungbean (Vigna radiata L.) with arbuscular mycorrhizal fungi and rhizobia

Mungbean (Vigna radiata L.) has the potential to establish symbiosis with rhizobia that fix atmospheric dinitrogen (N2), and arbuscular mycorrhizal fungi (AMF) that improve the uptake of low mobile soil nutrients such as phosphorus. Both rhizobial and mycorrhizal symbioses can benefit plants synergistically. The tripartite symbiosis of mungbean with rhizobia and AMF was assessed in hydroaeroponic culture under...

Author(s): Fatma Tajini, Porntip Suriyakup, Jan Jansa and Jean-Jacques Drevon

July 2011

Phytoplankton community characteristics of the icebound season and its relationship to the environmental variables in the Zhalong Wetland, China

The taxonomic structure and spatial variability of phytoplankton abundance in the icebound season was obtained from the Zhalong Wetland. A total of 109 taxa were identified in all samples, 92 taxa occurring in at least two samples or the percentages over 1% in at least one sample were utilized in further study. The algal population of the Zhalong Wetland was extremely scarce under the ice-cover condition, with a mean...

Author(s): LI Jing, QI Peishi, MA Yun and ZHOU Hao

July 2011

Batch studies on the removal of Ni (II) from aqueous solution by Azolla filiculoides

There are many plants which have the ability to accumulate large amounts of heavy metals and one of them is the aquatic fern, Azolla filiculoides. Toxic metals constitute a serious health risk because they accumulate in soils, water and organisms. One of the methods for removing these pollutants from water and soil is the use of plants. The aim of this study was to test the ability of A. filiculoides to...

Author(s): Salman Ahmady-Asbchin, Mehdi Mohammadi, Alimohamad Bahrami, Ali Louei monfared and Naser Jafari

July 2011

Cementation in a matrix of loose sandy soil using biological treatment method

Man-made materials varying from cement-based to chemical-based have been injected into soils to improve their engineering properties (shear strength, compressibility, permeability, bearing capacity etc.). Soil type in general plays important role in determination of treatment material and method. Materials used for soil treatment might have side effects in terms of air pollution, soil or water contamination etc. during...

Author(s): Ayhan Gurbuz, Yasin Dursun Sari, Zehra Nur Yuksekdag and Berat Cinar

July 2011

Physiological responses of Vallisneria spiraslis L. induced by different hydraulic conditions when exposed to copper and nitrogen

A two-flume experiment with submerged plant Vallisneria spiraslis L. was conducted to investigate the effects of different hydraulic conditions on physiological responses when exposed to water polluted with copper (Cu) and nitrogen (N). Plants were divided into two groups and grown for 120 h in hydrodynamic and hydrostatic flumes, respectively, using the same nutrient solution with fixed concentrations of 0.64...

Author(s): Peifang Wang, Chao Wang, Ping Ouyang, Jin Qian and Ruijuan Shi

July 2011

The survival successes of mycorrhizal and non-mycorrhizal Cotoneaster franchetti Bois. under different climate and various growing media

In the detailed planting applications of landscape architecture, the negativities created by insufficiencies in ecological conditions and care interferences create effects that increase economical losses. On the other hand, the drought in summer is an important problem. As a result, the plant development decreases, the nutrition relations are affected negatively, plants remain weak and they can not give the desired...

Author(s): Mustafa Var, Muberra Pulatkan and Emrah Yalcinalp

July 2011

Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus

The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified...

Author(s): Mahdi Shahriarinour, Mohd Noor Abdul Wahab, Rosfarizan Mohamad, Shuhaimi Mustafa and Arbakariya B. Ariff

July 2011

Identifiability of Baranyi model and comparison with empirical models in predicting effect of essential oils on growth of Salmonella typhimurium in rainbow trout stored under aerobic, modified atmosphere and vacuum packed conditions

The structural identifiability properties of the Baranyi model were analyzed in fitting the effect of oregano and thyme essential oils on growth of Salmonella typhimuriumin rainbow trout stored under aerobic (AP), modified atmosphere (MAP) and vacuum packed (VP) conditions. Although, formally proven to be structurally identifiable using the Taylor-series approach, the Baranyi model was not practically...

Author(s): Mustafa Tahsin Yılmaz

July 2011

Effects of different autotoxins on antioxidant enzymes and chemical compounds in tea (Camellia sinensis L.) Kuntze

Continuous cropping obstacle is a serious problem in tea (Camellia sinensis L.) planting. In order to understand the mechanism of this phenomenon, SOD, POD, CAT and MDA in the roots of tea tree and chemical compounds in leaf of tea were analyzed. The results showed that the autotoxins had both inhibitory and stimulatory effects on antioxidant activities, stimulatory effects increased with time at low...

Author(s): Panrong Cao, Chunyuan Liu and Dan Li

July 2011

Phytochemical analysis of selected medicinal plants

Four medicinal plants including Ranunculus arvensis, Equisetum ravens,Carathamus lanatus and Fagonia critica were used for the study. All the plants were biologically active and were used for different types of ailments. Keeping in view their importance, this work was carried out to investigate the quantitative determination of their crude phytochemicals, vitamins and protein...

