African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

Table of Content: 4 January 2010; 9(1)

January 2010

Molecular characterization of mulberry germplasm from Eastern Anatolia

Mulberry has an important quality for both horticultural production and sericulture in Turkey. Anatolia has an extensive potential for mulberry. On the other hand, these mullberry germplasm has not been adequately determined and relatedness among them has not been properly investigated. A total of 47 mullberry genotypes; 20, 14, 5, 4 and 4 from Erzincan, Elazığ, Diyarbakır, Malatya and Mardin provinces, respectively,...

Author(s): Ozrenk K., Gazioglu Sensoy R. I.,   Erdinc C., Guleryuz, M. and Aykanat, A.

January 2010

The effect of sowing date and row spacing on yield and yield components on Hashem chickpea variety under rainfed condition

In order to investigate the impacts of sowing date and row spacing on yield and yield components of Hashem chickpea variety, a field experiment was conducted in 2005 at farm of Dorood Faraman (Kermanshah-Iran). In this study, the sowing date in three level (6, 23 November and 6 December) and the row spacing in three level on rows (20, 30 and 40 cm) were evaluated with complete randomized block design in...

Author(s): Keyvan Shamsi

January 2010

Corn and potato starch as an agar alternative for Solanum tuberosum micropropagation

Potato single nodes were subcultured onto fresh MS medium gelled with 0, 1 and 2 g/l of agar + 40, 50 or 60 g/l of commercial corn and potato starch (CS or PS, respectively). After 4 weeks of culture, the pH of medium supplemented with 50 or 60 g/l of CS or 60 g/l of PS was significantly decreased to 3.91 - 4.00. This reduction coincided with a significant increment in electric conductivity (EC) which was 172 - 214...

Author(s): Mohamed, M. A. H., Alsadon, A. A. and Al Mohaidib, M. S.

January 2010

Effects of Bacillus thuringiensis CRY1A(c) d-endotoxin on growth, nodulation and productivity of beans [Phaseolus vulgaris (L.) and siratro (Macroptilium atropurpureum DC.)]

The recent introduction of Bt maize and Bt cotton transgenic crops into Africa has raised concerns on their potential short and long-term ecological effects on the environment. The effects of Bacillus thuringiensis (Bt) Cry1A(c) d-endotoxin on the growth, nodulation and productivity of two leguminous plants grown in clay soil were evaluated. Bt Cry1A(c) d-endotoxin from a local B. thuringiensis isolate (ICIPE...

Author(s): H. M. Makonde, F. K. Lenga, D. Masiga, S. Mugo and H. I. Boga

January 2010

Effects of grafting on carbohydrate accumulation and sugar-metabolic enzyme activities in muskmelon

Carbohydrates are the most important biochemical compounds in determining the quality of muskmelon fruit, and their accumulation in the muskmelon (Cucumis melo L.) fruits as substrate tub plants in solar greenhouse with different scion/rootstock graft combinations were studied. The results indicated that Zhongmi1/Shengzhen1 graft combination (GS) reduced all kinds of main sugars contents except galactinol and...

Author(s): Yifei Liu, Tianlai Li, Hongyan Qi, Jiyao Li and Xiangyu Yin

January 2010

Effect of soil water stress on yield and proline content of four wheat lines

This field study was conducted to evaluate the effect of drought stress after anthesis on proline accumulation and wheat yield during 2008 at Moghan region. Four lines of bread wheat (N-82-9, N-83-5, N-84-12 and N-85-20) were evaluated into contrasting water regimes (well-watered and drought stressed after anthesis). The trial was carried out in a 4 × 2 factorial experiment based on complete block design with 3...

Author(s): M. Johari-Pireivatlou

January 2010

Decolourisation of chemically different dyes by enzymes from spent compost of Pleurotus sajor-caju and their kinetics

A total of eight dyes from the triphenylmethane, azo and polymeric/heterocyclic dye group were decolourized by enzyme cocktail extracted from five month old spent compost of Pleurotus sajor-caju with lignin peroxidase as the main enzyme. The percentage of decolourisation for tryphan blue, amido black, remazol brilliant blue R (RBBR) and bromophenol blue ranged between 80 - 90% after 4 h reaction. However, the...

Author(s): Avneesh D. Singh, Vikineswary Sabaratnam, Noorlidah Abdullah, Annuar, M. S. M. and Ramachandran, K. B.

