African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12212

Table of Content: 3 December 2008; 7(23)

December 2008

Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima)

    Several lowland NERICAs (New Rice for Africa) were derived from crosses between IR64 (an Oryza sativa subsp. indica variety) and Tog5681 (an Oryza glaberrima variety) that possess useful traits adapted to lowland conditions in West Africa. The proportion of parental genomic contribution and extent of genetic differences among these...

Author(s):     Marie Noelle Ndjiondjop, Kassa Semagn, Moussa Sie, Mamadou Cissoko, Blandine Fatondji and Monty Jones

December 2008

Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes

    The phylogenetic relationships of 13 Jatropha genotypes from different parts of the India were analysed using 34 polymerase chain reaction (PCR) markers (20 random amplified polymorphic DNAs (RAPDs) and 14 inter simple sequence repeats (ISSRs)). Amplification of genomic DNA of the 13 genotypes, using RAPD analysis, yielded 107 fragments that could be scored, of which 91 were...

Author(s):     Shweta Gupta, Mani Srivastava, G. P. Mishra, P. K. Naik, R. S. Chauhan, S. K. Tiwari, Meetul Kumar and Raghwendra Singh

December 2008

Genotoxicity of Chlorpyrifos, Alpha-thrin, Efekto virikop and Springbok to onion root tip cells

    The pesticides, chlorpyrifos, Alpha-thrin, Efekto virikop and springbok were assessed for cytotoxicity and genotoxicity in the onion root tip assay. Onion seeds were germinated on moistened filter paper in petri dish at room temperature until radicles appeared. Germinated seeds were exposed to three concentrations of each pesticide for 20 h. About 1 – 2 mm length of...

Author(s):   Asita Okorie Asita and Rets’elisitsoe Makhalemele

December 2008

Cloning, sequencing and expression of a novel xylanase cDNA from a newly isolated Aspergillus awamori in Pichia pastoris

    A strain SH 2016, capable of producing xylanase, was isolated and identified asAspergillus awamori,based on its physiological and biochemical characteristics as well as its ITS rDNA gene sequence analysis. A xylanase gene of 591 bp was cloned from this newly isolated A. awamori and the ORF sequence predicted a protein of 196 amino acids with a molecular mass about 21...

Author(s):     Yinan Yang, Kuixian Shan, Lifeng Ping and Jingmei Lu

December 2008

Host range and some characterization of Tobacco streak virus isolated from lettuce in Iran

  Lettuce (Lactuca sativa) is one of the most common vegetable planted in the world, so the identification of its viral disease is important because this kind of pathogen causes the loss of quantitative and qualitative characteristic of lettuce. Tobacco streak virus(TSV) is an ilarvirus with quasi-isometric particles, 27 - 35 nm in diameter. It has a wide host range and occurs...

Author(s):

December 2008

Rauwolfia serpentina: Protocol optimization for in vitro propagation

    Rauwolfia serpentina L. Benth. ex. Kurz. (Apocynaceae) a woody perennial shrub, possess alkaloids namely reserpine, ressinamine and yohmbine, and used to cure various neurological ailments. The present investigation is an effort to establishRauwolfia for micro-propagation and sub-culturing. The shoots and leaves were used as explants and cultured on MS media...

Author(s):   Richa Bhatt, Mohd Arif, A. K. Gaur and P. B. Rao

December 2008

Determination of pollen viability, germination ratios and morphology of eight apricot genotypes

    Turkey is a leading apricot producing country, with the majority of dried apricot production centered in Malatya. Currently, there are breeding programs to develop superior apricot cultivars for several different utilizations.  Determining the components of reproduction biology is critical for optimizing yields from apricot orchards and is therefore important for breeding...

Author(s):   Bayram Murat Asma

December 2008

Assessment of the hepatic effects, heamatological effect and some phytochemical constituents of Ximenia americana (Leaves, stem and root) extracts

    The aqueous extracts of the leaf, stem bark and root of Ximenia americana was evaluated for its phytochemical constituents. A study was also conducted with 20 albino rats (Wistar strain) weighing between 100-130 g to access for hepatic effect and haematological effect. The extracts were administered every day for a period of 21 days. Group 1 was the control animals...

