African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Table of Content: 31 May 2010; 9(22)

May 2010

Genetic diversity in pigeonpea [Cajanus cajan (L.) Millsp.] Landraces as revealed by simple sequence repeat markers

  Genetic relationships among 88 pigeonpea accessions from a presumed centre of origin and diversity, India and a presumed secondary centre of diversity in East Africa were evaluated using six microsatellite markers. Forty-seven (47) alleles were detected in the populations studied, with a mean of eight alleles per locus. Populations were defined by region (India and East Africa) and sub-populations by country...

Author(s): Sarah Songok, Morag Ferguson, Anne W. Muigai and Said Silim

May 2010

Cloning and molecular characterization of glyceraldehyde-3-phosphate dehydrogenase gene from thermotolerant mushroom, Lentinus polychrous

 This study describes the cloning and expression analysis of the heat shock protein gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), in thermotolerant mushroom Lentinus polychrous, one of the most widely cultivated commercial edible mushroom in Thailand. The complete GAPDH coding sequence contained 1,011 bp, encoding for a polypeptide of 337 amino acid residues with the calculated molecular mass of 36.2...

Author(s): Pornthap Thanonkeo, Rattanagon Monkeang, Weerasak Saksirirat, Sudarat Thanonkeo and Koichi Akiyama

May 2010

Proximate composition and mineral profile of eight different unstudied date (Phoenix dactylifera L.) varieties from Pakistan

  With the aim of extending the knowledge on chemical composition of dates (Phoenix dactylifera L.), eight different sun dried date varieties; (1) Daki, (2) Aseel, (3) Coconut, (4) Khuzravi, (5) Halavi, (6) Zahidi, (7) Deglet Noor and (8) Barkavi were examined to determine their proximate composition and mineral profile. All the date varieties were found to be rich in proteins, fiber, carbohydrates and net...

Author(s): Muhammad Salman Jamil, Raziya Nadeem, Muhammad Asif Hanif, Muhammad Asif Ali, and Kalsoom Akhtar

May 2010

The influence of aqueous leaf and stem extracts of Adenia lobata (Jacq) on the flowering and fruiting of okra (Abelmischus esculenta) and groundnut (Arachis hypogea)

  The influence of the aqueous leaf and stem extracts of Adenia lobata (Jacq) on the flowering and fruiting of okra (Abelmischus esculenta) and groundnut (Arachis hypogea) was investigated. A. lobata extract prepared in the following dilutions: 1:1, 1:2.5 and 1:5 (v/v) from the original extracts and distilled water, which served as control were used for treatments....

Author(s): G. G. E Osuagwu and I. C. Ibeabuchi

May 2010

Enzyme activity in bioregulator-treated tomato (Solanum lycopersicon) genotypes

  The challenges of using exogenous plant bioregulators to manipulate plant responses by researchers and producers are increasing rapidly. In previous studies, we have demonstrated that 100 mg/l concentration of the bioregulators; indole-acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) is suitable for tomato growth in the field. In this work, spectrophotometric analysis was...

Author(s): Charles O. Olaiya

May 2010

Improved preservation effects of litchi fruit by combining chitosan coating with ascorbic acid treatment during postharvest storage

  Pericarp browning and aril decay of litchi fruits shorten post-harvest storage and thus reduce market value. Effects of chitosan coating and ascorbic acid (AsA) on litchi fruits storage were investigated in this paper. Based on the fact that AsA increases anti-oxidation capacity and chitosan inhibits dehydration and microbial attack, the novel strategies of combining chitosan with AsA treatment were developed...

Author(s): Dequan Sun, Guobin Liang, Jianghui Xie, Xintao Lei and Yiwei Mo

May 2010

Studies on Meloidogyne javanica infestation on roma tomato (Lycopersicon esculentum Mill) under different soil amendments

 The effect of different soil amendments on infestation of Meloidogyne javanica on roma tomato (Lycopersicon esculentum) was investigated at the Federal College of Agriculture, Ishiagu, Nigeria using pot experiment. Completely randomized design was used for experiment and data collected were subjected to analysis of variance (ANOVA). All significant differences observed were separated using...

Author(s): S. I. Ogwulumba, K. I. Ugwuoke and R. O. Ogbuji

May 2010

Biochemical/physiological characterization and evaluation of in vitro salt tolerance in cucumber

  The difference in biochemical and physiological parameters of selected tolerant, medium tolerant and sensitive genotypes of cucumber (Cucumis sativus L.) derived from in vitro screening was investigated in order to put forward the relative tolerance or sensitivity of the genotypes and to identify parameters that can be used as index for in vitro evaluation of salt tolerance in...

