African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12478

Table of Content: 29 June 2011; 10(31)

June 2011

Parentage verification and identity test of Ghezel sheep using microsatillate markers

The Ghezel sheep is a fat tail high weight Iranian breed which is raised in the North-west of Iran. To design an efficient improvement program and genetic evaluation system for this indigenous breed, accurate estimates of the population genetic parameters is per-required and all pedigrees and relationships should be correctly recorded. Otherwise, it can produce biased evaluations when pedigrees contain errors and...

Author(s): Adel Saberivand, Arash Javanmard and Marryam Safdari      

June 2011

Random amplified polymorphic DNA (RAPD) marker associated with salt tolerance during seeds germination and growth of selected Acacia senegal provenances

Seed germination and seedling growth of 20 provenances of Acacia senegal were evaluated under salinity conditions. For germination study, seeds irrigated with NaCl in five concentrations had electrical conductivities (EC) of 0 (only distilled water), 2, 4, 6 and 10 dSm-1. Final germination percentage (FG) and germination rate index (GRI) were recorded during 14 days. The result showed significant effect...

Author(s): Hussien M. Daffalla, Rami S. Habeballa, Elsadig A. Elhadi and Mutasim M. Khalafalla      

June 2011

Expression patterns of homeobox genes in different teeth of the minipig embryo

Tooth development is a multi-stage process that is regulated by complex molecular events and the minipig is a good model for tooth development research. However, the expression patterns of homeobox genes during tooth development of the minipig are still unclear. In this work, we utilized the Chinese experimental minipig as a model to examine expression patterns of homeobox genes in different teeth...

Author(s): Jirong Yin, Ying Zhang, Yuxing Bai, Bangkang Wang and Kai Yang        

June 2011

A fresh method of DNA transformation to the seeds irradiated by 60Co without the use of antibiotic selection

To find out a simpler method that can directly transfer the aim gene into plant genomes, the purple medic seeds irradiated by 60Co with 0.375 Gy were transformed by linear DNA containing a β-glucuronidase (GUS) gene (as an aim gene), a betaine aldehyde dehydrogenase (BADH) gene (as a selectable marker) and two pairs of both CaMV35S promoter and Nos terminator. Subsequently, the seeds...

Author(s): Xinglin Li, Dandan Wang, Jie Gao, Tianjian Xiao, Xiaojuan Wang, Liming Zhang, Dong Liu, Limei Li, Fuping Lu  and Wen-yuan Gao          

June 2011

Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake

Trichoderma species are commonly used as biological control agents against phytopathogenic fungi and some isolates are able to improve plant growth. In this study, the effects of three Trichoderma isolates including Trichoderma harzianumisolate T969, T. harzianum isolate T447 and Trichoderma sp. isolate T in tomato seedling vigor and their nutrient uptake via...

Author(s): Rasool Azarmi, Behzad Hajieghrari and Abolfazl Giglou      

June 2011

The influence of endogenous hormones on the formation of buds from stems of bitter melon (Momordıca charantıa L.)

Stems of bitter melon (Momordica charantia L.) cv. Dabai were used to establish in vitro cultures. The endogenous hormone concentrations (indoleacetic acid [IAA], abscisic acid [ABA], gibberellins 3 [GA3], zeatin [ZT]) of the calluses were determined by means of high pressure liquid chromatography (HPLC). The endogenous ZT was higher in the stem calluses that had formed buds, and there was a...

Author(s): Yi Tang, Ji Liu, Juan Li, Xiao-Mei Li, Bin Liu and Huan-Xiu Li      

June 2011

Herbal gardens of India: A statistical analysis report

A knowledge system of the herbal garden in India was developed and these herbal gardens’ information was statistically classified for efficient data processing, sharing and retrieving of information, which could act as a decision tool to the farmers, researchers, decision makers and policy makers in the field of medicinal plant species.    Key words: Knowledge system, herbal garden,...