Author(s):   D. B. James, A. O. Owolabi, H. Ibiyeye, J. Magaji and Y. A.  Ikugiyi

December 2008

Induction and multiplication of callus from endosperm of Cycas revoluta

    The usage of medicinal plants in traditional medication has gained the attraction from global and local markets, mainly to cure diseases or simply for health maintenance. Callus cultures were initiated from the endosperm of the medicinal plant Cycas revoluta, cultured on half-strength Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose and various...

Author(s):   Anna Ling Pick Kiong, Yeo Shu Thing, Jualang Azlan Gansau and Sobri Hussein

December 2008

Relationships between yield and some yield components in Pea (Pisum sativum ssp arvense L.) genotypes by using correlation and path analysis

    This research was carried out Yuzuncu Yil University, Agricultural Faculty experimental field in 2002-03 and 2003-04. The experiment was conducted to determine the relationship among yield and some yield components using correlation and path coefficient analysis. In the experiment 12 pea genotypes were used. The experiment was designed randomized complete blocks design with...

Author(s):   Necat Togay, Yesim Togay, Bunyamin Yildirim and Yusuf Dogan

December 2008

Effect of tillage on the efficacy of CGA362622 on weed control in maize

    A field trial was conducted to assess the performance of CGA362622 (trifloxysulfuron sodium), a sulfonylurea based herbicide formulated with ametryn (N-ethyl-N-(1- methy (ethyl)-6-(methylthio)-1,3,5-triazine-2, 4-diamine) on weed control in maize at the Faculty of Agriculture, University of Benin, Benin City, Nigeria. The herbicide was applied pre-emergence at 200 g ai/ha and...

Author(s):   J. U. Chokor, C. E. Ikuenobe and C. N. Odoh

December 2008

Biodegradation of aliphatic, aromatic, resinic and asphaltic fractions of crude oil contaminated soils by Pleurotus tuber-regium Fr. Singer - a white rot fungus

  The use of chemical fertilizer and animal manure on crude oil reduction during biodegradation with Pleurotus tuber-regium was investigated. The co-substrates and inocula types used for the investigation were shredded banana leaf blades and sawdust of Albezia and sclerotium and spawn of the fungus, respectively. The reduction of total petroleum hydrocarbons was higher in treatments...

Author(s):   Erute Magdalene Ogbo and John Ariyo Okhuoya

December 2008

The acute toxicity of lead nitrate on Daphnia magna Straus

    In this study the acute toxicity of lead nitrate (Pb(NO3)2) to Daphnia magna Straus was investigated in a static bioassay. After 24 h the mobility of daphnids were examined and immobile ones were counted. The 24 h EC50 of lead nitrate to D. magna was found as 0.44 mg/L. According to Behrens-Karber method the 24 h EC50  of lead was found as 0.51...

Author(s):     Ahmet Altındağ, Mehmet Borga Ergönül, Sibel Yigit and Özge Baykan

December 2008

Heavy meal decontamination of polluted soils using Bryophyllum pinnatum

    Phytoremediation was carried out with Bryophyllum pinnatum as a means of cleaning up soils contaminated with heavy metals. Maximum levels of metals accumulated were Ni (11.91 mg/kg), Pb (399.90 mg/kg), Cr (32.48 mg/kg), V (5.81 mg/kg) and Cd (3.12 mg/kg) and this occurred in the 4th month of study. Results of this study indicate that B. pinnatum is an...

Author(s):   S. A. Odoemelam and R. A. Ukpe

December 2006

Haematological and serum biochemical response of growing rabbit bucks fed dietary fumonisin B1

    An experiment was conducted with 48 crossbred rabbits (49 days old) averaging 757.5 ± 0.50 g to assess haematological and serum biochemical response of growing rabbits exposed to varied levels of dietary fumonisin of 0.1, 5.0, 7.5 and 10 mg fumonisin B1/kg diet containing in diets 1 (control) 2, 3 and 4 respectively, for a period of 12 weeks. Blood samples were...

Author(s):   Ewuola, E. O. and Egbunike, G. N.

