African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12198

Table of Content: 27 August, 2014; 13(35)

August 2014

Micropropagation of Caralluma stalagmifera var. longipetala: A rare succulent medicinal plant from Karnataka, India

An efficient in vitro protocol has been developed for the multiplication of shoots and conservation of a rare succulent medicinal plant Caralluma stalagmifera var. longipetala growing wildly in Karnataka State. Proliferation of multiple shoots was achieved on Murashige and Skoog’s (MS) medium supplemented with various concentrations of 6-benzyladenine (BA), Kinetin (Kn), indole acetic acid (IAA), α-...

Author(s): Ramachandra Naik M., Subbiah Karuppusamy and Y. L. Krishnamurthy

August 2014

Assessment of genetic diversity of rice (Oryza sativa) cultivars using ssr markers

A set of 36 polymorphic simple sequence repeat (SSR) primers well distributed on all the 12 rice chromo-somes have been used to assess the genetic diversity among the rice varieties. A total of 98 alleles were detected with an average of 2.78 alleles per locus across 39 genotypes.  The number of alleles varied from two to four of these SSR loci. Among the primers, RM 401, RM 20A, RM 536, RM 7575 and RM 5862...

Author(s): M. Umadevi, P. Veerabadhiran and S. Manonmani

August 2014

Effect of seasonal collection on callus Induction, proliferation and somatic embryogenesis from anther cultures of Heveabrasiliensis MuellArg

Hevea brasiliensis that is grown in Thailand and Southeast Asia is a very valuable source of natural rubber. However, few studies have been conducted to evaluate the effects of seasonal effect on plantlet regenera-tion through somatic embryogenesis from anther culture. Thus, this study discussed the effect of season on callus induction subsequent to plantlet regeneration from culturing of anther. Male flowers were...

Author(s): Wutthichai Srichuay, Soontreeya Kalawong, and Sompong Te-chato

August 2014

2,4-Dichlorophenoxyacetic acid increases reserve compounds and spectaline contents in Senna spectabilis calli

The aim of this study was to develop an in vitro culture system for Senna spectabilis and to quantify contents of storage compounds and spectaline in induced calli in relation to exogenous auxin. Explants (cotyledon, hypocotyl, epicotyl, and leaf) were cultured on MS medium containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). The soluble carbohydrate, starch, soluble protein and spectaline...

Author(s): Monaly Sado, Armando Reis Tavares and Edison Paulo Chu

August 2014

An attempt towards standardization of the production process of dawadawan botso (a fermented condiment)

The production of ‘dawadawan botso’ a local condiment produced by traditional uncontrolled fermentation, in Nigeria and other parts of Africa is usually based on experience rather than standard measurements. This work was aimed at evaluating the effect of substrate (Hibiscus sabdariffa seeds) and ash leachate on the chemical composition, amino acid profile and the taste of ‘dawadawan botso.'...

Author(s): Ibrahim, A. D., Pedavoah, M. M., Bawa Saadatu M. and Sani, A.

August 2014

Effects of different feed amounts on the growth performance of gilthead sea bream (Sparusaurata) in the Black Sea

The aim of this study was to investigate the growth performanceof gilthead sea bream (Sparusaurata) fed by different feed amounts in the Black Sea. The gilthead sea bream with an average weight of 11.24±0.04 g was fed by restricted amounts of food (Group I) and ad libitum (Group II) for 127 days. At the end of the study, the average weight and feed conversion rates of Group I and Group II were 70.58±0.79...

Author(s): Birol Baki

August 2014

Thidiazuron-induced in vitro bud organogenesis of the date palm (Phoenix dactylifera L.) CV. Hillawi

The objective of the present was to enhance the frequency of plant regeneration in date palm (Phoenix dactylifera L.) cv. Hillawi. Explants were incubated on Murashige and Skoog (MS) medium supplemented with 1 mgl-1 6-benzyladenine (BA) and different concentrations (0.1 to 2.0 mgl-1) of thidiazuran (TDZ), or free of BA and TDZ (control treatment). The results indicate that the Maximum response (66.67%) was observed on...

Author(s): Ahmed Madi Waheed Al-Mayahi

August 2014

Response of okra [Abelmoschus esculentus (L.) Moench] to water stress in the soil

Water stress effects induced by stopping watering at vegetative (S1) and flowering (S2) stages were studied on five varieties of okra [Abelmoschus esculantus Moench (L.)]. Parameters such as relative water content, membrane permeability, chlorophyll content and yield of capsules varieties were evaluated. Our results show that water deficiency reduces relative water content of okra leaves. This reduction is more...

Author(s): Rasmata NANA, Mahamadou SAWADOGO, Zoumbiessé TAMINI

August 2014

Henna wood as an adsorptive material for bentazon

In this study, the efficiency of activated carbon produced from Henna wood was studied to remove herbicide from aqueous solutions by adsorption. The parameters that affect the adsorption such as contact time, activated carbon dosage, initial concentration of adsorbate, stirring rate, temperature, and pH on bentazon adsorption were studied. The use of Henna wood as a raw material to produce activated carbon by physical...

Author(s): Brahmi Mounaouer, Ahmed Wali, Olfa Fourti and Abdennaceur Hassen

August 2014

Physico-chemical and biotic factors influencing microalgal seed culture propagation for inoculation of a large scale raceway pond

The growth of Chlorella vulgaris in open pond aquatic conditions poses serious challenges due to the interplay of both physico-chemical and biotic factors. We report here the monitoring of physico-chemical and biotic parameters affecting the propagation of C. vulgaris seed culture for inoculation of a large scale raceway pond (300 000 L capacity) in South Africa. The C. vulgarisstrain used for this purpose was isolated...

Author(s): Mutanda, T., Ranjith Kumar R. and Bux, F.

August 2014

Antimicrobial activities, toxinogenic potential and sensitivity to antibiotics of Bacillus strains isolated from Mbuja, an Hibiscus sabdariffa fermented seeds from Cameroon

This study was carried out to screen for antimicrobial activities against common pathogenic and food spoilage bacteria, yeasts and molds, toxinogenic potential and sensitivity to antibiotics of 26 Bacillus strains (11 Bacillus subtilis, 5 Bacillus megaterium, 4 Bacillus amyloliquefaciens, 4 Bacillus pumilus and 2 Bacillus thuringiensis) isolated from different samples of Hibiscus sabdariffa fermented seeds (Mbuja)....

Author(s): Bouba-Adji Mohammadou, Gwenaelle Le Blay, Carl Moses Mbofung and  Georges Barbier