Author(s): N. Srinivasa Rao and Susanta Kumar Das    

June 2011

The effects of different irrigation regimes on yield and silage quality of corn under semi-arid conditions

The aim of this study was to evaluate the effects of different quantities of drip irrigation water on the corn yield and silage quality under semi-arid conditions. The seasonal evapotranspiration (ETc) for the different irrigation regimes varied from 434 to 947 mm in 2004 and from 468 to 1003 mm in 2005 for total irrigation periods of 108 and 113 days, respectively. Combining data from both years,...

Author(s): Mehmet Simsek, Abdullah Can, Nihat Denek and Tahsin Tonkaz      

June 2011

Corn stover-enhanced cellulase production by Aspergillus niger NRRL 567

he production of extracellular cellulases by Aspergilus niger NRRL 567 on corn stover was studied in liquid state fermentation. In this study, three cellulases, exoglucanase (EXG), endoglucanase (EG) and b-glucosidase (BGL) were produced by A. niger NRRL 567. The optimal pH, temperature and incubation time for cellulases production was found to be 3.5, 30°C and 96 h, respectively....

Author(s): Muhammad ishfaq Ghori, Sibtain Ahmed, Muhammad Aslam Malana and Amer Jamil

June 2011

Assessment of serum biochemistry in West African Dwarf (WAD) does administered varying levels of medroxyl–progesterone acetate (MPA), an estrus synchronizing drug

This study was carried out to assess the health status (serum biochemistry) of West African Dwarf (WAD) does administered varying levels of modroxyl-progestrone acetate (MPA), an oestrus synchronizing drug. The experiment was laid out in a completely randomized design. Result of the experiment showed that all the does were in good health status before, during and after the drug administration. Blood serum electrolytes...

Author(s): Imasuen, J. A. and Otoikhian, C. S. O.    

June 2011

Clinical comparison of localized gingival recession coverage in root surfaces restored with giomer and intact root surfaces

This controlled clinical study was aimed to evaluate the root coverage procedures using connective tissue graft (CTG) on non-carious root surfaces in comparison with carious lesions, restored by giomer (g-CTG). Fifteen (15) patients with 30  Miller class I or II defects including 15 carious root lesion (test group) and 15 sound root surfaces (control group) were selected. The carious roots were restored...

Author(s): Reza Pourabbas, Soodabeh Kimyai, Mohammad Taghi Chitsazi and Fatemeh Nourbaksh    

June 2011

Antimicrobial and anti-inflammatory activities of the volatile oil compounds from Tropaeolum majus L. (Nasturtium)

This study was carried out to evaluate the antimicrobial and anti-inflammatory activity of some chemical compounds of the volatile oil extracted from Tropaeolum majus L. The chemical compounds extracted from the flowers and leaves of T. majus L. have been identified through color reactions and gas-chromatographic analysis combined with mass spectrometry. Quantitative testing and...

Author(s): Monica Butnariu and Cristian Bostan      

June 2011

Effect of different solvent extracted sample of Allium sativum (Linn) on bacteria and fungi

ZI, Zone of inhibition.

Author(s): Jehan Bakht, Muhammad Tayyab, Huma Ali, Amjad Islam and Mohammad Shafi      

June 2011

Homology modeling of ɣ-aminobutyrate-aminotransferase, a pyridoxal phosphate-dependent enzyme of Homo sapiens: Molecular modeling approach to rational drug design against epilepsy

É£-Aminobutyrate aminotransferase (GABA-AT) is a pyridoxal phosphate dependent homodimeric enzyme of 50-kD subunits. It is a potential drug target against epilepsy. The three-dimensional structure of GABA-AT is not experimentally known, and we thus resorted to homology modelling to build a model based on x-ray crystal structure of pig liver GABA-AT to 3.0 Å resolution. Knowledge of the three-dimensional structure...

Author(s): Hina Naz Khan, Hamid Rashid and Saima Kulsoom