March 2012
Progress in structural analysis of glycosaminoglycans and their applications in biomaterials
The rising interest in the application of glycosaminoglycans (GAGs) is one of the main reasons for exploring different species and optimizing the extracting conditions of various GAGs in the last decade. Recent research data on GAGs have suggested that they have many new biological functions such as anti-atherogenesis, anticoagulation, prevention and cure of arthritis, morphogenesis and cell division. They...
March 2012
Changes in germination characteristics and seedling growth between storage and non-storage of primed tall fescue seed
In seed priming studies, seeds usually are sown directly in the field or stored for short periods of time. The objective of this study was to determine the effects of storage on the primed seed of tall fescue. Hydropriming and osmopriming methods were used in this study to prime the seeds. The water osmotic potential for osmopriming was between -1.5 and -2.2 MPa. After drying, the seeds were first...
March 2012
Genetic diversity and identification of variety-specific AFLP markers in fenugreek (Trigonella foenum-graecum)
Fenugreek (Trigonella foenum-graecum), an important spice crop belongs to the family Fabaceae. India is the largest producers as well as the largest exporter of fenugreek. Five common fenugreek varieties of India were included in this study for molecular analysis. For randomly amplified polymorphic DNA (RAPD) analysis, a total of 47 bands in the size range of 200 to 5000 bp were produced by examining...
March 2012
Genetic polymorphism in β-lactoglobulin gene of some sheep breeds in the Kingdom of Saudi Arabia (KSA) and its influence on milk composition
β-Lactoglobulin (β-Lg) is one of the most important proteins in mammals’ milk. It plays a crucial role in milk quality. The polymorphism of β-Lg gene can be used as a marker system. To analyze the genotype distribution of β-Lg gene in some sheep breeds reared in Taif region of Saudi Arabia and its influence on milk composition, sixty (60)animals...
March 2012
Tissue culture-induced alteration in cytosine methylation in new rice recombinant inbred lines
Zizania DNA introgression could induce a large number of genetic and epigenetic changes of the new rice recombinant inbred lines genome. In this present study, we employed inter-simple sequence repeat (ISSR) to further study the genetic and epigenetic changes that are induced by tissue culture. Changes induced by tissue culture are mostly epigenetic. One kind of methylation alterations is that...
March 2012
Polyethylene glycol (PEG)-mediated transformation of the fused egfp-hph gene into Pleurotus ostreatus
A transformation system for the basidiomycete Pleurotus ostreatus was established using PEG-mediated transformation. The homologous glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter and terminator regions of P. ostreatus were amplified and cloned onto the restriction site of the pUC19 vector. Enhanced green fluorescent protein-encoding gene (egfp) and a selection marker, the...
March 2012
Antibacterial activity of the endophytic fungi from medicinal herb, Macleaya cordata
A total of 48 endophytic fungal isolates were separated from the healthy roots ofMacleaya cordata R. Br. (Papaveraceae), a traditional medicinal herb mainly distributed in China. Nine distinct isolates (Macof01 to Macof09) were selected for further taxonomical identification by morphological traits and internal transcribed spacer (ITS) rRNA gene sequence analysis. Seven genera...
March 2012
Symbiotic responses to insoluble phosphorus supply in common bean (Phaseolus vulgaris L.): Rhizobia symbiosis
The common bean (Phaseolus vulgaris L.) is often grown under various environmental constraints in soil as well as phosphorus (P) deficiency. In worldwide soils, P massively exists in insoluble forms especially in acidic and basic soils, where it is greatly combined with Al, Fe and Ca ions hydroxide. In order to study the effect of insoluble phosphorus (IP) in hydroponic culture, two common bean...
March 2012
CRSM-38, a new high yielding coupled with CLCuV tolerance cotton (Gossypium hirsutum L.) variety
CRSM-38 is a high yielding and leaf curl virus tolerant cotton variety, developed in Pakistan. Hybridization and subsequent pedigree method’s selection work was carried out under natural field conditions at Cotton Research Station, Multan during the year 2001 and 2002 by using a virus tolerant strain (583-85/99) and a variety (FH-900). The new variety exhibited an average of 77.9% higher degree of...
March 2012
Oxidative damage and antioxidant response caused by excess copper in leaves of maize
Adequate copper (Cu2+) concentrations are required for plants, but at higher concentrations it can cause toxic effects. In this study, physiological parameters and antioxidant enzymes were assayed in the leaves of maize variety Zhendan958, which is one of the dominant cultivar of maize (Zea mays L.) in China due to its better traits in production, after the seedling was treated with 2 to 8 mM Cu for 96...
March 2012
Dormancy-breaking and salinity/water stress effects on seed germination of Atlas cypress, an endemic and threatened coniferous species in Morocco
Atlas cypress (Cupressus atlantica Gaussen) is an endemic coniferous medicinal species geographically restricted to the N’Fis valley river in the High Atlas Mountains in South-Western Morocco. Eight dormancy-breaking treatments, 5 NaCl concentrations, and 5 water potentials were tested on the germination of Altas cypress seeds that had...
March 2012
Assessment of washing procedure for determination some of airborne metal concentrations
This study was proposed to assess the suitability of washing technique to distinguish between airborne and soil borne several metal contaminants. For this reason, six plant species which grew under Mobarakeh Steel Company emissions were selected. Aluminum, iron, nickel, manganese, zinc, copper and lead concentrations were determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) in...
March 2012
Oil palm empty-fruit bunch application effects on the earthworm population and phenol contents under field conditions
The amounts of polyphenols at several stages of oil palm empty-fruit bunch (EFB) composting, the types of phenol compounds in several types of EFB composting processes and the effects of phenol compounds on EFB composting in an earthworm population were evaluated under field conditions. The amount of extractable phenols from decomposed EFB increased at the early stage of decomposition and decreased with...