January 2010

Biodegradation of dodecylbenzene solfonate sodium by Stenotrophomonas maltophilia Biofilm

Immobilization for microbial cultures has proved to be advantageous in municipal and industrial sewage treatment because of high degradation efficiency and good operational stability. In this survey, a bacterial strain was isolated from activated sludge that utilized branched anionic surfactants (BAS) as a sole carbon source. Identification of isolated strain was from 16S rRNA sequencing method. The immobilized cells on...

Author(s): Hosseini Farzaneh, Malekzadeh Fereidon, Amirmozafari Noor and Ghaemi Naser

January 2010

Thermoactive cellulase-free xylanase production from alkaliphilic Bacillus strains using various agro-residues and their potential in biobleaching of kraft pulp

The four bacterial strains were isolated on media containing xylan and screened for xylanase activity. The bacterial strains (Ag12, Ag13, Ag20 and Ag32) were characterized based on morphological, biochemical and physiological characters and identified as belonging to the genus Bacillus. The effects of different factors such as pH (7.0 – 10.0), temperature (25.0 – 50.0°C) and inexpensive...

Author(s): Cem Azeri, Abdurrahman U. Tamer and Mustafa Oskay

January 2010

The effects of smoking on the nutritional qualities and shelf-life of Clarias gariepinus (BURCHELL 1822)

The effects of smoking on the chemical, microbiological and sensory qualities of a commercially important freshwater fish species, Clarias gariepinus stored at 4°C for 28 days were investigated. The fish samples were collected from a research pond of Lagos State University, Lagos, Nigeria and Badagry Market, Lagos, Nigeria. The chemical profiles of the fresh and smoked fish were analyzed while the...

Author(s): C. A. Kumolu-Johnson, N. F. Aladetohun and P. E. Ndimele

January 2010

Safety evaluation of polyphenol-rich extract from bamboo shavings

In this paper, a number of acute and subchronic toxicological tests were documented to evaluate the safety of a polyphenol-rich ethanol extract of bamboo shavings (EEBS). (i) Acute toxicity test: The oral maximum tolerated dose (MTD) of EEBS was above 20 g/kg body weight for mice, thus the amount can be seen as practically non-toxic. (ii) Mutagenicity test: No mutagenicity was detected according...

Author(s): Jinyan Gong, Xiaoqin Wu, Baiyi Lu and Ying Zhang

January 2010

Effect of Ramadan fasting on glucose, glycosylated haemoglobin, insulin, lipids and proteinous concentrations in women with non-insulin dependent diabetes mellitus

The objective of this study was to investigate the effect of Ramadan fasting on body mass index (BMI) and on certain biochemical parameters of serum in women patients with non-insulin dependent diabetes mellitus. Sixty-six subjects from 3 regions located in the west of Algeria participated in this study. All participating patients were studied a week before Ramadan and at the third week of Ramadan fasting. No...

Author(s): Ait saada, D., Selselet attou, G., Belkacemi, L., Ait chabane, O., Italhi, M., Bekada, A. M. A. and Kati, D.

January 2010

Rare and unexpected beta thalassemic mutations in Qazvin province of Iran

About 13 beta-globin mutations encompass 70 - 90% of mutation spectrum in Iran. These mutations are called common beta-globin mutations. The rest are rare or unknown mutations. The objective of this study was to identify and describe rare or unknown beta-globin mutations in Qazvin province. EDTA-containing venous blood samples were collected from 100 patients with transfusion-dependent beta-thalassemia from the...

Author(s): Mohammad Reza Sarookhani and Mohammad Hosein Ahmadi

January 2010

The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114

Many methods for plasmid purification have been developed, and the whole process must be designed to remove the host RNA, protein, genomic DNA and endotoxin. Currently, plasmid is mostly purified by time-consuming chromatographies. As an alternative, a new plasmid purification technology with cetyltrimethylammonium bromide (CTAB) is described. After lysis with alkali, the CTAB was directly titrated into the supernatant...

Author(s): Zhang, Q., Yuan, W. F., Sun, H. C., Hou, S. H. and Zhu, H. F.

January 2010

Study of aqueous extract of three medicinal plants on cell membrane–permeabilizing and their surface properties

The aim of this study was to evaluate the effect of aqueous extract of three medicinal plants, Artemisia dracunculus L, Cuminum cyminum L and Heracleumpersicum Desf, which contain saponins on biological membrane. Also in this study, some of their physicochemical properties were studied. At the first step, the aqueous extract of the plants were prepared, using maceration and then the...

Author(s): Gholamreza Dehghan Noudeh, Fariba Sharififar, Mehdi Khatib, Effat Behravan and Masoud Ahmadi Afzadi