African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 3 January 2011; 10(1)

January 2011

Effects of green manure crops and mulching technology on reduction in herbicide and fertilizer use during rice cultivation in Korea

Green manure crops are primarily used in environmentally friendly agricultural practices to reduce the application of chemical fertilizer and herbicide. In this study, a two-year field experiment was conducted to evaluate the effects of paper and plastic mulching with hairy vetch alone or in combination with barley on weed control and rice yield. In addition, treatment effects on soil redox potential (Eh) and the...

Author(s): Weon Tai Jeon, Bong Su Choi, Samy A. M. Abd El-Azeem and Yong Sik Ok  

January 2011

One step method of plantlet regeneration in Trichosanthes dioica Roxb.: An approach towards cost effective and shorter protocol

In order to reduce the cost and time of in vitro raised plants of Trichosanthes dioicaRoxb., a minimal medium has been formulated by substituting costly growth regulators from the medium with a cost effective constituent, the coconut milk. A semisolid Murashige and Skoogs’s medium supplemented exclusively with 15% coconut milk showed the highest percentage of plantlet regeneration (99%) in the...

Author(s): Rashmi Komal

January 2011

A study on the qualitative and quantitative traits of barley (Hordeum vulgare L.) and narbon vetch (Vicia narbonensis L.) in intercropping and sole cropping system under the interference and control of weeds in dry land farming conditions of Iran

In order to investigate the effect of intercropping and sole cropping of barley and narbon vetch with weed control and interference conditions on their qualitative and quantitative traits, this experiment was conducted with 5 levels of intercropping (seed contribution): sole cropping of narbon vetch, 75:25 (narbon vetch : barley), 50:50, 25:75 and sole cropping of barley. Two levels of weed control and weed interference...

Author(s): Khosro Azizi, Alireza Daraei Mofrad, Saeid Heidari, Majid Amini Dehaghi and Danial Kahrizi

January 2011

Attitudes of secondary school students towards environmental and sustainable development issues: A case study from Turkey

As a consequence of the industrial revolution, countries around the world began to exploit natural resources more than ever before without regard for maintaining a balance between development and nature. The problems stemming from these human actions has compromised the ability of subsequent generations to meet their needs from the earth and in the last few decades, have obliged them to re-structure their educational...

Author(s): Süleyman Ä°ncekara and Fikret Tuna

January 2011

Pathogenicity of local Metarhizium anisopliae var. acridum strains on Locusta migratoria migratorioides Reiche and Farmaire and Zonocerus variegatus Linnaeus in Senegal

Locusts and grasshoppers are the most important economical threat in the sahelian agricultural system. Principal control strategies of these pests are synthetic chemicals which are, however, harmful to the environment and human health. Metarhizium anisopliae based biopesticide Green Muscle IMI330189 has been recently developed for the control of locusts and grasshoppers. In this paper, we...

Author(s): Niassy, S., Diarra, K., Ndiaye, S. and Niassy, A.

January 2011

Influence of high intensity ultrasound with different probe diameter on the degree of homogenization (variance) and physical properties of cow milk

The main goal of this research is to analyze the influence of ultrasonic probe diameters (7 and 10 mm) of high-intensity ultrasound with constant frequency (30 kHz) on the degree of homogenization (variance) of cow milk. Influence of different probe diameters on the physical properties of cow milk was also tested. Changes in temperature, pH and density were measured under the following operational conditions of the...

Author(s): Bosiljkov, T., Tripalo, B., Brnčić, M., Ježek, D., Karlović, S. and Jagušt, I.  

January 2011

Efficacy of lactic acid bacteria in the reduction of trimethylamine-nitrogen and related spoilage derivatives of fresh Indian mackerel fish chunks

  Different strains of lactic acid bacteria (LAB) such as Pediococcus acidilactici,Pediococcus pentosaceous, Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus helveticus were procured from the NCL (National Chemical Laboratory), Pune, India. These LAB cells were individually coated on the dressed fresh mackerel fish...

Author(s): Kannappan Sudalayandi and Manja

January 2011

Ethanol production from Washingtonia robusta fruits by using commercial yeast

In this study, ethanol production from fruits of Washingtonia robusta was investigated.  Effects of different contact times (1 - 24 h), pH (2 - 12) temperature (20, 30, 40, 50ËšC) and autoclave pretreatment (121ËšC, 10 min, 1.2 atm) were also studied to improve the solubility of reducing sugar in fruits. Baker’s yeast (Saccharomyces cerevisiae) was used for fermentation of that reducing sugar....

