October 2013
DRE-binding Transcription factor (DREB1A) as a master regulator induced a broad range of abiotic stress tolerance in plant
Environmental stresses, such as drought, high salinity, and low and high temperature, affect plant growth. Abiotic stresses cause losses worth hundreds of million dollars of agricultural industry each year and extremely decrease crop productivity. Many genes are induced in response to environmental stresses. TheDREB1A gene is a stress-inducible DRE-binding transcription factor that strongly up-regulates many...
October 2013
Assessing genetic diversity of Hamdani sheep breed in Kurdistan region of Iraq using microsatellite markers
The genetic variation in Hamdani sheep in the Kurdistan region of Iraq was studied using 35 microsatellite markers. All 35 markers were found to be highly polymorphic. The mean number of observed alleles ranged from four at BMS1494 to twenty five at BM6444 and INRABERN185 loci. The polymorphic information content (PIC) values ranged from 0.208 for BMS1494 loci to 0.935 for INRABERN185 with an average value of 0.744. The...
October 2013
Antagonistic bioactivity of endophytic strains isolated from Salvia miltiorrhiza
The antibiotic-producing potential of endophytic populations from medical plant ofSalvia miltiorrhiza was examined. A total of 63 isolates was screened against five fungal and three bacterial species for the production of antimicrobial compounds. It showed that more isolates was antagonistic to fungi than to bacteria. One bacterial isolate named DX30 showed broad-spectrum of antagonistic activity against...
October 2013
Different effect of scion types on callusing in bench grafting
This study was carried out to determine the different effect of scion types on bench grafting. Rootstock 110R and Hafizali and Cardinal cvs. were used as a plant material. Scion types performed burst cutting-burst scion, burst cutting-swollen scion, and burst cutting-unburst scion. 21 days after grafting, all grafted cuttings were measured and recorded. The results indicate that discarded grafted-cuttings...
October 2013
In vitro regeneration of plantlets from unpollinated ovary culture in sweet orange (Citrus sinensis L. Osbeck)
Callogenesis and organogenesis of ovary of sweet orange (Citrus sinensis) cv. Blood red was carried on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of N6 benzyl adenine (BA), 1-napthaleneacetic acid (NAA) and 2,4-D. 1 mg/l BA + 0.5 mg/l NAA on MS medium was the most effective in callus induction and proliferation. Maximum number of shoots (11) was...
October 2011
Conditions for regeneration and transformation of Solanum tuberosum cultivars using the cotton glutathione S-transferase (Gh-5) gene
Our aim was to produce multiple stress-resistant plants containing the glutathione-S-transferase (Gh-5) auxin-regulated gene, which is related to the presence of the stress resistance protein and is expressed in the transgenic plant genome. We used the Agrobacterium-mediated transformation method. In all tested cultivars (Chubaek, Jasim, Jopoong, Namjak and Sumi), there were higher regeneration frequencies...
October 2011
Determining the effects of Trichoderma harzianum and some mycorrhizal fungi on plant growth and against Rhizoctania solani Kühn in Lilium under in vivo conditions
This work represents a research of non-sugar matter (the content of α- amino nitrogen, potassium and sodium) in the root, and their effect on the recoverable sucrose quantity of three genotypes (Esprit, Belinda and Chiara) of sugar beet from vegetation areas of different sizes. They were grown by standard agrotechnical measures for sugar beet in the densities of 80.000, 100.000 and 120.000 plants per hectare. In...
October 2011
Comparison between different bio-treatments of a hydrocarbon contaminated soil from a landfill site
We investigated the bio-remediation of a hydrocarbon contaminated soil pile that was slated for landfill disposal, by utilising laboratory based-soil microcosms. The objective was to accelerate the reduction of soil total petroleum hydrocarbon (TPH) to levels that could potentially allow the soil to be used outside a landfill site. Soil TPH content reduced by 57% over a 2 year period from 15,800 to ~6,800 mg...
October 2011
Screening of complex thermophilic microbial community and application during municipal solid waste aerobic composting
Two thermophilic bacteria HP83 and HC181 with fast growth and good decomposition ability of protein and cellulose were separated from 48 samples such as landfill, composting and animal manure by identical medium and activity assaying. Complex microbial community HP83 and HC181 were applied during municipal solid waste aerobic composting that was carried out in a composting reactor under controlled conditions. The...
October 2011
One step purification of biological active human interleukin-2 protein produced in yeast (Pichia Pastoris)
Pharmacological importance of recombinant human interleukin-2 protein has increased the demand to establish effective, reliable and cost effective chromatography method for its production and purification on large scale. One step mimetic ligand affinity chromatography method for purification of mutated human recombinant interleukin-2 (mrhIL-2), from Pichia Pastoris is described with higher yield then reported...
October 2011
Non-sugar matter as an indicator of technological value in different sugar beet genotypes
This work represents a research of non-sugar matter (the content of α- amino nitrogen, potassium and sodium) in the root, and their effect on the recoverable sucrose quantity of three genotypes (Esprit, Belinda and Chiara) of sugar beet from vegetation areas of different sizes. They were grown by standard agrotechnical measures for sugar beet in the densities of 80.000, 100.000 and 120.000 plants per hectare. In...
October 2011
Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves
Recently, there has been increasing interest in Ficus deltoidea (Moracea) due to its chemical composition and the potential health benefits. The leaves of the plant have been suggested to have potential antidiabetic effects. Inhibition of carbohydrate-hydrolysing enzymes, such as α-glucosidase and α-amylase is one of the therapeutic approaches to control postprandial hyperglycemia. In...
October 2011
Enzyme immunoassay measurements of the molting hormone in different post-embryonic stages of two mosquito species, Culex pipiens and Culiseta longiareolata
Due to their large geographical distribution, abundance and harmfulness, Culex pipiens L. and Culiseta longiareolata Macquart are the two most remarkablemosquito species in Algeria. The nature and the level of ecdysteroids from whole body extracts of different post-embryonic stages of the two mosquito species was made by an enzyme-immunoassay (EIA) using two...
October 2011
The reactivity of CysF9[93]β sulphydryl group of des-HisHC3[146]β human haemoglobin A
The pH dependence of the second order rate constant of the reaction of 5,5′-dithiobis(2-nitrobenzoate) (DTNB) with CysF9[93]β sulphydryl group of human haemoglobin A at 50 mmol dm-3 is complex. The removal of the terminal HisHC3[146]β of haemoglobin A by enzymatic cleavage with carboxypeptidase A breaks the salt bridge between HisHC3[146]β and AspFG1[94]β and reduces the strain on...
October 2011
Effects of active, inactive and compounded Saccharomyces cerevisiae on growth-related hormones and performance of Japanese quails (Coturnix Japonica)
The objective of this research was to determine the effects of active, inactive and compounded Saccharomyces cerevisiae (SC) as natural feed additives on growth performance, visceral organs weight, insulin, thyroxin and growth hormone of Japanese quails. One day old Japanese quails allocated in 4 treatments by 4 replicates and 12 birds per pen. The experimental diets consisted of a basal diet without additive...
October 2011
Antitumor activity of baicalein on the mice bearing U14 cervical cancer
Baicalein is one of the major constituents of Scutellaria baicalensis, and some cancer cells could be inhibited by it according to some studies in recent years. Mice tumor models of U14 cervical cancer was established in our study, baicalein of high and low dose (40 and 20 mg/kg, respectively) were given orally to mice. The tumor inhibition rates and the thymus (spleen) weight index were studied, the...
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