May 2012
Arabidopsis rad23-4 gene is required for pollen development under UV-B light
Nucleotide excision repair (NER) is a highly conserved DNA repair pathway for correcting DNA lesions that cause distortion of the double helical structure. The protein heterodimer Rad23 is involved in recognition and binding to such lesions. Here, we showed that rad23-4 (AT5g38470) was expressed in the roots, mature leaves, floral buds and developing siliques. The collapsed pollen grains were observed...
May 2012
A new caerin-like antibacterial peptide from the venom gland of the Iranian scorpion Mesobuthus eupeus: cDNA amplification and sequence analysis
Scorpion venom consists of different types of peptides and proteins which are encoded by individual genes. A full length cDNA consisting of 238 base pair nucleotides and encoding 74 amino acids peptide was isolated from the venom gland of the Iranian scorpion Mesobuthus eupeus (Buthidae family). This peptide named M. eupeus caerin-like antimicrobial peptide (Me-CLAP) belonging to the group of...
May 2012
Enhanced in vitro multiple shoot induction in elite Pakistani guava cultivars for efficient clonal plant multiplication
Elite guava (Psidium guajava L.) strains of cv. Safeda were explored in vitro for multiple shoot induction. Shoot induction was enhanced up to 83% with 3.5 to 4.25 shoots per single node cutting and shoot tip explants, respectively, using higher levels of benzyl amino purine (BAP) in Murashige and Skoog (MS) medium. Enhancing sucrose supplement to 45 gl-1 improved plant growth and...
May 2012
Morphological and anatomical response of Acacia ehrenbergiana Hayne and Acacia tortilis (Forssk) Haynes subspp. raddiana seedlings to induced water stress
The response of Acacia ehrenbergiana Hayne and Acacia tortilis (Forssk) Haynes subspp. raddiana seedlings to 100, 50 and 25% field capacity (FC)watering regimes was studied to determine their morphological and anatomical behaviour. Both species responded morphologically as well as anatomically to water stress. Water stress caused significant (P=0.05) decrease in relative water...
May 2012
Detection of Hevea brasilensis clones yield potential and susceptibility to tapping panel dryness in Côte d’Ivoire using the 32 and 35 KDa lutoidic proteins
This study was carried out to identify protein markers with yield potential and susceptibility to tapping panel dryness (TPD). To achieve this goal, 11 clones, stimulated and non stimulated, yield and susceptibility to TPD were compared. Their lutoid fraction polypeptides were analysed using one and two-dimensional electrophoresis. Susceptibility to TPD appeared as a clonal trait which is not related...
May 2012
Effect of hormone treatments on deformed fruit development in pear
Fruit shape is one of the most important limiting factors in the quality of pear fruits. The effects of applied plant growth regulators on fruit shape have been determined in pear. The application of naphthalene acetic acid (NAA, 20 mg/L),indole-3-butyric acid (IBA, 30 mg/L), 6-benzalyamino acid (6-BA, 30 mg/L), cleaner production promotion unit (CPPU, 10 mg/L), gibberrelin (GA4+7, 3%)...
May 2012
Characterization of p-nitrophenol degrading actinomycetes from Hundung limestone deposits in Manipur, India
p-Nitrophenol (PNP), a major nitroaromatic xenobiotic is released into the environment as a result of its widespread use and as a breakdown product of organophosphate (OP) agricultural pesticides such as parathion and methyl parathion. It is highly toxic to soil microflora and other non-target organisms. Of several Microbial Biotechnology Research Laboratory (MBRL) strains isolated from limestone deposits in Ukhrul,...
May 2012
Application of fluorescence in situ hybridization (FISH) to the analysis of sulfate reducing bacterial community in an oily bench scale reactor
Advances in the field of genomics and meta-genomics have led to rapid and accurate strategies for the monitoring of microbial biodiversity and have revealed its potential for biotechnological applications. In this study, fluorescent in situ hybridization (FISH) as a culture-independent molecular approach using specific CY3-labelled oligonucleotide probes was used to study the dynamics of the sulfate reduction...