December 2008

Hatchability of chicken eggs as influenced by turning frequency in hurricane lantern incubator

    An experiment was conducted to determine the influence of turning frequency of chicken eggs on hatchability in hurricane lantern incubator. There were four treatments in which eggs were not turned in treatment one (control), those in treatment two were turned once daily (morning), treatment three turned twice daily (morning and afternoon) while treatment four were turned...

Author(s):   S. S. Abiola, A. O. Afolabi and O. J. Dosunmu

December 2008

Ligninolytic enzyme activities in mycelium of some wild and commercial mushrooms

    Lignin is probably one of the most recalcitrant compounds synthesized by plants. This compound is degraded by few microorganisms. White-rot fungi have been extensively studied due to its powerful ligninolytic enzymes. In this study, ligninolytic enzyme activities of different fungal species (six commercial and 13 wild) were investigated in solid and liquid culture media. It...

Author(s): Erbil Kalmış, İhsan Yaşa, Fatih Kalyoncu, Barış Pazarbaşı and Ali Koçyiğit

December 2008

Perceptions and attitudes of geography teachers to biotechnology: A study focusing on genetically modified (GM) foods

    This study reports the perceptions and attitudes of geography teachers towards biotechnology and genetically-modified (GM) foods in Turkey. A survey was conducted with secondary school geography teachers attending teacher workshops in various parts of the country in 2008 and was responded to by 78 teachers from 31 different provinces. The study not only revealed important...

Author(s):   Ali Demirci

December 2008

Iodine contents of some selected roots/tubers, cereals and legumes consumed in Nigeria

      Some selected staple foods: tubers, cereals, and legumes, grown, sold and consumed in Ijebu-North Local Government area, Nigeria were evaluated for their iodine content using standard methods.  Among the tubers, Ipomea batata (sweet potatoe, red species) was observed to have the highest iodine content (311.33±8.52 mgI) while the...

Author(s):   Salau, B.A., Ketiku, A.O., Ajani, EO, Ezima, E.N., Idowu, G.O. and Soladoye, M.O.    

December 2008

Quality assessment and potential utilization of high amylolytic Nigerian maize cultivars

    This study was carried out to compare the qualities of two acclaimed Nigerian amylolytic maize cultivars; SPMAT and TZEE*TZEE-W*DEMARSCUS*TZEE-W with barley, red and white sorghum. Results obtained (P ≤ 0.05) showed marked differences in the properties of the two maize cultivars compared to barley, white and red sorghums. Suggestions on the application of these cultivars...

Author(s):   Awoyinka, O. A., and Adebawo, O. O.

December 2008

Hydrodynamic behaviour and biochemical characterization of a simple custom expanded bed column for protein purification

    Hydrodynamic behaviour and biochemical characterization of a simple custom expanded bed column made by Nanobiotechnology Group (NBG) was compared with a commercial expanded bed contactor. Hydrodynamic characteristics of the columns (Daxl and Bo) with various settled bed height (SBH = 5 - 10 cm, increment of 1 cm) along with variety of column diameters (1.0, 1.6, 2.0 and...

Author(s):   M. H. Shahavi, G. D. Najafpour and M. Jahanshahi

December 2008

The use of heavy metals load as an indicator of the suitability of the water and fish of Ibiekuma Stream for domestic and consumption purposes

    The levels of Cd, Cr, Cu, Pb and Zn in the water and fish from Ibiekuma Stream, Ekpoma, Nigeria were determined at two locations (Upstream and Downstream) in order to ascertain their suitability for consumption and other domestic uses. Atomic Absorption Spectrophotometry was used to measure the metal levels. Seasonal (dry and wet seasons) levels were also determined. The...

Author(s):   Obasohan, E. E.

December 2008

In vitro antifungal activity of methanol extracts of some Indian medicinal plants against pathogenic yeast and moulds

    The methanol extract of 9 Indian medicinal plants belonging to 9 different families were evaluated for in vitro antifungal activity against some yeasts including Candida albicans(1) ATCC2091, C. albicans (2) ATCC18804, Candida glabrata NCIM3448, Candida tropicalis ATCC4563, Cryptococcus luteolus ATCC32044,...

Author(s):     Jigna Parekh and Sumitra Chanda