March 2012
Diversity of isolates performing Fe(III) reduction from paddy soil fed by different organic carbon sources
This study examined microbial diversity of Fe(III)-reducing bacteria isolates in paddy soils amended with ferrihydrite and different organic carbon (C) sources. Results show that Fe(III) reduction rate and Fe(II) accumulation in soil enrichments amended with glucose were the greatest followed by pyruvate, lactate, acetate and control. ARDRA (amplified ribosomal deoxyribonucleic acid restriction...
March 2012
Isolation and characterization of Pseudomonas resistant to heavy metals and poly aromatics hydrocarbons (PAHs) from Persian Gulf sediments
The Persian Gulf is the most strategic waterway in the world due to its importance in the global oil transportation. Heavy metals and poly aromatics hydrocarbons (PAHs) are the most important pollutants in the Persian Gulf and other marine ecosystems. Our goal in this study is to isolate high resistant degrading species for copper and phenanthrene from Khur Mousa sediments (located in the North of the Persian...
March 2012
Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification
A recombinant Pichia strain GS115-Ch-Glu expressing both β-glucosidase and cyanide hydratase was constructed to remove cyanogenic glycosides in edible plants. The β-glucosidase could hydrolyze cyanogenic glycosides into cyanide and its gene (Glu) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). A cyanide hydratase could catalyze cyanide to formamide and its...
March 2012
In silico identification of BIM-1 (2-methyl-1H-indol-3-yl) as a potential therapeutic agent against elevated protein kinase C beta associated diseases
Protein kinase C beta is involved in apoptosis, transcription regulation, B-cell activation, regulation of the B-cell receptor signalosome and B-cell survival by regulating BCR-induced NF-kappa-B activation. Increased activation of PKC-βI along with overproduction of diacylglycerol (DAG) and vascular endothelial growth factor (VEGF) participate in pathogenesis of various aliments like asthma, cancer...
March 2012
Hematological and enzymatic responses of Nile tilapia Oreochromis niloticus during short and long term sublethal exposure to zinc
Freshwater fish Oreochromis niloticus were exposed to sublethal concentrations of zinc for short and long terms. Responses of hematological parameters which include red blood cells count (RBC) and hemoglobin content (Hb) were investigated. Serumaspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities werealso determined. Two hundred and...
March 2012
Toxicological effects of arsenic exposure in a freshwater teleost fish, Channa punctatus
High concentration of arsenic in groundwater in the north-eastern states of India has become a major cause of concern. Inorganic arsenic of geological origin is found in groundwater used as drinking-water in several parts of the world. Arsenic is used in various industries and agriculture and excessive arsenic finds its way into lakes and rivers. Since arsenic is a known human carcinogen,...
March 2012
The influence of biopsy method on the survival rates of sexed and cryopreserved bovine embryos
The purpose of this research was to find the most suitable embryo biopsy technique that would yield the highest pregnancy rates after the transfer of sexed frozen-thawed bovine embryos. Three methods of embryo biopsy (needle, blade or aspiration) were applied on 120 bovine embryos divided into 3 batches (n = 40). Subsequently, the embryos were frozen/thawed and transferred to synchronized recipients....
March 2012
Three novel antioxidants from Cinnamomum plants
In this study, we identified three novel antioxidants, subamolide C (1), subamolide E (2) and isokotomolide A (3) from the constitutes of Cinnamomum subavenium andCinnamomum kotoense. The radical scavenging properties and two more antioxidant activities of the three compounds were examined. All compounds presented effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging...
March 2012
Antimicrobial susceptibilities of avian Escherichia coli isolates in Tabriz, Iran
Colibacillosis is a poultry disease of economic importance in Iran and all around the world. The aim of this study is to test the antibiotic sensitivity of Escherichia colistrains which were isolated in Tabriz. A total of 100 E. coli strains isolated from aviancolibacillosis of 50 farms from 2008 to 2009 in Tabriz, were investigated for antimicrobial susceptibility. Antimicrobial resistance of...
March 2012
The influence of cross-breeding Zlotnicka Spotted native breed sows with boars of Duroc (D) and Polish Large White (PLW) breeds on meat quality
The aim of the study was to investigate the influence of cross-breeding of Zlotnicka Spotted sows with Duroc and Polish Large White boars on the quality of meat of crossbred fatteners. The investigation was done on 50 carcasses of fatteners from four genetic groups: Zlotnicka Spotted x Zlotnicka Spotted (n = 20), Zlotnicka Spotted x Polish Large White (n = 10), Zlotnicka Spotted x Duroc (n = 10) and Zlotnicka...
March 2012
Variation of the platelet indices of dromedary camel (Camelus dromedarius) with age, sex and breed
The objective of this study was to determine reference intervals for platelet indices and hematological parameters in Saudi Arabian dromedary camels. Two hundred and twenty one apparently healthy dromedaries of Majahim (51 males and 31 females) and Maghatir (99 males and 40 males) breeds aged 1 - >10 yrs were investigated. Whole blood samples from the camels were analyzed for platelet...
March 2012
Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing
The paper aims to present a bovine model for human embryo sexing. Cows were super-ovulated, artificially inseminated and embryos were recovered 7 days later. Embryo biopsy was performed; DNA was extracted from blastomeres and amplified using bovine-specific and bovine-Y-chromosomespecific primers, followed by agarose gel electrophoresis. Embryos were then transferred to synchronized recipients and PCR...
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