May 2012
Biochemical properties and microbial analysis of honey from North-Western regions of Iran: Seasonal effects on physicochemical properties of honey
May 2012
Effects of debranning and ultra-fine grinding on the quality of flour and cookies from blue wheat
The effects of debranning levels of 4 and 8% and ultra-fine grinding on physico-chemical, rheological, and pasting properties of wheat flour obtained from blue wheat, as well as cook quality, were investigated. The process of debranning decreased ash (from1.89 to 1.62%) and wet gluten content (from 44.68 to 38.81%), but increased the gluten index of wheat flours (from 12.95 to 17.82). Debranning had positive effects on...
May 2012
A chitosanase purified from the snail of Achatina fulica
A chitosanase from the snail of Achatina fulica was purified 18.27-fold with 0.68% recovery of protein and 12.53% recovery of enzymatic activity by phenyl-sepharose column chromatography, diethylaminoethanol (DEAE)-sepharose column chromatography and Sephacryl S-300 gel filtration. The molecular mass of chitosanase was 72 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...
May 2012
Antibacterial effects of zinc oxide nanoparticles on Escherichia coli K88
This study was conducted to evaluate the antibacterial effects of zinc oxide nanoparticles in vitro. Escherichia coli K88 was chosen as an indicator of pathogenic bacteria, because it could cause diarrhea in both children and in early-weaned piglets. In this study, the characterization of the nanoparticles was examined. Antibacterial activities against E. coli K88 were evaluated by...
May 2012
Desiccation-induced changes in viability, lipid peroxidation and antioxidant enzyme activity in Mimusops elengi seeds
Intermediate seeds of Mimusopsis elengi showed obvious membrane lipid peroxidation during desiccation. When the moisture content (MC) decreased from initial 41.8 to 6.1%, seed viability significantly decreased from 100 to 23%, consorted with activity changes of a few anti-oxidative enzymes. The activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR)...
May 2012
Resveratrol protects against hazardous effects of 2-bromopropane on maturation of mouse oocytes, fertilization and fetal development
2-Bromopropane (2-BP) is regularly used as an alternative to ozone-depleting cleaning solvents. Previously, we reported the cytotoxic effects of 2-BP on oocyte maturation and subsequent pre- and post-implantation development, both in vitroand in vivo. In the current study, we further demonstrate that these hazardous effects are suppressed by resveratrol, a grape-derived phytoalexin with known antioxidant and...
May 2012
The seasonal distribution of airborne fungi in two hospitals in Istanbul
Changes in fungal concentrations depend on seasonal and environmental conditions. The aim of this study was to determine the seasonal distributions of the fungal flora in Dr. Siyami Ersek Chest, Heart and Vascular Center Education and Research Hospital (SEH) and Kartal Yavuz Selim State Hospital (KYSH) in Ä°stanbul. Samples were collected seasonally from different stations (microbiology laboratory, toilet,...
May 2012
Effect of similar feeding regime on growth and body composition of Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) under mono and polyculture
Growth performance and body composition of yearling Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) was evaluated in semi-intensive (mono and polyculture) systems for 90 days. Prior to stocking, all ponds were fertilized with organic and inorganic manures. This application was repeated every two weeks throughout the study period. Supplementary feed containing 35% protein was applied daily...
May 2012
Genetic diversity in Indian sub-continental landrace cultivars of the genus Triticum L.
Narrowing genetic diversity is a limiting factor in wheat breeding. Popularity of semi dwarf cultivars, developed after green revolution, has resulted in genetic erosion as they replaced indigenous cultivars derived from landraces. These old cultivars have a wealth of useful genes that can be incorporated in the modern cultivars to improve their tolerance level against biotic and abiotic stresses. Genetic analysis of...
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