Author(s): Ahmed Abbas Malik, Wei-Guan Li, Li-Na Lou, Jia-Hua Weng and Jin-Feng Chen

May 2010

Physico-chemical characterisation of some ground water supply in a school environment in Ilorin, Nigeria

  The water quality assessment of some ground water supply to a school in Ilorin City was carried out over a year. The concentration of nitrates in the samples were determined using a UV - visible spectrophotometer. The wells located within the student hostels were found to be high in nitrate with concentrations ranging from 7.11 - 8.54 mg/l. This is close to the World Health Organization (WHO) limit of 10 mg/l...

Author(s): O. A. A. Eletta, A. G. Adeniyi and A. F. Dolapo

May 2010

Biodegradation of a refinery effluent treated with organic fertilizer by modified strains of Bacillus cereus and Pseudomonas aeruginosa

  Bacillus cereus and Pseudomonas aeruginosa, that possess increased potential to degrade petroleum products exposed to UV-irradiation for 30 min followed by nitrous acid treatment, were employed in the biodegradation of effluent of a Refining and Petrochemical Company treated with NPK 15-15-15 organic fertilizer. While the untreated effluent achieved 89.25 and 77.63% degradation of oil and...

Author(s): Idise, O. E, Ameh, J.B., Yakubu, S.E. and Okuofu, C.A.

May 2010

Modification of Bacillus cereus and Pseudomonas aeruginosa isolated from a petroleum refining effluent for increased petroleum product degradation

  Bacillus cereus and Pseudomonas aeruginosa isolated from Kaduna refining and petrochemical company (KRPC) were subjected to ultra-violet (UV)-irradiation for 30 min followed by nitrous acid treatment and re-irradiated with UV light for 30 min using standard methods and their petroleum degradation abilities were compared with parent strains. Prior to modification, B. cereus and P....

Author(s): Idise O. E., Ameh J. B., Yakubu S. E. and Okuofu C. A.

May 2010

Preliminary study on metabolic regulation and control of L-valine fermentation in a newly screened L-valine producing Brevibacterium flavum strain

 A L-valine hyper-producer Brevibacterium flavum XQ-6 (Leul, Ilel, AHVr, α-ABhr, 2-TAhr) was obtained, which could be resistant to high concentration of α-amino-butyric acid (α-AB) and 2-thiazolealanaine (2-TA). The metabolic network of XQ-6 was regulated with the addition of amino acids, organic acids, vitamins, bases and other organic things. The carbon flux was directed to L-valine by...

Author(s): Liu Huanmin and Zhang Weiguo

May 2010

Myeloperoxidase enzyme levels and oxidative stress in bipolar disorders

  Oxidative stress and generalized inflammatory state are features of bipolar disorders (BD). The objective of this study was to compare the levels of products of inflammatory reaction and oxidative stress markers in patients with bipolar disorders and attention deficit/hyperactivity disorder (ADHD) and to determine the relationship between oxidative stress and inflammation in bipolar disorders. ADHD+BD (n =...

Author(s): S. Nur Aksoy, Eyup Ilker Saygılı, Ferıdun Bulbul, Aynur Bahar, Haluk Savas Osman Vırıt, Abdurrahman Altındag, Aysen Bayram and Mehmet Tarakçıoğlu

May 2010

Nutritional alternatives on the haematological and biochemical changes associated with experimental trypanosomiasis in rats

  The influence of improved nutrition on the development of pathophysiological effect of trypanosome in rats fed with two different levels of protein was investigated. The intensities of parasitaemia indicated that there was a tendency for animals receiving the high protein to sustain more parasite numbers than those receiving low protein diets. The infected animals on the two diets showed similar degree of...

Author(s): T. O. S. Adewoga, A. Sebiomo, O. O. Shogunle and R. E. Antai

May 2010

Sterol composition of caper (Capparis spinosa) seeds

 Caper is a perennial shrub of the Mediterranean Basin. The most important economical species is Capparis spinosa. Sterols of C. spinosa seed oil isolated from seven Tunisian stands were identified and quantified. C. spinosa contained high levels of phytosterols (2240.4 mg/kg of total extracted lipids), of which β-sitosterol, with 1390 mg/kg, was the most abundant (57.53%)....

Author(s): Nizar Tlili , Nizar Nasri, Ezzeddine Saadaoui, Abdelhamid Khaldi and Saida Triki

May 2010

Clinical investigation of CT-guided ozone-blowing and fumigation therapy for the chronic refractory cutaneous sinus and ulcer

  The purpose of this article is to investigate the clinical effect of CT-guided ozone-blowing and fumigation in the treatment of chronic refractory cutaneous sinus and ulcer. Twenty-six cases of chronic refractory sinuses and ulcers caused by different diseases were retrospectively analyzed in our study. After sterilization, all patients were scanned by computed tomography and the sinuses and ulcers...

Author(s):   Meng-Qi Wei, Jian-Shou Zhou, Hai-Tao Liu, Yuan Yuan, Jun-Qing Xu, Yong Yang, Jin-Song Zhang, Yong Peng and Yi Huan