Author(s): Mehmet Ali Mazmanci

January 2011

Antispasmodic and bronchodilator activities of Taxodium mucronatum Ten leaf extract

The relaxant effects of the Taxodium mucronatum Ten leaf hexane extract on intestinal and tracheal smooth muscle were evaluated in vitro by testing spontaneous contractions of rabbit jejunum and agonist-induced contractions of guinea pig ileum and rat trachea. The extract produced a concentration-dependent relaxation of the spontaneous contractions of rabbit jejunum (EC50: 4.9 ± 0.5...

Author(s): Alma Rosa Cortés-Arroyo, Adriana M. Domínguez-Ramírez, Martín Gómez-Hernández, José R. Medina López, Marcela Hurtado y de la Peña and Francisco J. López-Muñoz

January 2011

Bauhinia purpurea leaves’ extracts exhibited in vitro antiproliferative and antioxidant activities

The antiproliferative and antioxidant activities of various extracts of the leaves ofBauhinia purpurea were studied using in vitro standard assays. The aqueous and chloroform extracts successfully inhibited the proliferation of all cancer cells while the methanol extract inhibited the proliferation of all cells except the CEMss cells when assessed using the...

Author(s): Z. A. Zakaria, M.S. Rofiee, L. K. Teh, M. Z. Salleh, M. R. Sulaiman and M. N. Somchit

January 2011

Medium optimization for endochitinase production by recombinant Pichia pastoris ZJGSU02 using response surface methodology

Plackett-Burman design and response surface methodology were employed to optimize the medium components for endochitinase production by Pichia pastoris. A Plackett- Burman design of seven factors with 12 runs was applied to evaluate the effects of different medium components. Yeast extract, oleic acid and Tween-80 were found to have significant influence on endochitinase production. The optimal concentrations of...

Author(s): Ping Yu, Yuan Yan and Yun-ping Tang

January 2011

Cloning of a gene encoding glycosyltransferase from Pueraria lobata (Wild.) Ohwi and its expression in Pichia pastoris

The key enzyme of puerarin biosynthesis in Pueraria lobata (Willd.) Ohwi was unclear but may involve glycosylation. To investigate the regulation of puerarin biosynthesis, a putative UDP-dependent glycosyltransferase (UGT) gene, PlUGT1 was isolated from P. lobata root, which contained abundant puerarin. PlUGT1encoded 480 deduced amino acid residues with a conserved UDP-glucose-binding domain,...

Author(s): Wenling Zhou, Yinghua Wang, Gang Chen, Min Lü, LixiaYang, Xiaoxiang Hu, Haihang Li and Ling Li

January 2011

Phytotoxic, Antibacterial and Haemagglutination activities of the aerial parts of Myrsine africana L.

The crude methanolic extract and various fractions derived from the aerial parts ofMyrsine africana were screened in vitro for possible phytotoxic, antibacterial and haemagglutination activities. Moderate phytotoxic activity (31.25 %) was observed against Lemna minor L at 1000 µg/ml by chloroform fraction (CHCl3). The crude methanolic extract and CHCl3 fraction showed good...

Author(s): Bashir Ahmad, Sadiq Azam, Shumaila Bashir, Ibrar Khan, Niaz Ali and M. Iqbal Chaudhary

January 2011

Isolation of fungal endophytes from Garcinia mangostana and their antibacterial activity

The objective of this study is to screen the antibacterial activity of endophytic fungi isolated from surface sterilized leaves and small branches of Garcinia mangostanaplant found in Indonesia. The crude extracts of ethyl acetate (EtOAc) of the 24 fermentation broths from 24 endophytic fungi were tested for their antibacterial activity against Staphylococcus aureus ATCC 25923, Bacillus...

Author(s): Maksum Radji, Atiek Sumiati, Renita Rachmayani and Berna Elya

January 2011

Activities of amylase, trypsin and chymotrypsin of pancreas and small intestinal contents in the red jungle fowl and broiler breed

The digestive enzyme activities of the pancreas and small intestinal segments were examined in two breeds of chickens that differ in growth rate over the period of 1-day (1-d) to 4-months (120-d) of age. The total body weight (BW) of the red jungle fowl (RJF) increased slowly during the experiment, in contrast to the commercial broiler chicken (CBC) which showed markedly increase (P < 0.05) over the same experiment....

Author(s): Khalid K. Kadhim⊃, A. B. Z. Zuki⊃, M. M. Noordin⊃, S. M. A. Babjee⊃ and M.Zamri-Saad⊃