August 2012
Intraspecific variation in pollen viability, germination and ultrastructure of Olea europaea L.
Variability of pollen morphology and ultrastructure of Olea europaea L. (Oleaceae) cultivars ‘Koroneiki’, ‘Mastoidis’ and ‘Kalamata’ was studied with scanning electron microscopy to identify genotype-distinguishing characters that could be employed for morphological cultivar discrimination. Pollen viability and germination was also measured for the three...
August 2012
A simple and rapid lysis method for preparation of genomic DNA from Gram-negative bacteria
A very efficient, simple and economic lysis method for rapid preparation of genomic DNA from Gram-negative bacteria was developed. This method includes a novel step lysis by treatment with carvacrol followed by simple ethanol precipitation. The procedure was realised without using of detergents or enzymes. Moreover, the resultant genomic DNA was in good quantity and quality and can be used...
August 2012
Functional analysis of a gene encoding homoserine kinase from rice
Homoserine kinase (HSK) is an enzyme that catalyzes the common pathway in threonine and methionine biosynthesis in plants. The genes encoding HSK have been reported in many bacteria and some plants including Arabidopsis. Our group recently reported a gene for threonine synthase (TS) from rice. In this study, we functionally characterized a gene encoding HSK from rice (OsHSK). Analysis of a cDNA sequence and...
August 2012
Cloning and analysis of plant fatty acid desaturase 7 gene promoter from Brassica napus
n order to investigate the regulation mode of Brassica napus FAD7 gene in response of thermal stress, we measured the protein levels of BnFAD7 in plant at low and high temperature, and then analyzed promoter activity of 5’-flanking regions of BnFAD7 by transient gene expression in B. napus protoplasts at different temperatures. Our studies indicated...
August 2012
Differential responses of two rubber tree clones to chilling stress
Chilling stress is one of the most important environmental factors that limit the growth, distribution and yield of rubber tree in China. The effects of chilling stress on the grated plants of two rubber trees clones, GT1 and Wenchang217, were studied by physiological methods in controlled light chamber in order to explore the physiological mechanism of cold tolerance in rubber tree. Our results show a...
August 2012
Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac
The system of somatic embryogenesis of Amorphophallus konjac had been built through unpollinated ovaries. The embryogenic calli were induced on Murashige and Skoog (MS) basal medium supplemented with 9.0 μM 6- benzylaminopurine (BA), 0.4 μM 2,4- dichlorophenoxyacetic acid (D), 1.0 μM naphthaleneacetic acid (NAA), and the induction rate was 34.0%. The differentiation...
August 2012
In vitro effects of salicylic acid, calcium and copper ions on growth and sporulation of Ganoderma boninense
The in vitro effects of single and combined application of calcium ion (Ca2+), copper ion (Cu2+) and salicylic acid (SA) were evaluated on growth and sporulation ofGanoderma boninense. In poison medium test, T7-(Ca+Cu+SA) showed effective control of G. boninense in-vitro with EC50 and EC90 values of 1500+150+150 ppm and 2000+200+200 ppm, respectively. ...
August 2012
Cost effective pilot scale production of biofertilizer using Rhizobium and Azotobacter
We standardized the protocol for pilot scale production of Rhizobium and Azotobacterbiofertilizer technology using region specific and environmental stress compatible strains isolated from various agro-climatic regions of Odisha, India. The cost benefit of biofertilizer production through a cottage industry is also presented. With an investment of $5000 as fixed cost, recurring expenses...
August 2012
Comparative studies of ginger (Zingiber officinale) and black pepper (Piper guinenses) extracts at different concentrations on the microbial quality of soymilk and kunuzaki
Extracts of two spices namely ginger (Zingiber officinale) and black pepper (Piper guinenses) were prepared in 0.4, 1.2, 2.4 and 3.6% concentrations. Soymilk and kunuzaki, were treated, respectively with the different concentrations and stored at ambient temperature for 5 days. The microbial load and identification were determined every day of storage until samples were adjudged spoiled. On the first day,...
August 2012
Anti-hyperlipidemic and biochemical effect of extract of Tulbaghia violacea rhizomes on high cholesterol diet fed rats
Anti-hyperlipidemic and biochemical effect of methanolic extract of Tulbaghia violacearhizomes were investigated in cholesterol rich diet-induced hypercholesterolemia Wistar albino rats. Hypercholesterolemia was induced in rats by feeding with 2% cholesterol rich diet (high cholesterol diet, HCD) for four weeks. Some biochemical parameters and histology of the liver were assessed following 28 days...
August 2012
In vitro micro-propagation of Longiflorum-Asiatic (LA) hybrids lily (Lilium) cultivar ‘eyeliner’
Bulblets propagation by tissue culture was one of the key techniques in the production of lily (Lilium) bulbs. Therefore, in vitro micro-propagation of lily bulblets was studied in detail in this paper. L A hybrids lily cultivar ‘eyeliner’ was selected as the materials. By using the method of orthogonal design, the following were concluded from the research: the optimum treatment and...
August 2012
Development of an indirect method of microalgal lipid quantification using a lysochrome dye, Nile red
Earlier studies showed that the lipophilic dye, Nile red (9-diethylamino-5H-benzo[α]phenoxazine-5-one) can be used to measure the lipid content of microalgae by cellular in vivo fluorescence. It was observed that a higher amount of lipid present in lipid droplets of microalgal cells would result in higher degree of emitted fluorescent light. In this present study, the feasibility of using Nile...
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