Author(s): Iqbal Hussain, Riaz Ullah, Rooh Ullah , Muhammad Khurram, Naseem Ullah, Abdul Baseer, Farhat Ali Khan, Muneeb ur Rehman Khattak, Mohammad Zahoor, Jehangir Khan and Naeem khan

July 2011

Therapeutic hypothermia reduces intestinal ischemia/reperfusion injury after cardiac arrest in rats

To investigate the effects of therapeutic hypothermia (TH) on the morphology and function of intestine after cardiac arrest and resuscitation, 45 male rats were randomly assigned into three groups: (1) normothermia group, animals underwent ventricular fibrillation (VF) and cardiopulmonary resuscitation (CPR) with the rectal temperature maintained at 36.8 ± 0.2°C until 4 h after return of spontaneous...

Author(s): Jun Zhu, Yue Fu, Jun Jiang, Jia-Kang Liang, and Zi-Tong Huang,

July 2011

Effects of recombinant human nerve growth factor on cervical cancer

Nerve growth factor (NGF) plays a crucial role in the life of the sympathetic and sensory nervous systems. However, the roles of NGF to cervical cancer remain deeply unknown. This study investigated the effect of recombinant human nerve growth factor (rhNGF) on cervical cancer. It was found that the proliferation of helacells was inhibited by rhNGF and there were some apoptotic-like cell deaths. After...

Author(s): Huaiping Shi#, Wenye Li#, Guoxia Geng, Qingwang Li, and Junjie Gao

July 2011

Effect of hypoxia-inducible factor 1-alpha (HIF-1α) on proliferation and apoptosis of adrenocorticotropic hormone (ACTH)-secreting pituitary adenoma cells

To understand whether hypoxia-inducible factor 1-alpha (HIF-1α) could protect AtT-20 cells from hypoxia induced apoptosis, we investigated the effects of HIF-1α on proliferation and apoptosis of adrenocorticotropic hormone (ACTH)-secreting pituitary adenoma cells (AtT-20 cells). AtT-20 cells were treated with various concentrations of CoCl2 to induce hypoxia....

Author(s): Sijian Pan, Bengeng Pei, Chen-Ran Zhang, Changyan Fan, Liu-Guan Bianand Qing-Fang Sun

July 2011

High-density cultivation of Lactobacillus plantarum NCU116 in an ammonium and glucose fed-batch system

Lactic acid bacteria (LAB) constitute a heterogeneous group of bacteria which are found in diverse environments such as the human body, animals and plants. In this study, we reported a simple fed-batch process for Lactobacillus plantarum NCU116 with high cell density. It was found that the optimum initial glucose concentration for this strain was 5% (w/v). To reduce the effect of acid and starvation, the...

Author(s): Tao Xiong, Xuhui Huang, Jinqing Huang, Suhua Song, Chao Feng,and Mingyong Xie,

July 2011

The effects of Saccharomyces cerevisiae on performance and nutrients digestibility in broilers fed with diet containing different levels of phosphorous

The effects of yeast culture (YC) with different levels of NPP (non phytate phosphorus) on performance, tibia and blood parameters and ileal digestibility of nutrients were investigated in broilers. A total of 720 one-day-old male broiler (Ross) chicks were divided into 48 groups and fed with 12 diets (4 groups per diet) for 42 days. The experimental design was a randomized complete block with a 3 × 4 factorial...

Author(s): Akhavan-Salamat, H., Ghasemi, H. A., Khaltabadi-Farahani, A. H. and Kazemi-Bonchenari, M.

July 2011

Erythrocyte potassium and glutathione polymorphism determination in Saanen x Malta crossbred goats

This research is aimed at determining the erythrocyte potassium and glutathione polymorphisms and also to identify the relationship among the various blood parameters in Saanen x Malta crossbred goat raised in Turkey. The allele gene frequencies of KH and KL associated with the potassium concentration were calculated as 0.94 and 0.06, respectively. The differences between the mean values of low...

Author(s): Eser Kemal Gurcan, Ozden Cobanoglu and Merve Kose

July 2011

Effects of dietary supplementation of multi-enzyme complex on the energy utilization in rooster and performance of broiler chicks

Two experiments were conducted to determine the effects of dietary supplementation of REAP® enzyme into corn-soybean diet on the energy utilization in poultry and performance of broiler chicks. In the first experiment, a total of 16 50 weeks adult roosters (ISA-Brown) were divided into 4 groups with 4 birds per replicate and the experimental diets contained the two levels of energy (2650 and 2759 kcal TMEn/kg...

Author(s): Fatemeh Shirmohammad and Morteza Mehri

July 2011

Molecular authentication of the traditional Tibetan medicinal plant, Meconopsis impedita

The root of Meconopsis impedita is one of the most widely used traditional Tibetan medicinal plants. However, it is often confused with Meconopsis racemosa whose roots may be used as a substitute. To establish a DNA polymorphism-based assay for the identification of M. impedita, the chloroplast rps16 intron region of 11 accessions representing M....

Author(s): Rong Li and Zhiling Dao